C. botulinum BoNT-C1 Heavy Chain Antibody

Catalog #: MAB5425 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5425
MAB5425-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

C. botulinum BoNT-C1 Heavy Chain Antibody Summary

Species Reactivity

C. botulinum

Specificity

Detects C. botulinum BoNT-C1 Heavy Chain in direct ELISAs. In direct ELISAs, no cross-reactivity with the Light Chains of BoNT-A, -B, -C1, -D, -E, -F, -G, or Heavy Chains of BoNT-D or -G is observed.

Source

Monoclonal Mouse IgG₁ Clone # 580223

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant C. botulinum BoNT-C1 Heavy Chain
Asn866-Glu1291
Accession # P18640

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant C. botulinum BoNT-C1 Heavy Chain

Immunoprecipitation

25 µg/mL

Cell lysates spiked with recombinant C. botulinum BoNT-C1 Heavy Chain, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BoNT-C1 Heavy Chain

BoNT-C1 (Botulinum neurotoxin C1 [heavy chain]) is a 100 kDa C-terminal fragment of the C1 Clostridum botulinum neurotoxin (holo)precursor. It constitutes a nonenzymatic part of a Zn²⁺-dependent M27 family endoprotease that potently blocks neurosecretion. The inactive 1291 amino acid (aa), 150 kDa C1 precursor is cleaved between Lys449Thr450 to generate a heterodimeric toxin held together by a preexisting interchain disulfide bond between Cys437 and Cys453. The 100 kDa C-terminus (heavy chain) acts as a ligand for neuronal membrane ecto-acceptors, inducing its translocation and internalization. The 50 kDa N-terminal light chain acts as an intracellular peptidase that cleaves SNAP-25 and syntaxin. This is suggested to block noradrenalin release from a readily releasable pool of vesicles. Over aa 866-1291, the C1 heavy chain shares less than 40% aa identity with the D-type neurotoxin heavy chain.

Long Name

Botulinum Neurotoxin Type C1 Heavy Chain

Entrez Gene IDs

10523893 (C. botulinum)

Alternate Names

BoNTC1 Heavy Chain; BoNT-C1 Heavy Chain; DcD