Human Peptide YY Antibody

Catalog #: MAB94561 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB94561-100
MAB94561-SP

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Human Peptide YY Antibody Summary

Species Reactivity

Human

Specificity

Detects human YY synthetic peptide in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 1011012

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human Peptide YY synthetic peptide

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human Peptide YY Monoclonal Antibody (Catalog # MAB94562).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human Peptide YY ELISA Standard Curve. Recombinant Human Peptide YY protein was serially diluted 2-fold and captured by Mouse Anti-Human Peptide YY Monoclonal Antibody (Catalog # MAB94561) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Peptide YY Monoclonal Antibody (Catalog # MAB94562) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Peptide YY

Peptide YY (PYY), a secretory peptide, is expressed by L cells (enteroendocrine cells) in the small and large intestine, Islet of Langerhans cells, testis, and in some central and peripheral neurons. Because PYY is released from L cells after feeding, it is thought to produce a satiety signal. While the regulation of appetite by PYY and GLP-1 have been studied extensively, recent evidence supports a role for PYY in Islet of Langerhans beta cell survival. PYY secretion is also modulated by gut microbiota, and levels of colonic PYY have reduced in rats with DSS-induced colitis, and in humans with inflammatory bowel disease (IBD), while circulating PYY increased in IBD.

Entrez Gene IDs

5697 (Human); 217212 (Mouse); 287730 (E. faecalis)

Alternate Names

Peptide tyrosine tyrosine; Peptide YY; Peptide-YY; PYY; PYY1; PYY-I

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

FAQs

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