Mouse Total MMP-9 DuoSet ELISA

Catalog #: DY6718
12 Citations Datasheet / COA / SDS

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DY6718

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Citations (12)

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Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Mouse Total MMP-9 DuoSet ELISA Summary

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Sample Volume Required

100 µL

Assay Range

78.1 - 5,000 pg/mL

Sufficient Materials

For fifteen 96-well plates*

Specificity

Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse Total MMP-9. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits

Kit Content

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H₂O₂) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H₂SO₄ (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

N/A

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Mouse Total MMP-9 ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MMP-9

The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).

Long Name:

Matrix Metalloproteinase 9

Entrez Gene IDs:

4318 (Human); 17395 (Mouse); 81687 (Rat); 102117693 (Cynomolgus Monkey)

Alternate Names:

92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; EC 3.4.24; EC 3.4.24.35; Gelatinase B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9; matrix metalloproteinase 9; matrix metalloproteinase-9; MMP9; MMP-9; type V collagenase

Citations for Mouse Total MMP-9 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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Keratin 15 protects against cigarette smoke-induced epithelial mesenchymal transformation by MMP-9
Authors: Zhu, W;Han, L;Wu, Y;Tong, L;He, L;Wang, Q;Yan, Y;Pan, T;Shen, J;Song, Y;Shen, Y;Zhu, Q;Zhou, J;
Respiratory research
Species: Transgenic Mouse, Mouse
Sample Types: BALF
Knocking-Down CD147/EMMPRIN Expression in CT26 Colon Carcinoma Forces the Cells into Cellular and Angiogenic Dormancy That Can Be Reversed by Interactions with Macrophages
Authors: G Feigelman, E Simanovich, P Brockmeyer, MA Rahat
Biomedicines, 2023-03-02;11(3):.
Species: Mouse
Sample Types: Cell Culture Supernates
Efficacy of a Probiotic and Herbal Supplement in Models of Lung Inflammation
Authors: NM Wenger, L Qiao, T Nicola, Z Nizami, X Xu, KA Willis, N Ambalavana, A Gaggar, CV Lal
Microorganisms, 2022-10-28;10(11):.
Species: Mouse
Sample Types: BALF
Ocular microbiota promotes pathological angiogenesis and inflammation in sterile injury-driven corneal neovascularization
Authors: HJ Lee, CH Yoon, HJ Kim, JH Ko, JS Ryu, DH Jo, JH Kim, D Kim, JY Oh
Mucosal Immunology, 2022-08-19;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Neutrophil extracellular traps modulate inflammatory markers and uptake of oxidized LDL by human and murine macrophages
Authors: A Conforti, T Wahlers, A Paunel-Gör
PLoS ONE, 2021-11-19;16(11):e0259894.
Species: Mouse
Sample Types: Cell Culture Supernates
Anti-inflammatory activity of lefamulin versus azithromycin and dexamethasone in vivo and in vitro in a lipopolysaccharide-induced lung neutrophilia mouse model
Authors: M Hafner, S Paukner, WW Wicha, B Hrva?i?, M Cedilak, I Faraho, SP Gelone
PLoS ONE, 2021-09-29;16(9):e0237659.
Species: Mouse
Sample Types: Tissue Homogenates
In Vitro Evaluation of the Anti-Inflammatory Effect of KMUP-1 and In Vivo Analysis of Its Therapeutic Potential in Osteoarthritis
Authors: SE Huang, E Sulistyowa, YY Chao, BN Wu, ZK Dai, JH Hsu, JL Yeh
Biomedicines, 2021-05-28;9(6):.
Species: Mouse
Sample Types: Cell Culture Supernates
Yersinia pseudotuberculosisï¿½YopH targets SKAP2-dependent and independent signaling pathways to block neutrophil antimicrobial mechanisms during infection
Authors: L Shaban, GT Nguyen, BD Mecsas-Fax, KD Swanson, S Tan, J Mecsas
PLoS Pathog., 2020-05-11;16(5):e1008576.
Species: Mouse
Sample Types: Cell Culture Supernates
Oral administration of EPA-rich oil impairs collagen reorganization due to elevated production of IL-10 during skin wound healing in mice
Authors: B Burger, CMC Kühl, T Candreva, RDS Cardoso, JR Silva, BG Castelucci, SR Consonni, HL Fisk, PC Calder, MAR Vinolo, HG Rodrigues
Sci Rep, 2019-06-24;9(1):9119.
Species: Mouse
Sample Types: Tissue Homogenates
Experimental atopic dermatitis depends on IL-33R signaling via MyD88 in dendritic cells
Authors: C Li, I Maillet, C Mackowiak, C Viala, F Di Padova, M Li, D Togbe, V Quesniaux, Y Lai, B Ryffel
Cell Death Dis, 2017-04-06;8(4):e2735.
Species: Mouse
Sample Types: Tissue Homogenates

Carcinoembryonic antigen-related cell adhesion molecule 1 inhibits MMP-9-mediated blood-brain-barrier breakdown in a mouse model for ischemic stroke.
Authors: Ludewig P, Sedlacik J, Gelderblom M, Bernreuther C, Korkusuz Y, Wagener C, Gerloff C, Fiehler J, Magnus T, Horst A
Circ Res, 2013-06-18;113(8):1013-22.
Species: Mouse
Sample Types: Cell Culture Supernates

Altered regulation of Toll-like receptor responses impairs antibacterial immunity in the allergic lung.
Authors: Habibzay, M, Saldana, J I, Goulding, J, Lloyd, C M, Hussell, T
Mucosal Immunol, 2012-05-02;5(5):524-34.
Species: Mouse
Sample Types: BALF