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Recombinant Human Lumican Protein, CF

Catalog #: 2846-LU
9 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
2846-LU-050

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Summary Product Datasheets Carrier Free Reconstitution Calculator Background Related Research Areas

Recombinant Human Lumican Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of BCE C/D‑1b bovine corneal endothelial cells. When 5 x 104 cells per well are added to Recombinant Human Lumican coated plates (10 μg/mL, 100 μL/well),  >35% will adhere after 50 minutes at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human Lumican protein
Gln19-Asn338, with a C-terminal 6-His tag
Accession #
P51884
N-terminal Sequence
Analysis
No results obtained: Gln19 predicted
Predicted Molecular Mass
37.5 kDa
SDS-PAGE
55-65 kDa, reducing conditions

Product Datasheets

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2846-LU

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2846-LU

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Background: Lumican

Lumican is a 40 kDa member of the family of small leucine-rich repeat proteoglycans (SLRPs) and the class II subfamily (1). Human Lumican is synthesized as a 338 amino acid (aa) precursor that contains an 18 aa signal sequence and a 320 aa mature chain (SwissProt #: P51884). The mature chain contains a negatively charged N-terminal domain containing sulfated tyrosine and disulfide bonds, 12 leucine-rich repeats (LRRs), four potential sites of N-linked glycosylation, and a carboxyl terminal domain containing two conserved cysteines (1). Mature human Lumican is 88%, 87%, and 70% aa identical to mature mouse, rat, and chick Lumican, respectively. SLRPs constitute an important fraction of noncollagenous extracellular matrix proteins (ECM) proteins (1, 2). Lumican is expressed in a variety of tissues, including skin, artery, lung, cornea, kidney, bone, aorta, and articular cartilage (1). Lumican’s role in vivo has been found using Lumican null mice. These mice have functional deficits including corneal opacity as well as skin and tendon fragility associated with disorganized and loosely packed collagen fibers
(1, 3-6). The abnormal connective tissue phenotype seen in the Lumican null mice shows the importance of the role of Lumican in collagen fibrillogenesis (1). In addition to the control of collagen fibril assembly, Lumican has been shown to play a role in the regulation of cell proliferation (7, 8), migration (8, 9), and adhesion (9). Lumican’s over-expression has been reported in carcinoid tumor, breast, colorectal, neuroendocrine, uterine cervical and pancreatic cancers (10).

References
  1. Nikitovic, D. et al. (2008) IUBMB Life 60:818.
  2. Blochberger, T.C. et al. (1992) J. Biol. Chem. 267:347.
  3. Chakravarti, S. et al. (1998) J. Cell Biol. 141:1277.
  4. Chakravarti, S. et al. (2000) Invest. Ophthalmol. Vis. Sci. 41:3365.
  5. Jepsen, K.J. et al. (2002) J. Biol. Chem. 277:35532.
  6. Chakravarti, S. et al. (2003) Invest. Ophthalmol. Vis. Sci. 44:2422.
  7. Vuillermoz, B. et al. (2004) Exp. Cell Res. 296:294.
  8. Nikitovic, D. et al. (2008) FEBS J. 275:350.
  9. D’Onofrio, M.F. et al. (2008) Biochem. Biophys. Res. Commun. 365:266.
  10. Ishiwata, T. et al. (2007) Oncol. Rep. 18:537.
Entrez Gene IDs
4060 (Human); 17022 (Mouse)
Alternate Names
KSPG lumican; LDC; LUM; lumican proteoglycan; Lumican; SLRR2D; SLRR2DLDCKeratan sulfate proteoglycan lumican

Citations for Recombinant Human Lumican Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Lumican Inhibits In Vivo Melanoma Metastasis by Altering Matrix-Effectors and Invadopodia Markers
    Authors: K Karamanou, M Franchi, I Proult, R Rivet, D Vynios, S Brézillon
    Cells, 2021-04-08;10(4):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Distinct effects of different matrix proteoglycans on collagen fibrillogenesis and cell-mediated collagen reorganization
    Authors: D Chen, LR Smith, G Khandekar, P Patel, CK Yu, K Zhang, CS Chen, L Han, RG Wells
    Sci Rep, 2020-11-04;10(1):19065.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  3. Cleavage of proteoglycans, plasma proteins and the platelet-derived growth factor receptor in the hemorrhagic process induced by snake venom metalloproteinases
    Authors: AF Asega, MC Menezes, D Trevisan-S, D Cajado-Car, L Bertholim, AK Oliveira, A Zelanis, SMT Serrano
    Sci Rep, 2020-07-31;10(1):12912.
    Species: Snake
    Sample Types: Protein
    Applications: Bioassay
  4. Prolonged exposure to extracellular lumican restrains pancreatic adenocarcinoma growth
    Authors: X Li, Y Kang, D Roife, Y Lee, M Pratt, MR Perez, B Dai, EJ Koay, JB Fleming
    Oncogene, 2017-05-22;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Lumican effectively regulates the estrogen receptors-associated functional properties of breast cancer cells, expression of matrix effectors and epithelial-to-mesenchymal transition
    Authors: K Karamanou, M Franchi, Z Piperigkou, C Perreau, FX Maquart, DH Vynios, S Brézillon
    Sci Rep, 2017-03-23;7(0):45138.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Transforming Growth Factor-? Limits Secretion of Lumican by Activated Stellate Cells within Primary Pancreatic Adenocarcinoma Tumors
    Clin Cancer Res, 2016-04-28;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Resistance of corneal RFUVA-cross-linked collagens and small leucine-rich proteoglycans to degradation by matrix metalloproteinases.
    Authors: Zhang Y, Mao X, Schwend T, Littlechild S, Conrad G
    Invest Ophthalmol Vis Sci, 2013-02-05;54(2):1014-25.
    Species: Human
    Sample Types: Protein
    Applications: Enzyme Assay
  8. Lumican, an extracellular matrix proteoglycan, is a novel requisite for hepatic fibrosis.
    Authors: Krishnan A, Li X, Kao W, Viker K, Butters K, Masuoka H, Knudsen B, Gores G, Charlton M
    Lab Invest, 2012-09-24;92(12):1712-25.
    Species: Rat
    Sample Types: Protein
    Applications: Bioassay
  9. Lumican induces human corneal epithelial cell migration and integrin expression via ERK 1/2 signaling.
    Authors: Seomun Y, Joo CK
    Biochem. Biophys. Res. Commun., 2008-05-12;372(1):221-5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Reconstitution Buffers

Reconstitution Buffer 1 (PBS)

RB01
3 Reviews

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Recombinant Human Lumican Protein, CF
By Christian Cassel on 09/19/2018
Application: In vitro bioactivity in cell culture