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Anti- Daclizumab (Anti-Idiotype) Antibody

Catalog #: MAB10218 Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB10218-100
MAB10218-SP
Detection of Daclizumab by Western Blot.
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Anti- Daclizumab (Anti-Idiotype) Antibody Summary

Specificity
Detects Daclizumab in direct ELISAs and Western blots. In Western blots, no cross-reactivity with Basiliximab, Satumomab, Teplizumab, and Alemtuzumab is observed.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2498A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Full length Daclizumab
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Daclizumab antibody by Western Blot. View Larger

Detection of Daclizumab by Western Blot. Western blot shows Daclizumab, Basiliximab (negative control), Satumomab (negative control), Teplizumab (negative control), and Alemtuzumab (negative control) biosimilars. PVDF membrane was probed with 2 µg/mL of Anti- Daclizumab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB10218) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Daclizumab at approximately 250 kDa (as indicated). Human IgG is shown as a loading control. This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Daclizumab

The anti-Daclizumab antibody is an anti-Idiotype antibody raised against and selected for the antigenic determinants of the variable region of Daclizumab. The antibody has been tested against a broad range of other biosimilars to ensure specificity. This highly specialized anti-idiotypic antibody can be used in a variety of pharmacokinetic (PK) assay formats.

Alternate Names
Daclizumab

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FAQs

  1. What is an anti-idiotype antibody?

    • An anti-idiotype antibody is specific for the unique variable (antigen binding) region of another antibody.  We have anti-idiotype antibodies to commecially available therapeutic antibodies or  biosimilars.

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