Human Pro- Collagen II Antibody

Catalog #: MAB3615 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB3615-100
MAB3615-SP

Human Pro-Collagen II ELISA Standard Curve.

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All Collagen II Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Pro- Collagen II Antibody Summary

Species Reactivity

Human

Specificity

Detects human Pro-Collagen II in direct ELISAs.

Source

Recombinant Monoclonal Mouse IgG_2A Clone # 819427R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Pro-Collagen II
Gln26-Arg266 and Gly957-Leu1487
Accession # P02458

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Pro-Collagen II Monoclonal Antibody (Catalog # MAB36151).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Pro-Collagen II DuoSet ELISA Kit (Catalog # DY7589-05) for convenient development of a sandwich ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human Pro-Collagen II ELISA Standard Curve. Recombinant Human Pro-Collagen II protein was serially diluted 2-fold and captured by Mouse Anti-Human Pro-Collagen II Monoclonal Antibody (Catalog # MAB36151) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Pro-Collagen II Monoclonal Antibody (Catalog # MAB3615) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Collagen II

Type II collagen is an essential component of cartilage. It is essential for skeletal development and for the ability of cartilage to resist compressive forces. Type II collagen is a homotrimer of a1(II) chains. Defects in Type II collagen are the cause of numerous diseases, including chondrodysplasias and arthritis (1). This recombinant mini pro-a1(II) collagen consists of the N‑terminal propeptide, 108 Gly-X-Y repeats, and the C‑terminal propeptide. Proteolytic processing of the C‑terminal propeptide by the procollagen C-proteinase (BMP-1) releases chondrocalcin, a calcium binding protein.

References

Kuivaniemi H. et al. (1997) Hum. Mutat. 9:300.

Entrez Gene IDs

1280 (Human); 12824 (Mouse); 25412 (Rat)

Alternate Names

Alpha-1 type II collagen; ANFH; AOM; cartilage collagen; Chondrocalcin; COL11A3; COL2A1; Collagen 2; collagen alpha-1(II) chain; Collagen II; collagen II, alpha-1 polypeptide; collagen type 2; collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphysealdysplasia, congenital); collagen, type II, alpha 1; Collagen-2; MGC131516; SEDC