Human PEG10 Antibody

Catalog #: MAB99751 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB99751-100
MAB99751-SP

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Detection of PEG-10 in Human HepG2 cell line by Flow Cytometry.

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All PEG10 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PEG10 Antibody Summary

Species Reactivity

Human

Specificity

Detects human PEG10 in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 981902

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Synthetic peptide containing human PEG10
Accession # Q86TG7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

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Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human PEG10 Monoclonal Antibody (Catalog # MAB9975).

This product is intended for assay development on various assay platforms requiring antibody pairs.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of PEG-10 antibody in Human HepG2 cell line antibody by Flow Cytometry.

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Detection of PEG-10 in Human HepG2 cell line by Flow Cytometry. HepG2 human hepatoma cell line was stained with Mouse Anti-Human PEG-10 Monoclonal Antibody (Catalog # MAB99751, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PEG10

Paternally Expressed Gene-10, or PEG10 (a.k.a Retrotransposon-derived protein PEG10, EDR, MART2, MEF3L1 and RGAG3) is a 708 amino acids (aa) protein encoded by the PEG10 gene. PEG-10 Inhibits the TGF-beta signaling by interacting with the TGF-beta receptor ALK1, possibly playing a key role in epithelial-mesenchymal transition (EMT). PEG-10 has been suggested to participate in cell proliferation, differentiation and cell apoptosis processes and identified as a prognostic biomarker for progression of several carcinomas, like breast, lung, liver and pancreatic carcinomas.

Long Name

Paternally Expressed 10

Entrez Gene IDs

23089 (Human); 170676 (Mouse); 678843 (Rat)

Alternate Names

EDR; embryonal carcinoma differentiation regulated; Embryonal carcinoma differentiation-regulated protein; HB-1; KIAA1051EDR; Mammalian retrotransposon-derived protein 2; Mar2; Mart2; MEF3 Like 1; MEF3L; MEF3L1; Myelin expression factor 3-like protein 1; paternally expressed 10; Paternally expressed gene 10 protein; PEG10; retrotransposon gag domain containing 3; Retrotransposon gag domain-containing protein 3; Retrotransposon-derived gag-like polyprotein; retrotransposon-derived protein PEG10; RGAG3; RGAG3MEF3-like protein 1; RTL2; SIRH1; Ty3/Gypsy-like protein