Anti- Adalimumab (Anti-Idiotype) Antibody

Detection of Adalimumab by Western Blot. Western blot shows Cetuximab, Rituximab Trastuzumab, and Adalimumab biosimilars. PVDF membrane was probed with 0.5 µg/mL of Anti- Adalimumab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB9616) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Adalimumab at approximately 240 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Specificity of Anti-Adalimumab (Anti-Idiotype) Antibody in ELISA Capture Assay. Antibody in ELISA Capture Assay. Biotinylated biosimilars were serially diluted and incubated with Anti-Adalimumab (Anti-Idiotype) Antibody (Catalog # MAB9616, green line) coated at 1 μg/mL on a 96-well polystyrene plate. No cross-reactivity with Rituximab (black line) or biotinylated-IgG control (orange line) was observed.

Specificity of Anti-Adalimumab (Anti-Idiotype) Antibody in Direct ELISA. A panel of biosimilars was coated at 1 μg/mL in 96-well polystyrene plates. Anti-Adalimumab (Anti-Idiotype) Antibody (Catalog # MAB9616, 333 ng/mL) detected Adalimumab. No cross-reactivity with Cetuximab, Rituximab, Trastuzumab, Brentuximab, Gemtuzumab, Ibritumomab, Tositumomab, Solanezumab, Teprotumumab, and Aducanumab was observed.

Anti-Adalimumab Bridging Assay. The anti-idiotype antibody (Catalog # MAB9616) coated at 4 μg/mL in a 96-well polystyrene plate binds the Adalimumab biosimilar Antibody. Biotinylated Anti-Adalimumab (Anti-Idiotype) Antibody used at 0.5 μg/mL detects the captured Adalimumab biosimilar relative to the concentration of the biosimilar. Non-related biosimilars do not bridge the anti-idotype antibodies.