Anti- Rituximab (Anti-Idiotype) PE-conjugated Antibody

Catalog #: FAB9630P Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
FAB9630P-100

Detection of Rituximab in PBMCs with CD19 costain cells by Flow Cytometry.

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All Rituximab Antibodies

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Anti- Rituximab (Anti-Idiotype) PE-conjugated Antibody Summary

Specificity

Detects Rituximab in Western blots in direct ELISAs. 100% cross-reactivity with Tositumomab and Ibritumomab (95% or higher sequence identity with Rituximab) and no cross-reactivity with Cetuximab, Trastuzumab, Brentuximab, Gemtuzumab, Adalimumab, Solanezumab, Teprotumumab, and Aducanumab is observed.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2260A

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Full-length Rituximab

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Rituximab in PBMCs with CD19 costain cells by Flow Cytometry. PBMCs with CD19 costain were stained with Mouse Anti-Human CD19 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB4867F) and either (A) Rabbit Anti-Rituximab (Anti-Idiotype) PE‑conjugated Monoclonal Antibody (Catalog # FAB9630P) or (B) Rabbit IgG PE-conjugated Antibody (Catalog # IC1051P). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Reconstitution

Reconstitution default not known

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Rituximab

The anti-Rituximab antibody is an anti-Idiotype antibody raised against and selected for the antigenic determinants of the variable region of Rituximab. The antibody has been tested against a broad range of other biosimilars to ensure specificity. This highly specialized anti-idiotypic antibody can be used in a variety of pharmacokinetic (PK) assay formats. In addition to Rituximab, the anti-Idiotype antibody also detects derivatives of Rituximab (Ibritumumab and Tositumumab).

Alternate Names

B lymphocyte antigen CD20; B lymphocyte surface antigen B1; Bp35; CD20; LEU 16; LEU16; Membrane spanning 4 domains subfamily A member 1; MS4A1; Rituximab