Viral Dengue Virus 1 NS1 Antibody

Catalog #: MAB94381 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB94381-100
MAB94381-SP

Viral Dengue Virus 1 NS1 ELISA Standard Curve.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Viral Dengue Virus 1 NS1 Antibody Summary

Species Reactivity

Viral

Specificity

Detects viral Dengue Virus 1 NS1 protein in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 973604

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human embryonic kidney cell line HEK293-derived viral Dengue Virus 1 NS1 protein
Asp776-Ala1127
Accession # P17763

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Viral Dengue Virus 1 NS1 Monoclonal Antibody (Catalog # MAB9438).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Viral Dengue Virus 1 NS1 ELISA Standard Curve. Recombinant Viral Dengue Virus 1 NS1 protein was serially diluted 2-fold and captured by Mouse Anti-Viral Dengue Virus 1 NS1 Monoclonal Antibody (Catalog # MAB9438) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Viral Dengue Virus 1 NS1 Monoclonal Antibody (Catalog # MAB94381) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dengue Virus 1 NS1

Dengue virus type 1 non-structural protein 1 (DENV1 NS1) is an approximately 50 kDa viral glycoprotein (1). Dengue viruses are mosquito-borne flaviviruses that exist in nature as four distinct serotypes (DENV1-4) (2). Dengue virus NS1 can be found associated with cell membranes, within cells and at the cell surface, or as secreted extracellular species. The secreted form of NS1 is known to be hexameric (1). Within cells, NS1 plays a significant role in virus replication and has been shown to co-localize with dsRNA and other components of viral replication complexes in viral-induced vesicle packets (1,3). Cell surface-associated and secreted NS1 are profoundly immunogenic, and both the protein itself and the antibodies it elicits have been shown to contribute to either protection or pathogenesis and innate immune evasion (1, 3). Mature DENV1 NS1 contains 352 amino acids (aa) and consists of 2 conserved N-glycosylation sites and 12 invariant cysteine residues that form six intramolecular disulfide bonds (3).Mature DENV1 NS1 shares 73%, 80%, and 69% aa sequence identity with DENV2, DENV3, and DENV4 NS1, respectively.

References