Recombinant Mouse Lumican Protein, CF

Catalog # Availability Size / Price Qty
2745-LU-050

Save on RUO reagents! Up to 30% off + FREE shipping. See Details.

R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
Citations (5)
FAQs
Supplemental Products
Reviews (1)

Recombinant Mouse Lumican Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of BCE C/D‑1b bovine corneal endothelial cells. When 5 x 104 cells per well are added to rmLumican coated plates (10 μg/mL, 100 μL/well) ≥ 50% will adhere after 50 minutes at 37 °C.
Optimal dilutions should be determined by each laboratory for each application. Also measured by its ability to modulate collagen fibrillogenesis.
Source
Mouse myeloma cell line, NS0-derived mouse Lumican protein
Gln19-Asn338, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
No results obtained: Gln19 predicted
Predicted Molecular Mass
37.3 kDa
SDS-PAGE
55-65 kDa, reducing conditions

Product Datasheets

You must select a language.

x

2745-LU

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2745-LU

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Background: Lumican

Lumican is a 40 kDa member of the family of small leucine-rich repeat proteoglycans (SLRPs) and the class II subfamily (1). Mouse Lumican is synthesized as a 338 amino acid (aa) precursor that contains an 18 aa signal sequence and a 320 aa mature chain (SwissProt #: 51885). The mature chain contains a negatively charged N-terminal domain containing sulfated tyrosine and disulfide bonds, 12 leucine-rich repeats (LRRs), four potential sites of N-linked glycosylation, and a carboxyl terminal domain containing two conserved cysteines (1). Mature mouse Lumican is 98%, 88%, and 70% aa identical to mature rat, human, and chick Lumican, respectively. SLRPs constitute an important fraction of noncollagenous extracellular matrix proteins (ECM) proteins (1, 2). Lumican is expressed in a variety of tissues, including skin, artery, lung, cornea, kidney, bone, aorta, and articular cartilage (1). Lumican’s role in vivo has been found using Lumican null mice. These mice have functional deficits including corneal opacity as well as skin and tendon fragility associated with disorganized and loosely packed collagen fibers (1, 3-6). The abnormal connective tissue phenotype seen in the Lumican null mice shows the importance of the role of Lumican in collagen fibrillogenesis (1). In addition to the control of collagen fibril assembly, Lumican has been shown to play a role in the regulation of cell proliferation (7, 8), migration (8 - 9), and adhesion (9). Lumican’s overexpression has been reported in carcinoid tumor, breast, colorectal, neuroendocrine, uterine cervical and pancreatic cancers (10).

References
  1. Nikitovic, D. et al. (2008) IUBMB Life 60:818.
  2. Blochberger, T.C. et al. (1992) J. Biol. Chem. 267:347.
  3. Chakravarti, S. et al. (1998) J. Cell Biol. 141:1277.
  4. Chakravarti, S. et al. (2000) Invest. Ophthalmol. Vis. Sci. 41:3365.
  5. Jepsen, K.J. et al. (2002) J. Biol. Chem. 277:35532.
  6. Chakravarti, S. et al. (2003) Invest. Ophthalmol. Vis. Sci. 44:2422.
  7. Vuillermoz, B. et al. (2004) Exp. Cell Res. 296:294
  8. Nikitovic, D. et al. (2008) FEBS J. 275:350.
  9. D’Onofrio, M.F. et al. (2008) Biochem. Biophys. Res. Commun. 365:266.
  10. Ishiwata, T. et al. (2007) Oncol. Rep. 18:537.
Entrez Gene IDs
4060 (Human); 17022 (Mouse)
Alternate Names
KSPG lumican; LDC; LUM; lumican proteoglycan; Lumican; SLRR2D; SLRR2DLDCKeratan sulfate proteoglycan lumican

Citations for Recombinant Mouse Lumican Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
Filter your results:

Filter by:

  1. Rab18 Drift in Lipid Droplet and Endoplasmic Reticulum Interactions of Adipocytes under Obesogenic Conditions
    Authors: López-Alcalá, J;Soler-Vázquez, MC;Tercero-Alcázar, C;Sánchez-Ceinos, J;Guzmán-Ruiz, R;Malagón, MM;Gordon, A;
    International journal of molecular sciences
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Lumican, an Exerkine, Protects against Skeletal Muscle Loss
    Authors: HJ Cho, YS Lee, DA Kim, SA Moon, SE Lee, SH Lee, JM Koh
    International Journal of Molecular Sciences, 2022-09-02;23(17):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Cleavage of proteoglycans, plasma proteins and the platelet-derived growth factor receptor in the hemorrhagic process induced by snake venom metalloproteinases
    Authors: AF Asega, MC Menezes, D Trevisan-S, D Cajado-Car, L Bertholim, AK Oliveira, A Zelanis, SMT Serrano
    Sci Rep, 2020-07-31;10(1):12912.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Bioassay
  4. Prolonged exposure to extracellular lumican restrains pancreatic adenocarcinoma growth
    Authors: X Li, Y Kang, D Roife, Y Lee, M Pratt, MR Perez, B Dai, EJ Koay, JB Fleming
    Oncogene, 2017-05-22;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Determination of the sites of tyrosine O-sulfation in peptides and proteins.
    Authors: Yu Y, Hoffhines AJ, Moore KL, Leary JA
    Nat. Methods, 2007-06-10;4(7):583-8.
    Species: Mouse
    Sample Types:
    Applications: Enzyme Assay Substrate

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reconstitution Buffers

Reviews for Recombinant Mouse Lumican Protein, CF

Average Rating: 4 (Based on 1 Review)

5 Star
0%
4 Star
100%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Recombinant Mouse Lumican Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Recombinant Mouse Lumican Protein, CF
By Anonymous on 08/21/2019
Application: In vitro bioactivity in cell culture