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Recombinant Mouse Wnt-4 Protein

Catalog #: 475-WN
11 Citations Datasheet / COA / SDS

Discontinued Product

475-WN has been discontinued.
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Citations (11)
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Recombinant Mouse Wnt-4 Protein Summary

Product Specifications

Purity
>60%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 15-60 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse Wnt-4 protein
Ser23-Arg351
Accession #
NP_033549
N-terminal Sequence
Analysis
Ser23
Predicted Molecular Mass
36.6 kDa
SDS-PAGE
43 kDa, reducing conditions

Product Datasheets

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475-WN (with carrier)

Product Datasheet
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475-WN/CF (carrier free)

Discontinued Product

475-WN

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, EDTA and CHAPS with BSA as a carrier protein.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Background: Wnt-4

Wnt-4 is a 38 - 42 kDa member of the Wnt family of secreted glycoproteins, which act as short-range signaling molecules via Frizzled receptors and a cascade of intracellular signals in vertebrate embryogenesis (1 - 2). Mouse Wnt-4 is synthesized as a 351 amino acid (aa) precursor with a 22 aa signal sequence and a 329 aa mature chain. The mature chain contains two potential sites for N-linked glycosylation. Relative to other members of the Wnt family, Wnt-4 contains 83 conserved aa, including 21 cysteines (1). Mature mouse Wnt-4 shares 99% aa sequence identity with mature human and rat Wnt-4. Wnt-4 has been shown to play a critical role in the development of the reproductive system and in the formation of the kidneys, adrenals, pituitary gland, and mammary tissues (3 - 6). In the development of the reproductive system, Wnt-4 expression is down-regulated in the developing gonad after E11.5, although it persists in the developing ovary (2, 6). Targeted deletion of Wnt-4 results in masculinization of XX mice, with rudimentary development of the masculine internal (Wolffian) ducts and degeneration of the female (Mullerian) reproductive tract (2, 6). In addition to its involvement in urogenital development, Wnt-4 is also expressed in the perichondrium of the long bones (7), and promotes osteoblast differentiation (8). Wnt-4 may also be associated with abnormal proliferation in human breast tissue (9). In humans, mutations in Wnt-4 are the cause of SERKAL syndrome, a syndrome consisting of female to male sex reversal, renal, adrenal, and lung dysgenesis, and developmental defects (3), and Rokitansky-Kuster-Hauser syndrome, which is characterized by utero-vaginal atresia in otherwise phenotypically normal females with normal 46, XX karyotype (10).

References
  1. Gavin, B.J. et al. (1990) Genes Dev. 4:2319.
  2. Jordan, B.K. et al. (2001) Am. J. Hum. Genet. 68:1102.
  3. Mandel, H. et al. (2008) Am. J. Hum. Genet. 82:39.
  4. Bernard, P. and V.R. Harley (2007) Int. J. Biochem. Cell Biol. 39:31.
  5. Kuulasmaa, T. et al. (2008) Horm. Metab. Res. 40:668.
  6. Vainio, S. et al. (1999) Nature 397:405.
  7. Gao, X. (2004) Genes Dev. 18:2404.
  8. Chang, J. et al. (2007) J. Biol. Chem. 282:30938.
  9. Huguet, E.L. et al. (1994) Cancer Res. 54:2615.
  10. Philibert, P. et al. (2008) J. Clin. Endocrinol. Metab. 93:895.
Long Name
Wingless-type MMTV Integration Site Family, Member 4
Entrez Gene IDs
54361 (Human); 22417 (Mouse)
Alternate Names
protein Wnt-4; SERKAL; wingless-type MMTV integration site family, member 4; Wnt4; Wnt-4

Citations for Recombinant Mouse Wnt-4 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Wnt4 is heterogeneously activated in maturing beta-cells to control calcium signaling, metabolism and function
    Authors: K Katsumoto, S Yennek, C Chen, LFD Silva, S Traikov, D Sever, A Azad, J Shan, S Vainio, N Ninov, S Speier, A Grapin-Bot
    Nature Communications, 2022-10-21;13(1):6255.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  2. NR2F1 is a barrier to dissemination of early stage breast cancer cells
    Authors: C Rodriguez-, N Kale, MJ Carlini, N Shrivastav, AA Rodrigues, B Khalil, JJ Bravo-Cord, M Alexander, J Ji, Y Hong, F Behbod, MS Sosa
    Cancer Research, 2022-06-15;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  3. Wnt4 from the Niche Controls the Mechano-Properties and Quiescent State of Muscle Stem Cells
    Authors: S Eliazer, JM Muncie, J Christense, X Sun, RS D'Urso, VM Weaver, AS Brack
    Cell Stem Cell, 2019-09-05;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Tissue Culture
  4. Macrophages promote epithelial proliferation following infectious and non-infectious lung injury through a Trefoil factor 2-dependent mechanism
    Authors: LY Hung, D Sen, TK Oniskey, J Katzen, NA Cohen, AE Vaughan, W Nieves, A Urisman, MF Beers, MF Krummel, DR Herbert
    Mucosal Immunol, 2018-10-18;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  5. Morphological, molecular and FTIR spectroscopic analysis during the differentiation of kidney cells from pluripotent stem cells
    Authors: MM Mata-Miran, GJ Vazquez-Za, M Rojas-Lope, V Sanchez-Mo, DG Perez-Ishi, RJ Delgado-Ma
    Biol. Res., 2017-04-04;50(1):14.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Wnts contribute to neuromuscular junction formation through distinct signaling pathways
    Authors: J Messéant, J Ezan, P Delers, K Glebov, C Marchiol, F Lager, G Renault, F Tissir, M Montcouqui, N Sans, C Legay, L Strochlic
    Development, 2017-03-27;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  7. Early dissemination seeds metastasis in breast cancer
    Nature, 2016-12-14;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Identification of the genes regulated by Wnt-4, a critical signal for commitment of the ovary.
    Authors: Naillat F, Yan W, Karjalainen R, Liakhovitskaia A, Samoylenko A, Xu Q, Sun Z, Shen B, Medvinsky A, Quaggin S, Vainio S
    Exp Cell Res, 2015-01-30;332(2):163-78.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  9. Lgr4 gene regulates corpus luteum maturation through modulation of the WNT-mediated EGFR-ERK signaling pathway.
    Authors: Pan, Hongjie, Cui, Hengxian, Liu, Shijie, Qian, Yu, Wu, Haili, Li, Liang, Guan, Yuting, Guan, Xin, Zhang, Long, Fan, Heng-Yu, Ma, Yanlin, Li, Runsheng, Liu, Mingyao, Li, Dali
    Endocrinology, 2014-05-30;155(9):3624-37.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Endocardial to myocardial notch-wnt-bmp axis regulates early heart valve development.
    Authors: Wang, Yidong, Wu, Bingruo, Chamberlain, Alyssa A, Lui, Wendy, Koirala, Pratisth, Susztak, Katalin, Klein, Diana, Taylor, Verdon, Zhou, Bin
    PLoS ONE, 2013-04-01;8(4):e60244.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. An RNA interference-based screen of transcription factor genes identifies pathways necessary for sensory regeneration in the avian inner ear.
    Authors: Alvarado DM, Hawkins RD, Bashiardes S
    J. Neurosci., 2011-03-23;31(12):4535-43.
    Species: Chicken
    Sample Types: Whole Cells
    Applications: Bioassay

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