Bovine IL-6 DuoSet ELISA

Catalog #: DY8190
15 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
DY8190

Ancillary Products Available

DY009B: DuoSet ELISA Ancillary Reagent Kit 3

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All IL-6 ELISAs

Product Details

Citations (15)

Supplemental Products

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Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Bovine IL-6 DuoSet ELISA Summary

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Sample Volume Required

100 µL

Assay Range

15.6 - 1,000 pg/mL

Sufficient Materials

For fifteen 96-well plates*

Specificity

Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and Recombinant . The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Product Features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits

Kit Content

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H₂O₂) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H₂SO₄ (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

N/A

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Bovine IL-6 ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-6

Interleukin 6 (IL-6) is a pleiotropic, alpha -helical, 22-28 kDa phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression (1-5). Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties (7-10). Cells known to express IL-6 include CD8+ T cells, fibroblasts, synoviocytes, adipocytes, osteoblasts, megakaryocytes, endothelial cells (under the influence of endothelins), sympathetic neurons, cerebral cortex neurons, adrenal medulla chromaffin cells, retinal pigment cells, mast cells, keratinocytes, Langerhans cells, fetal and adult astrocytes, neutrophils, monocytes, eosinophils, colonic epithelial cells, B1 B cells and pancreatic islet beta cells (2, 11-33). IL-6 production is generally correlated with cell activation and is normally kept in control by glucocorticoids, catecholamines, and secondary sex steroids (2).

IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha, triggering IL-6 R alpha association with gp130 and gp130 dimerization (39). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (40). Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage (5). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130- expressing cells that lack cell surface IL-6 R alpha (5). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes (2, 5). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor (5, 41).

IL-6, along with TNF-alpha and IL-1, drives the acute inflammatory response. IL-6 is almost solely responsible for fever and the acute phase response in the liver, and it is important in the transition from acute inflammation to either acquired immunity or chronic inflammatory disease (1-5). When dysregulated, it contributes to chronic inflammation in conditions such as obesity, insulin resistance, inflammatory bowel disease, arthritis, and sepsis (2, 5). IL-6 modulates bone resorption and is a major effector of inflammatory joint destruction in rheumatoid arthritis through its promotion of Th17 cell development and activity (1). It contributes to atherosclerotic plaque development and destabilization as well as the development of inflammation-associated carcinogenesis (1, 2). IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise (2). In addition, it enhances hematopoietic stem cell proliferation and the differentiation of memory B cells and plasma cells (42).

Long Name:

Interleukin 6

Entrez Gene IDs:

3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 102138971 (Cynomolgus Monkey); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)

Alternate Names:

B-cell differentiation factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CTL differentiation factor ; HSF; hybridoma growth factor; IFNB2; IFN-beta-2; IL6; IL-6; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A

