Bromodeoxyuridine/BrdU Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Bromodeoxyuridine/BrdU in MCF‑7 Human Cell Line. Bromodeoxyuridine/BrdU was detected in immersion fixed MCF-7 human breast cancer cell line stimulated with BrdU using Mouse Anti-Bromodeoxyuridine/ BrdU Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB7225) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Bromodeoxyuridine/BrdU in Human Breast Cancer Tissue. Bromodeoxyuridine/BrdU was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Bromodeoxyuridine/BrdU Monoclonal Antibody (Catalog # MAB7225) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Bromodeoxyuridine/BrdU in Human Testis. Bromodeoxyuridine/BrdU was detected in immersion fixed paraffin-embedded sections of human testis using Mouse Anti-Bromodeoxyuridine/BrdU Monoclonal Antibody (Catalog # MAB7225) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Detection of Bromodeoxyuridine/BrdU in Human PBMCs by Flow Cytometry Human peripheral blood mononuclear cells (PBMCs) were treated overnight with 50 ng/mL PMA, 500 ng/mL Ionomycin, and 30 µg/mL BrdU, then stained with Mouse Anti-Bromodeoxyuridine/BrdU Monoclonal Antibody (Catalog # MAB7225, filled histogram) or isotype control antibody (Catalog # MAB003, open histo-gram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with cold, 70% ethanol for 5 minutes, DNA was denatured with 1.5M HCl for 30 minutes, and then cells were permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Bromodeoxyuridine/BrdU
Bromodeoxyuridine (BrdU) is a nucleoside analog that is incorporated into DNA in place of thymidine. The detection of newly synthesized DNA containing BrdU is a commonly used measure of cell proliferation and progression through S phase of the cell cycle.
Product Datasheets
Citations for Bromodeoxyuridine/BrdU Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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A Dual Role for Death Receptor 5 in Regulating Cardiac Fibroblast Function
Authors: Miles A. Tanner, Laurel A. Grisanti
Frontiers in Cardiovascular Medicine
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Dihydroartemisinin regulates immune cell heterogeneity by triggering a cascade reaction of CDK and MAPK phosphorylation
Authors: Qilong Li, Quan Yuan, Ning Jiang, Yiwei Zhang, Ziwei Su, Lei Lv et al.
Signal Transduction and Targeted Therapy
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TAGLN2 induces resistance signature ISGs by activating AKT-YBX1 signal with dual pathways and mediates the IFN-related DNA damage resistance in gastric cancer
Authors: Zhuo, H;Hou, J;Hong, Z;Yu, S;Peng, H;Zhang, L;Xie, W;Hong, X;
Cell death & disease
Species: Human
Sample Types: Whole Cells
Applications: Immunocytochemistry -
Developmental modeling of hepatogenesis using obese iPSCs-hepatocyte differentiation uncovers pathological features
Authors: DS Varghese, TT Alawathugo, MA Sheikh, AK Challagand, BS Emerald, SA Ansari
Cell Death & Disease, 2022-08-01;13(8):670.
Species: Human
Sample Types: Whole Cells
Applications: IF -
Deciphering the Molecular Mechanism of Spontaneous Senescence in Primary Epithelial Ovarian Cancer Cells
Authors: M Paku?a, E Ma?y, P Uruski, A Witucka, M Bogucka, N Jaroszewsk, N Makowska, A Niklas, R Moszy?ski, S Sajdak, A Tykarski, J Miku?a-Pie, K Ksi??ek
Cancers (Basel), 2020-01-27;12(2):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
PDGFRbeta expression and function in fibroblasts derived from pluripotent cells is linked to DNA demethylation.
J. Cell. Sci., 2012-02-17;125(0):2276-87.
Species: Human
Sample Types: Whole Cells
Applications: ICC
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