Recombinant Mouse Lumican Protein, CF Summary
Product Specifications
Optimal dilutions should be determined by each laboratory for each application. Also measured by its ability to modulate collagen fibrillogenesis.
Gln19-Asn338, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2745-LU
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 200 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Lumican
Lumican is a 40 kDa member of the family of small leucine-rich repeat proteoglycans (SLRPs) and the class II subfamily (1). Mouse Lumican is synthesized as a 338 amino acid (aa) precursor that contains an 18 aa signal sequence and a 320 aa mature chain (SwissProt #: 51885). The mature chain contains a negatively charged N-terminal domain containing sulfated tyrosine and disulfide bonds, 12 leucine-rich repeats (LRRs), four potential sites of N-linked glycosylation, and a carboxyl terminal domain containing two conserved cysteines (1). Mature mouse Lumican is 98%, 88%, and 70% aa identical to mature rat, human, and chick Lumican, respectively. SLRPs constitute an important fraction of noncollagenous extracellular matrix proteins (ECM) proteins (1, 2). Lumican is expressed in a variety of tissues, including skin, artery, lung, cornea, kidney, bone, aorta, and articular cartilage (1). Lumican’s role in vivo has been found using Lumican null mice. These mice have functional deficits including corneal opacity as well as skin and tendon fragility associated with disorganized and loosely packed collagen fibers (1, 3-6). The abnormal connective tissue phenotype seen in the Lumican null mice shows the importance of the role of Lumican in collagen fibrillogenesis (1). In addition to the control of collagen fibril assembly, Lumican has been shown to play a role in the regulation of cell proliferation (7, 8), migration (8 - 9), and adhesion (9). Lumican’s overexpression has been reported in carcinoid tumor, breast, colorectal, neuroendocrine, uterine cervical and pancreatic cancers (10).
- Nikitovic, D. et al. (2008) IUBMB Life 60:818.
- Blochberger, T.C. et al. (1992) J. Biol. Chem. 267:347.
- Chakravarti, S. et al. (1998) J. Cell Biol. 141:1277.
- Chakravarti, S. et al. (2000) Invest. Ophthalmol. Vis. Sci. 41:3365.
- Jepsen, K.J. et al. (2002) J. Biol. Chem. 277:35532.
- Chakravarti, S. et al. (2003) Invest. Ophthalmol. Vis. Sci. 44:2422.
- Vuillermoz, B. et al. (2004) Exp. Cell Res. 296:294
- Nikitovic, D. et al. (2008) FEBS J. 275:350.
- D’Onofrio, M.F. et al. (2008) Biochem. Biophys. Res. Commun. 365:266.
- Ishiwata, T. et al. (2007) Oncol. Rep. 18:537.
Citations for Recombinant Mouse Lumican Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Rab18 Drift in Lipid Droplet and Endoplasmic Reticulum Interactions of Adipocytes under Obesogenic Conditions
Authors: López-Alcalá, J;Soler-Vázquez, MC;Tercero-Alcázar, C;Sánchez-Ceinos, J;Guzmán-Ruiz, R;Malagón, MM;Gordon, A;
International journal of molecular sciences
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Lumican, an Exerkine, Protects against Skeletal Muscle Loss
Authors: HJ Cho, YS Lee, DA Kim, SA Moon, SE Lee, SH Lee, JM Koh
International Journal of Molecular Sciences, 2022-09-02;23(17):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Cleavage of proteoglycans, plasma proteins and the platelet-derived growth factor receptor in the hemorrhagic process induced by snake venom metalloproteinases
Authors: AF Asega, MC Menezes, D Trevisan-S, D Cajado-Car, L Bertholim, AK Oliveira, A Zelanis, SMT Serrano
Sci Rep, 2020-07-31;10(1):12912.
Species: Mouse
Sample Types: Recombinant Protein
Applications: Bioassay -
Prolonged exposure to extracellular lumican restrains pancreatic adenocarcinoma growth
Authors: X Li, Y Kang, D Roife, Y Lee, M Pratt, MR Perez, B Dai, EJ Koay, JB Fleming
Oncogene, 2017-05-22;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Determination of the sites of tyrosine O-sulfation in peptides and proteins.
Authors: Yu Y, Hoffhines AJ, Moore KL, Leary JA
Nat. Methods, 2007-06-10;4(7):583-8.
Species: Mouse
Sample Types:
Applications: Enzyme Assay Substrate
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