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Anti- Teprotumumab (Anti-Idiotype) Antibody

Catalog #: MAB96341 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB96341-100
MAB96341-SP
Detection of Anti-IGFLR-1 (Teprotumumab) on Human MCF-7 cells by flow cytometry.
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Anti- Teprotumumab (Anti-Idiotype) Antibody Summary

Specificity
Detects Teprotumumab in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 975547
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Full-length Teprotumumab
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Anti-IGFLR-1 (Teprotumumab) on Human MCF-7 cells antibody by Flow Cytometry. View Larger

Detection of Anti-IGFLR-1 (Teprotumumab) on Human MCF-7 cells by flow cytometry. MCF-7 human cell line was stained with Human anti-Human IGFLR-1 (Teprotumumab) Monoclonal Antibody (Catalog # MAB9718) then Anti-Teprotumumab (Anti-Idiotype) (Catalog # MAB96341, filled histogram) or Mouse IgG2Aisotype control antibody(Catalog # MAB003, open histogram) followed by PE-conjugated anti-mouse IgG secondary antibody (Catalog # F0102B).

Binding Inhibition Anti-IGFLR-1 (Teprotumumab) binding to MCF-7 Human Cell Line is Blocked by Anti-Teprotumumab (Anti-Idiotype) Antibody. View Larger

Anti-IGFLR-1 (Teprotumumab) binding to MCF-7 Human Cell Line is Blocked by Anti-Teprotumumab (Anti-Idiotype) Antibody. In a functional flow cytometry test, Biotinylated Anti-IGFLR-1 (Teprotumumab) (Catalog # FAB9718B) binds to MCF-7 human cell line (black dotted line). Binding is completely blocked (orange histogram) by 0.25 µg/mL of Anti-Teprotumumab (Anti-idiotype) Monoclonal Antibody (Catalog# MAB96341), but not by Normal Mouse IgG2A(Catalog # MAB003) (blue line). Cells were stained with APC-conjugated Streptavidin (Catalog # F0050).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Teprotumumab

The anti-Teprotumumab antibody is an anti-Idiotype antibody raised against and selected for the antigenic determinants of the variable region of Teprotumumab. The antibody has been tested against a broad range of other biosimilars to ensure specificity. This highly specialized anti-idiotypic antibody can be used in a variety of pharmacokinetic (PK) assay formats.

Alternate Names
Teprotumumab

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FAQs

  1. What is an anti-idiotype antibody?

    • An anti-idiotype antibody is specific for the unique variable (antigen binding) region of another antibody.  We have anti-idiotype antibodies to commecially available therapeutic antibodies or  biosimilars.

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