Human Flt-3/Flk-2 Antibody

Catalog #: MAB8121 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8121-SP
MAB8121-100
MAB8121-500

1 Image

All Flt-3/Flk-2 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Flt-3/Flk-2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Flt-3/Flk-2 in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant mouse (rm) Flt‑3, rmPDGF R alpha, rhPDGF R beta, or rhSCF R.

Source

Monoclonal Mouse IgG₁ Clone # 66907

Purification

Protein A or G purified from ascites

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Flt-3/Flk-2
Asn27-Asn541
Accession # P36888

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Flt‑3/Flk‑2 Fc Chimera (Catalog # 368-ST)

ELISA

This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human Flt‑3/Flk‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF812).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Flt-3/Flk-2 DuoSet ELISA Kit (Catalog # DY812-05) for convenient development of a sandwich ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

View Larger

Human Flt‑3/Flk‑2 ELISA Standard Curve. Recombinant Human Flt‑3/Flk‑2 protein was serially diluted 2-fold and captured by Mouse Anti-Human Flt‑3/Flk‑2 Monoclonal Antibody (Catalog # MAB8121) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human Flt‑3/Flk‑2 Antigen Affinity-purified Polyclonal Antibody (AF812) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Flt-3/Flk-2

The Flt-3 (fms-like tyrosine kinase) receptor, also named Flk-2 (fetal liver kinase) and Stk-1(stem cell tyrosine kinase) is a member of the class III subfamily of receptor tyrosine kinases that also includes KIT, the receptor for SCF and FMS, the receptor for M-CSF. The extracellular region of these receptors contains five immunoglobulin-like domains and the intracellular region contains a split kinase domain. Human Flt-3 cDNA encodes a 993 amino acid (aa) residue type I membrane protein with a 26 aa residue signal peptide, a 515 aa extracellular domain with 10 potential N-linked glycosylation sites, a 21 aa residue transmembrane domain and a 431 aa residue cytoplasmic domain. Mouse Flt-3 has also been cloned and shown to share 85% amino acid sequence identity with human Flt-3. Flt-3 expression has been detected in various tissues, including placenta, gonads, and tissues of nervous and hematopoietic origin. Among hematopoietic cells, the expression of Flt-3 was found to be restricted to the highly enriched stem/progenitor cell populations. The ligand for Flt-3 (FL) has been identified to be a transmembrane protein with structural homology to M-CSF and SCF. Recombinant soluble Flt-3/Fc chimeric protein has been shown to bind FL with high affinity and is a potent FL antagonist.

References

Rosnet, O. et al. (1996) Acta. Haemato. 95:218.
Drexler, H.G. (1996) Leukemia 10:588.

Long Name

fms-like Tyrosine Kinase 3

Entrez Gene IDs

2322 (Human); 14255 (Mouse)

Alternate Names

CD135 antigen; CD135; EC 2.7.10; fetal liver kinase 2; FL cytokine receptor; FLK2; Flk-2; FLT3 receptor tyrosine kinase; Flt3; Flt-3; Fms-like tyrosine kinase 3; fms-related tyrosine kinase 3; growth factor receptor tyrosine kinase type III; Stem cell tyrosine kinase 1; STK-1; STK1EC 2.7.10.1; Tyrosine-protein kinase receptor FLT3