Mouse Fibroblast Activation Protein alpha /FAP Antibody
Mouse Fibroblast Activation Protein alpha /FAP Antibody Summary
Leu26-Asp761
Accession # P97321
Applications
This antibody functions as an ELISA capture antibody when paired with Rat Anti-Mouse Fibroblast Activation Protein alpha /FAP Monoclonal Antibody (Catalog # MAB97271).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Fibroblast Activation Protein alpha/FAP in C2C12 Mouse Cell Line by Flow Cytometry. C2C12 mouse myoblast cell line was stained with Rat Anti-Mouse Fibroblast Activation Protein alpha/FAP Monoclonal Antibody (Catalog # MAB9727, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.
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Fibroblast Activation Protein alpha /FAP in C2C12 Mouse Cell Line. Fibroblast Activation Protein a/FAP was detected in immersion fixed C2C12 mouse myoblast cell line using Rat Anti-Mouse Fibroblast Activation Protein a/FAP Monoclonal Antibody (Catalog # MAB9727) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Fibroblast Activation Protein alpha/FAP
FAP (also known as seprase) is a 95 kDa Type II transmembrane serine protease that is structurally related to dipeptidyl peptidase IV (DPPIV/CD26) (1, 2). Within the extracellular domain, mouse FAP shares 90% and 97% amino acid (aa) sequence identity with human and rat FAP, respectively (3, 4). Alternative splicing of mouse FAP generates isoforms with a 33 aa or 5 aa deletion in the extracellular juxtamembrane region (3). FAP is expressed on reactive stromal fibroblasts in tumor tissue and wound healing and on synoviocytes in rheumatoid arthritis (1, 5-7). It exhibits dipeptidyl peptidase activity with substrate specificity similar to DPPIV, which is specific for N-terminal Xaa-Pro sequences (5, 8). FAP is also an endopeptidase that can degrade Gelatin, Collagens I and IV, Fibronectin, and Laminin (1, 5, 8) as well as several peptide hormones (e.g. Neuropeptide Y, Brain Natriuretic Peptide, Substance P, Peptide YY, and Incretins) (9). The enzymatic activity is dependent on FAP association with DPPIV on the cell surface (5, 8, 10, 11). The matrix-dedgrading activity of FAP contributes to tumor cell migration and invasion (10-13). In addition, FAP can enhance tumor cell growth by limiting the development of anti-tumor immunity (14).
- Zi, F. et al. (2015) Mol. Med. Rep. 11:3203.
- Pineiro-Sanchez, M.L. et al. (1997) J. Biol. Chem. 272:7595.
- Niedermeyer, J. et al. (1997) Int. J. Cancer 71:383.
- Scanlan, M.J. et al. (1994) Proc. Natl. Acad. Sci. USA 91:5657.
- Park, J.E. et al. (1999) J. Biol. Chem. 274:36505.
- Rettig, W.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3110.
- Bauer, S. et al. (2006) Arthritis Res. 8:R171.
- Aertgeerts, K. et al. (2005) J. Biol. Chem. 280:19441.
- Keane, F.M. et al. (2011) FEBS J. 278:1316.
- Ghersi, G. et al. (2006) Cancer Res. 66:4652.
- Ghersi, G. et al. (2002) J. Biol. Chem. 277:29231.
- Cheng, J.D. et al. (2005) Mol. Cancer Ther. 4:351.
- Cheng, J.D. et al. (2002) Cancer Res. 62:4767.
- Kraman, M. et al. (2010) Science 330:827.
Product Datasheets
Citations for Mouse Fibroblast Activation Protein alpha /FAP Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Stromal depletion by TALEN-edited universal hypoimmunogenic FAP-CAR T cells enables infiltration and anti-tumor cytotoxicity of tumor antigen-targeted CAR-T immunotherapy
Authors: Shipra Das, Julien Valton, Philippe Duchateau, Laurent Poirot
Frontiers in Immunology
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Fibroblast-like synoviocytes orchestrate daily rhythmic inflammation in arthritis
Authors: Downton, P;Dickson, SH;Ray, DW;Bechtold, DA;Gibbs, JE;
Open biology
Species: Transgenic Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Tumor Suppression by Anti-Fibroblast Activation Protein Near-Infrared Photoimmunotherapy Targeting Cancer-Associated Fibroblasts
Authors: Glabman, RA;Olkowski, CP;Minor, HA;Bassel, LL;Kedei, N;Choyke, PL;Sato, N;
Cancers
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Pancreatic tumor eradication via selective Pin1 inhibition in cancer-associated fibroblasts and T lymphocytes engagement
Authors: J Liu, Y Wang, C Mu, M Li, K Li, S Li, W Wu, L Du, X Zhang, C Li, W Peng, J Shen, Y Liu, D Yang, K Zhang, Q Ning, X Fu, Y Zeng, Y Ni, Z Zhou, Y Liu, Y Hu, X Zheng, T Wen, Z Li, Y Liu
Nature Communications, 2022-07-25;13(1):4308.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
In utero estrogenic endocrine disruption alters the stroma to increase extracellular matrix density and mammary gland stiffness
Authors: C Wormsbaech, AR Hindman, A Avendano, M Cortes-Med, CE Jones, A Bushman, L Onua, CE Kovalchin, AR Murphy, HL Helber, A Shapiro, K Voytovitch, X Kuang, R Aguilar-Va, JL Leight, JW Song, CJ Burd
Breast Cancer Res., 2020-05-05;22(1):41.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
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