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Rat IgG VisUCyte HRP Polymer Antibody

Catalog #: VC005
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
VC005-025
VC005-050
VC005-125
VC005-250
Detection of HSP27 in Human Ovary.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat IgG VisUCyte HRP Polymer Antibody Summary

Species Reactivity
Rat
Specificity
Detects heavy ( gamma ) chains on rat IgG and light chains on all rat immunoglobulins, based on IEP. No cross-reactivity is observed with non-immunoglobulin rat serum proteins or IgG from human or mouse, based on IEP. VisUCyte™ HRP Polymer provides high sensitivity with low background in IHC.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Rat IgG
Formulation
Supplied as a solution in PBS with preservative.
Label
HRP Polymer(Excitation= nm, Emission= nm)

Applications

Recommended Concentration
Sample
Immunohistochemistry
Prediluted, ready to use with formalin fixed paraffin-embedded and frozen sections. Compatible with most commonly used fixatives.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Detection of HSP27 in Human Ovary. View Larger

Detection of HSP27 in Human Ovary. HSP27 was detected in paraffin-embedded sections human ovary using Rat Anti-Human HSP27 Monoclonal Antibody (Catalog # MAB15801) with Anti-Rat IgG VisUCyte HRP Polymer Antibody (Catalog # VC005). Tissue was stained with DAB (brown color) and counterstained with hematoxylin (blue color). View our protocol for theVisUCyte HRP Polymer Detection Reagent.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

VisUCyte™ HRP Polymer is a biotin-free detection reagent which overcomes problems of avidin-biotin detection chemistry, such as endogenous biotin staining and the need to do additional quenching of endogenous biotin and avidin present in some tissues. With VisUCyte HRP Polymer it is possible to achieve specific staining in cells and tissue much faster than the conventional avidin-biotin-HRP procedure. Due the high sensitivity of VisUCyte™ HRP Polymer, the working concentration of primary antibodies may be 2-3 times lower compared to the conventional avidin-biotin-HRP protocol.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Citations for Rat IgG VisUCyte HRP Polymer Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. The Role of CCL24 in Primary Sclerosing Cholangitis: Bridging Patient Serum Proteomics to Preclinical Data
    Authors: Greenman, R;Snir, T;Katav, A;Aricha, R;Mishalian, I;Hay, O;Frankel, M;Lawler, J;Saffioti, F;Pinzani, M;Thorburn, D;Peled, A;Mor, A;Vaknin, I;
    Cells
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  2. Depleting Ly6G Positive Myeloid Cells Reduces Pancreatic Cancer-Induced Skeletal Muscle Atrophy
    Authors: MR Deyhle, CS Callaway, D Neyroud, AC D'Lugos, SM Judge, AR Judge
    Cells, 2022-06-10;11(12):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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