Recombinant Human VEGF/PlGF Heterodimer Protein
Recombinant Human VEGF/PlGF Heterodimer Protein Summary
Product Specifications
| Human VEGF (Ala27-Arg191) Accession # AAM03108 |
| Human PlGF (Ala21-Arg149) Accession # AAD30179 | |
| N-terminus | C-terminus |
Analysis
Product Datasheets
297-VP/CF (carrier free)
Discontinued Product
297-VP
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: VEGF/PlGF Heterodimer
The disulfide-linked homodimeric proteins vascular endothelial growth factor (VEGF) and placenta growth factor (PlGF) are both members of the VEGF family of growth factors. VEGF and PlGF proteins share primary structural as well as limited amino acid sequence homology with the A and B chains of PDGF. All eight cysteine residues involved in intra- and inter-chain disulfides are conserved among these growth factors. In their PDGF-like regions, VEGF and PlGF also share approximately 53% amino acid sequence similarity. E. coli-expressed monomers of PlGF and VEGF can be refolded in vitro to form PlGF/VEGF heterodimers. The purified recombinant VEGF/PlGF heterodimers and VEGF homodimers have potent mitogenic effects on endothelial cells. However, VEGF/PlGF heterodimers display approximately 50-fold less mitogenic activity than VEGF homodimers. In contrast, PlGF homodimers have little or no mitogenic effect on endothelial cells. The presence of natural VEGF/PlGF heterodimers in the conditioned media of a number of human tumor cell lines has been demonstrated using a VEGF/PlGF heterodimer specific ELISA.
Citations for Recombinant Human VEGF/PlGF Heterodimer Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Full Functional Knockout of Placental Growth Factor by Knockin with an Inactive Variant Able to Heterodimerize with VEGF-A
Authors: I Apicella, V Cicatiello, D Acampora, V Tarallo, S De Falco
Cell Rep, 2018-06-19;23(12):3635-3646.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Placental growth factor neutralising antibodies give limited anti-angiogenic effects in an in vitro organotypic angiogenesis model.
Authors: Brave SR, Eberlein C, Shibuya M, Wedge SR, Barry ST
Angiogenesis, 2010-10-16;13(4):337-47.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Microvascular patterning is controlled by fine-tuning the Akt signal.
Authors: Sun JF, Phung T, Shiojima I, Felske T, Upalakalin JN, Feng D, Kornaga T, Dor T, Dvorak AM, Walsh K, Benjamin LE
Proc. Natl. Acad. Sci. U.S.A., 2004-12-20;102(1):128-33.
Species: Bovine
Sample Types: Whole Cells
Applications: Bioassay -
A critical role of placental growth factor in the induction of inflammation and edema formation.
Authors: Oura H, Bertoncini J, Velasco P, Brown LF, Carmeliet P, Detmar M
Blood, 2002-09-05;101(2):560-7.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
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