BDY FL VH032

Tocris Bioscience | Catalog # 7483

High-affinity VHL fluorescent probe for TR-FRET and FP assays
Tocris Bioscience

Key Product Details

Description

High-affinity VHL fluorescent probe for TR-FRET and FP assays

Wavelength

Excitation = 502 nm, Emission = 510 nm
View all Fluorescent Probes »

Product Description

BDY FL VH032 is a high-affinity VHL fluorescent probe (Kd = 3.01 nM) with application in time-resolved fluorescence resonance energy-transfer (TR-FRET) assays for high-throughput identification and characterization of VHL ligands. BDY FL VH032 consists of a derivative of von Hippel-Lindau (VHL) ligand VH 032 (Cat. No. 6911) and a fluorophore BDY FL (Cat. No. 5465) (with excitation peak at 504 nm and emission peak at 520 nm), joined by a polyethylene glycol (PEG) 4 linker. The BDY FL VH032 VHL TR-FRET signal is stable through the 90 - 300 min incubation time, and the TR-FRET binding assay is sensitive, selective and resistant to assay interference. BDY FL VH032 also exhibits high VHL affinity in a fluorescence polarization (FP) assay. Excitation maximum ~ 504 nm; emission maximum ~ 520 nm.

Licensing Information

Sold under license from St. Jude Children's Research Hospital

Optical Data

BDY FL VH032 Dye Spectra
Emission Color Green
Brightness
λabs 502 nm
λem 510 nm
Closest Laser line 488 nm
Application TR-FRET and FP Assays
Alternative For FAM-DEALAHypYIPMDDDFQLRSF, FAM-DEALA-Hyp-YIPD

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Product Specifications for BDY FL VH032

Molecular Weight

937.91

Formula

C46H62BF2N7O9S

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

(2S,4R)-1-((S)-2-(tert-Butyl)-21-(5,5-difluoro-7,9-dimethyl-5H-5λ4,6λ4-dipyrrolo[1,2-c:2',1'-f][1,3,2]diazaborinin-3-yl)-4,19-dioxo-6,9,12,15-tetraoxa-3,18-diazahenicosanoyl)-4-hydroxy-N-(4-(4-methylthiazol-5-yl)benzyl)pyrrolidine-2-carboxamide

CAS Number

2770675-66-4

InChI Key

RYFBKCMIPSQUPY-UOIAJAMCSA-N

SMILES

O=C(N[C@@H](C(C)(C)C)C(N1C[C@H](O)C[C@H]1C(NCC2=CC=C(C3=C(C)N=CS3)C=C2)=O)=O)COCCOCCOCCOCCNC(CCC4=CC=C5N4[B-]([N+]6=C(C)C=C(C)C6=C5)(F)F)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 9.38 10

Preparing Stock Solutions for BDY FL VH032

The following data is based on the product molecular weight 937.91.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 10.66 mL 53.31 mL 106.62 mL
0.5 mM 2.13 mL 10.66 mL 21.32 mL
1 mM 1.07 mL 5.33 mL 10.66 mL
5 mM 0.21 mL 1.07 mL 2.13 mL

Calculators

Calculate the mass, volume, or concentration required for a solution.

=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Calculate the dilution required to prepare a stock solution.

x
=
x

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background References

References are publications that support the biological activity of the product.

Product Documents for BDY FL VH032

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for BDY FL VH032

For research use only

Customer Reviews for BDY FL VH032

There are currently no reviews for this product. Be the first to review BDY FL VH032 and earn rewards!

Have you used BDY FL VH032?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card