Mouse Gas6 Quantikine ELISA Kit

Name: Mouse Gas6 Quantikine ELISA Kit
Brand: R&D Systems
SKU: MGAS60

Catalog #: MGAS60
1 Citation Datasheet / COA / SDS

Discontinued Product

MGAS60 has been discontinued.

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All Gas6 ELISAs

Product Details

Citations (1)

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Mouse Gas6 Quantikine ELISA Kit Summary

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)

Sensitivity

0.027 ng/mL

Assay Range

0.2 - 10 ng/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant mouse Gas6

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse Gas6 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse Gas6 in cell culture supernates, cell lysates, serum, and plasma. It contains NS0-expressed recombinant mouse Gas6 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse Gas6 showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Gas6.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	0.451	1.28	3.93	0.465	1.3	3.98
Standard Deviation	0.023	0.052	0.129	0.024	0.054	0.285
CV%	5.1	4.1	3.3	5.2	4.2	7.2

Recovery

The recovery of Gas6 spiked into cell culture samples were evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Samples (n=4)	111	103-118

Linearity

To assess the linearity of the assay, samples containing high concentrations of mouse Gas6 were serially diluted with Calibrator Diluent RD5-26 (1X) to produce samples with values within the dynamic range of the assay.

Scientific Data

N/A

View Larger

Mouse Gas6 Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Gas6

Gas6 (Growth Arrest Specific 6) is a secreted protein that contains an extensively gamma-carboxylated N-terminal Gla domain and is dependent upon vitamin K for activity. Gas6 signals through the receptors Axl, Dtk/Tyro3, and Mer and is inhibited by shed soluble forms of Axl and Mer. Gas6 protects cells from stress-induced apoptosis and promotes phagocytosis of apoptotic cell debris, promotes platelet activation but inhibits inflammatory cytokine production, and inhibits VEGF-induced angiogenesis, and regulates tumor cell invasiveness.

Long Name:

Growth-arrest-specific Protein 6

Entrez Gene IDs:

2621 (Human); 14456 (Mouse)

Alternate Names:

AXLLG; AXLLGAXL stimulatory factor; AXSFAXL receptor tyrosine kinase ligand; DKFZp666G247; FLJ34709; Gas6; GAS-6; growth arrest-specific 6; growth arrest-specific protein 6

Related Research Areas

Citation for Mouse Gas6 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Exogenous testosterone alleviates cardiac fibrosis and apoptosis via Gas6/Axl pathway in the senescent mice
Authors: FF Chen, FQ Song, YQ Chen, ZH Wang, YH Li, MH Liu, Y Li, M Song, W Zhang, J Zhao, M Zhong
Exp. Gerontol., 2019-01-30;0(0):.
Species: Mouse
Sample Types: Serum