Citations for Bovine IL-6 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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Maternal supplementation with n-3 fatty acids affects placental lipid metabolism, inflammation, oxidative stress, the endocannabinoid system, and the neonate cytokine concentrations in dairy cows
Authors: Dos Santos Silva, P;Kra, G;Butenko, Y;Daddam, JR;Levin, Y;Zachut, M;
Journal of animal science and biotechnology
Species: Bovine
Sample Types: Plasma
Applications: ELISA
Cytokine production by bovine adipose tissue stromal vascular fraction cells upon Neospora caninum stimulation
Authors: Oliveira, BM;Sidónio, B;Correia, A;Pinto, A;Azevedo, MM;Sampaio, P;Ferreira, PG;Vilanova, M;Teixeira, L;
Scientific reports
Species: Bovine
Sample Types: Cell Culture Supernates
Differential modulation of innate immune response by lipopolysaccharide of Leptospira
Authors: Varma, VP;Bankala, R;Kumar, A;Gawai, S;Faisal, SM;
Open biology
Species: Bovine
Sample Types: Cell Culture Supernates
Anti-inflammatory effects of the prostaglandin D2/prostaglandin DP1 receptor and lipocalin-type prostaglandin D2 synthase/prostaglandin D2 pathways in bacteria-induced bovine endometrial tissue
Authors: J Wu, F Bai, W Mao, B Liu, X Yang, J Zhang, T Li, G Borjigin, J Cao
Veterinary research, 2022-11-26;53(1):98.
Species: Bovine
Sample Types: Tissue Homogenates
Intramammary treatment using allogeneic pure platelet-rich plasma in cows with subclinical mastitis caused by Gram-positive bacteria
Authors: PC Duque-Madr, J Velasco-Bo, A Ceballos-M, C López, JU Carmona
Scientific Reports, 2021-12-09;11(1):23737.
Species: Bovine
Sample Types: Milk
Early inflammatory events of mastitis-a pilot study with the isolated perfused bovine udder
Authors: KS Brand, V Filor, W Bäumer
Bmc Veterinary Research, 2021-11-19;17(1):356.
Species: Bovine
Sample Types: Tissue Homogenates
Colonization and local host response following intramammary Staphylococcus chromogenes challenge in dry cows
Authors: L Beuckelaer, A De Vissche, FN Souza, E Meyer, F Haesebrouc, S Piepers, S De Vlieghe
Veterinary research, 2021-10-28;52(1):137.
Species: Bovine
Sample Types: Whey
Chronic heat stress delays immune system development and alters serotonin signaling in pre-weaned dairy calves
Authors: MG Marrero, B Dado-Senn, SL Field, G Yang, JP Driver, J Laporta
PLoS ONE, 2021-06-04;16(6):e0252474.
Species: Bovine
Sample Types: Cell Culture Supernates
Metformin activated AMPK signaling contributes to the alleviation of LPS-induced inflammatory responses in bovine mammary epithelial cells
Authors: T Xu, X Wu, X Lu, Y Liang, Y Mao, JJ Loor, Z Yang
Bmc Veterinary Research, 2021-03-01;17(1):97.
Species: Bovine
Sample Types: Cell Culture Supernates
Non-specific protection from respiratory tract infections in cattle generated by intranasal administration of an innate immune stimulant
Authors: W Wheat, L Chow, V Rozo, J Herman, K Still Broo, A Colbath, R Hunter, S Dow
PLoS ONE, 2020-06-25;15(6):e0235422.
Species: Bovine
Sample Types: Cell Culture Supernates

Effects of omega-3 fatty acids on immune, health and growth variables in veal calves
Authors: C Masmeijer, K van Leenen, L De Cremer, P Deprez, E Cox, B Devriendt, B Pardon
Prev. Vet. Med., 2020-04-07;179(0):104979.
Species: Bovine
Sample Types: Cell Culture Supernates

TLR2/4 promotes PGE2 production to increase tissue damage in Escherichia coli-infected bovine endometrial explants via MyD88/p38 MAPK pathway
Authors: T Li, L Hai, B Liu, W Mao, K Liu, Yuan Shen, Q Li, Y Guo, Y Jia, H Bao, J Cao
Theriogenology, 2020-04-07;152(0):129-138.
Species: Bovine
Sample Types: Cell Culture Superantes

LP induced/mediated PGE2 synthesis through activation of the ERK/NF-?B pathway contributes to inflammatory damage triggered by Escherichia coli-infection in bovine endometrial tissue
Authors: T Li, W Mao, B Liu, R Gao, S Zhang, J Wu, C Fu, Y Deng, K Liu, Y Shen, J Cao
Vet. Microbiol., 2019-03-08;232(0):96-104.
Species: Bovine
Sample Types: Tissue Culture Supernates

Jugular arginine infusion relieves lipopolysaccharide-triggered inflammatory stress and improves immunity status of lactating dairy cows
Authors: FF Zhao, TY Wu, HR Wang, LY Ding, G Ahmed, HW Li, W Tian, YZ Shen
J. Dairy Sci., 2018-04-05;0(0):.
Species: Bovine
Sample Types: Serum

Regulation of cytokine gene expression by orosomucoid in neonatal swine adipose tissue
Authors: TG Ramsay, MJ Stoll, le A Blomberg, TJ Caperna
J Anim Sci Biotechnol, 2016-04-14;7(0):25.
Species: Bovine
Sample Types: Cell Culture Supernates