Human APRIL/TNFSF13 Antibody

Catalog #: MAB8844 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8844-100
MAB8844-SP

Human APRIL/TNFSF13 ELISA Standard Curve.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human APRIL/TNFSF13 Antibody Summary

Species Reactivity

Human

Specificity

Detects human APRIL/TNFSF13 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 540218

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human APRIL/TNFSF13
Lys110-Leu250
Accession # Q8NFH7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human APRIL/TNFSF13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF884).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human APRIL/TNFSF13 DuoSet ELISA Kit (Catalog # DY884B) for convenient development of a sandwich ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human APRIL/TNFSF13 ELISA Standard Curve. Recombinant Human APRIL/TNFSF13 protein was serially diluted 2-fold and captured by Mouse Anti-Human APRIL/TNFSF13 Monoclonal Antibody (Catalog # MAB8844) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human APRIL/TNFSF13 Antigen Affinity-purified Polyclonal Antibody (AF884 ) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: APRIL/TNFSF13

APRIL (a proliferation inducing ligand), also known as TNFSF13, TALL2, and TRDL1, is a member of the TNF ligand superfamily. It is synthesized as a 32 kDa type II transmembrane protein which is cleaved by furin in the Golgi to release a 17 kDa soluble molecule. Secreted APRIL consists almost entirely of a single TNF homology domain. Little or no transmembrane APRIL is expressed on the cell surface. Alternate splicing generates isoforms with short deletions at the N- or C‑terminus. Both APRIL and the closely related protein BAFF signal through the TNF superfamily receptors TACI and BCMA to promote cellular proliferation and protect from apoptosis. APRIL can form bioactive heterotrimers with BAFF. A bioactive cell surface-expressed protein known as TWEPRIL consists of the intracellular domain, transmembrane segment, and stalk region of TWEAK fused to the TNF homology domain of APRIL. Human APRIL shares 85% amino acid sequence identity with mouse and rat APRIL.

Long Name

A Proliferation-inducing Ligand

Entrez Gene IDs

8741 (Human); 69583 (Mouse); 287437 (Rat)

Alternate Names

A proliferation-inducing ligand; APRIL; APRILFLJ57090; CD256 antigen; CD256; TALL2; TALL-2; TNF- and APOL-related leukocyte expressed ligand 2; TNF-related death ligand 1; TNFSF13; TRDL1; TRDL-1; tumor necrosis factor (ligand) superfamily, member 13; tumor necrosis factor ligand superfamily member 13; tumor necrosis factor-like protein ZTNF2; tumor necrosis factor-related death ligand-1; ZTNF2