Bacterial Thermolysin Protein, CF Summary
Product Specifications
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
3097-ZN
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl, CaCl2 and Glycerol. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
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Background: Thermolysin
Thermolysin is a zinc protease that can be used to activate a number of proteases produced at R&D Systems (see product inserts for details). Its activity can be inhibited by phosphoramidon, 1,10-phenanthroline or EDTA.
| rhADAM8 | 1031-AD | 0.75 μg/mL | Phosphoramidon |
| rhEnterokinase | 1585-SE | 1.58 μg/mL | 1, 10-Phenanthroline |
| rhFactor VII | 2338-SE | 6.25 μg/mL | 1, 10-Phenanthroline |
| rhFactor XI | 2460-SE | 10 μg/mL | EDTA |
| rhHGF Activator | 1514-SE | 4.3 μg/mL | 1, 10-Phenanthroline |
| rhKallikrein 1 | 2337-SE | 1.0 μg/mL | 1, 10-Phenanthroline |
| rhKallikrein 3/PSA | 1344-SE | 1.0 μg/mL | 1, 10-Phenanthroline |
| rhKallikrein 4 | 1719-SE | 1.0 μg/mL | 1, 10-Phenanthroline |
| rhKallikrein 7 | 2624-SE | 10 μg/mL | EDTA |
| rhKallikrein 11 | 1595-SE | 10 μg/mL | EDTA |
| rhKallikrein 14 | 2626-SE | 10 μg/mL | EDTA |
| rmMast Cell Protease-11 | 2857-SE | 0.2 μg/mL | 1, 10-Phenanthroline |
| rhPlasma Kallikrein | 2497-SE | 10 μg/mL | EDTA |
| rmPlasma Kallikrein | 2498-SE | 10 μg/mL | EDTA |
| hThrombin | 2196-SE | 42 μg/mL | 1, 10-Phenanthroline |
| rmTryptase-5 | 2634-SE | 0.2 μg/mL | 1, 10-Phenanthroline |
| rmTryptase epsilon | 2059-SE | 0.3 μg/mL | 1, 10-Phenanthroline |
| rhTryptase beta 2 | 3796-SE | 0.1 μg/mL | 1, 10-Phenanthroline |
| rmFactor VII | 3178-PA | 1.0 μg/mL | 1, 10-Phenanthroline |
| rhProprotein Convertase 7 | 2984-SE | 2.0 μg/mL | 1, 10-Phenanthroline |
| rm Prss28 | 4808-SE | 0.1 μg/mL | 1, 10-Phenanthroline |
| rhFactor XIV/Protein C | 3349-SE | 2.3 μg/mL | 1, 10-Phenanthroline |
| rmFactor XIV/Protein C | 4885-SE | 5.0 μg/mL | 1, 10-Phenanthroline |
Citations for Bacterial Thermolysin Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Sodium/(calcium + potassium) exchanger NCKX4 optimizes KLK4 activity in the enamel matrix microenvironment to regulate ECM modeling
Authors: B Chan, IC Cheng, J Rozita, I Gorshteyn, Y Huang, I Shaffer, C Chang, W Li, J Lytton, P Den Besten, Y Zhang
Frontiers in Physiology, 2023-02-06;14(0):1116091.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
Crystal structures of human procathepsin H
Authors: Y Hao, W Purtha, C Cortesio, H Rui, Y Gu, H Chen, EA Sickmier, P Manzanillo, X Huang
PLoS ONE, 2018-07-25;13(7):e0200374.
Species: Human
Sample Types: Protein
Applications: Enzyme Assay -
N-glycosylation determines the abundance of the transient receptor potential channel TRPP2.
Authors: Hofherr A, Wagner C, Fedeles S, Somlo S, Kottgen M
J Biol Chem, 2014-04-09;289(21):14854-67.
Species: Mouse
Sample Types: Protein
Applications: Enzyme Assay -
Proteolytic activation cascade of the Netherton syndrome-defective protein, LEKTI, in the epidermis: implications for skin homeostasis.
Authors: Fortugno P, Bresciani A, Paolini C, Pazzagli C, El Hachem M, D'Alessio M, Zambruno G
J Invest Dermatol, 2011-06-23;131(11):2223-32.
Species: Human
Sample Types: Recombinant Protein
Applications: Enzyme Assay -
Inhibition of kallikrein-related peptidases by the serine protease inhibitor of Kazal-type 6.
Authors: Kantyka T, Fischer J, Wu Z, Declercq W, Reiss K, Schroder J, Meyer-Hoffert U
Peptides, 2011-03-23;32(6):1187-92.
Applications: Bioassay -
Targeting tumor endothelial marker 8 in the tumor vasculature of colorectal carcinomas in mice.
Authors: Fernando S, Fletcher BS
Cancer Res., 2009-06-15;69(12):5126-32.
Species: Mouse
Sample Types: Buffer
Applications: Enzyme Assay -
Active plasma kallikrein localizes to mast cells and regulates epithelial cell apoptosis, adipocyte differentiation, and stromal remodeling during mammary gland involution.
Authors: Lilla JN, Joshi RV, Craik CS, Werb Z
J. Biol. Chem., 2009-03-18;284(20):13792-803.
Species: Mouse
Sample Types: Buffer
Applications: Enzyme Assay -
The extracellular matrix protein fibronectin is a substrate for kallikrein 7.
Authors: Ramani VC, Haun RS
Biochem. Biophys. Res. Commun., 2008-03-14;369(4):1169-73.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
Activation of hepatocyte growth factor activator zymogen (pro-HGFA) by human kallikrein 1-related peptidases.
Authors: Mukai S, Fukushima T, Naka D, Tanaka H, Osada Y, Kataoka H
FEBS J., 2008-01-25;275(5):1003-17.
Species: Human
Sample Types: Protein
Applications: Enzyme Assay -
Expression and regulation of the metalloproteinase ADAM-8 during human neutrophil pathophysiological activation and its catalytic activity on L-selectin shedding.
Authors: Gomez-Gaviro M, Dominguez-Luis M, Canchado J, Calafat J, Janssen H, Lara-Pezzi E, Fourie A, Tugores A, Valenzuela-Fernandez A, Mollinedo F, Sanchez-Madrid F, Diaz-Gonzalez F
J. Immunol., 2007-06-15;178(12):8053-63.
Species: Human
Sample Types: Recombinant Protein
Applications: Enzyme Assay
FAQs
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Average Rating: 5 (Based on 3 Reviews)
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used to activate pro-KLK7 in an in vitro assay
Reason for Rating: Activity appears to be consistent and reliable. I am not measuring rates, otherwise may be able to judge specific activities given. Would use again.
