Mouse BAFF/BLyS/TNFSF13B DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
Customers also Viewed
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Scientific Data
Product Datasheets
Preparation and Storage
Background: BAFF/BLyS/TNFSF13B
BAFF (B-cell activating factor), also known as BLyS, TALL-1, THANK, and TNFSF13B, is a TNF superfamily ligand that plays a critical role in the development and survival of B lineage cells. It can be expressed as a homotrimer or as a heteromer in association with the related protein APRIL. It is produced by many hematopoietic cell types, and a soluble form can be released by activated neutrophils. Both BAFF and APRIL signal through the receptors BCMA and TACI, and BAFF additionally signals through BAFF R. Mice that overexpress BAFF exhibit elevated B cell numbers, increased formation and size of germinal centers, and symptoms of autoimmunity. In addition, BAFF costimulates T cell activation, promotes a Th1 biased immune response, and promotes the expansion of Treg cells.
Citations for Mouse BAFF/BLyS/TNFSF13B DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
9
Citations: Showing 1 - 9
Filter your results:
Filter by:
-
BAFF and APRIL counterregulate susceptibility to inflammation-induced preterm birth
Authors: JR Doll, ME Moreno-Fer, TE Stankiewic, JL Wayland, A Wilburn, B Weinhaus, CA Chougnet, D Giordano, M Cappellett, P Presicce, SG Kallapur, N Salomonis, T Tilburgs, S Divanovic
Cell Reports, 2023-04-05;42(4):112352.
Species: Mouse
Sample Types:
-
B cell activating factor (BAFF) from neutrophils and dendritic cells is required for protective B cell responses against Salmonella typhimurium infection
Authors: R Kuley, KE Draves, DH Fuller, NV Giltiay, EA Clark, D Giordano
PLoS ONE, 2021-10-27;16(10):e0259158.
Species: Mouse
Sample Types: Serum
-
Circulating CD138 enhances disease progression by augmenting autoreactive antibody production in a mouse model of systemic lupus erythematosus
Authors: L Liu, M Akkoyunlu
The Journal of Biological Chemistry, 2021-08-06;0(0):101053.
Species: Mouse
Sample Types: Serum
-
Variable dependency on BAFF in IgG antibody production during Leishmania infection
Authors: N Azuma, S Omachi, W Hanazawa, A Morimoto, C Sanjoba, Y Matsumoto, W Fujii, Y Goto
Parasitol. Int., 2019-10-15;74(0):101997.
Species: Mouse
Sample Types: Serum
-
ORMDL3 Facilitates the Survival of Splenic B Cells via an ATF6?-Endoplasmic Reticulum Stress-Beclin1 Autophagy Regulatory Pathway
Authors: J Dang, X Bian, X Ma, J Li, F Long, S Shan, Q Yuan, Q Xin, Y Li, F Gao, Y Gong, Q Liu
J. Immunol., 2017-07-26;0(0):.
Species: Mouse
Sample Types: Serum
-
B-cell activating factor deficiency suppresses splenomegaly during Leishmania donovani infection
Authors: S Omachi, W Fujii, N Azuma, A Morimoto, C Sanjoba, Y Matsumoto, Y Goto
Biochem. Biophys. Res. Commun., 2017-06-03;0(0):.
Species: Mouse
Sample Types: Serum
-
Silica-Triggered Autoimmunity in Lupus-Prone Mice Blocked by Docosahexaenoic Acid Consumption
PLoS ONE, 2016-08-11;11(8):e0160622.
Species: Mouse
Sample Types: BALF
-
The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells.
Authors: Saulep-Easton D, Vincent F, Quah P, Wei A, Ting S, Croce C, Tam C, Mackay F
Leukemia, 2015-07-03;30(1):163-72.
Species: Mouse
Sample Types: Cell Culture Supernates
-
B cell homeostasis and follicle confines are governed by fibroblastic reticular cells.
Authors: Cremasco, Viviana, Woodruff, Matthew, Onder, Lucas, Cupovic, Jovana, Nieves-Bonilla, Janice M, Schildberg, Frank A, Chang, Jonathan, Cremasco, Floriana, Harvey, Christop, Wucherpfennig, Kai, Ludewig, Burkhard, Carroll, Michael, Turley, Shannon
Nat Immunol, 2014-08-24;15(10):973-81.
Species: Mouse
Sample Types: Serum
FAQs
No product specific FAQs exist for this product, however you may
View all ELISA FAQsDuoSet Ancillary Reagent Kits
Supplemental ELISA Products
Reviews for Mouse BAFF/BLyS/TNFSF13B DuoSet ELISA
Average Rating: 5 (Based on 2 Reviews)
Have you used Mouse BAFF/BLyS/TNFSF13B DuoSet ELISA?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
