Chicken Netrin-2 Antibody

Catalog #: MAB127 Datasheet / COA / SDS

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MAB127
MAB127-SP

All Netrin-2 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Chicken Netrin-2 Antibody Summary

Species Reactivity

Chicken

Specificity

Detects chicken Netrin-2 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 74506

Purification

Protein A or G purified from ascites

Immunogen

Mouse myeloma cell line NS0-derived recombinant chicken Netrin-2
Ala16-Pro581
Accession # Q90923

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Chicken Netrin‑2 (Catalog # 127-N2) under non-reducing conditions only

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Netrin-2

Netrins are laminin-like axon guidance molecules that are conserved among C. elegans, Drosophila, and vertebrates. The chicken Netrin-2 cDNA encodes a precursor protein that has structural similarity to the N-terminus of B2 or the gamma -chain of laminin. The mature Netrin-2 protein has 581 amino acid (aa) which contains a 245 aa domain VI, a 169 aa domain V with three cysteine/glycine rich EGF repeats and a unique 152 aa domain C that enriches in basic aa residues and contains an RGD motif. Chicken Netrin-2 shares 72% aa identity with chicken Netrin-1, 51% aa identity with C. elegans UNC-6 protein and 57% aa identity with human NTN2L (Netrin‑2‑like). Although only two chicken netrins are known, more mammalian netrins are known. Netrin-2 is expressed in the chick brain and spinal cord from E6 through to the adult. In addition, expression of Netrin‑2 was observed at various stages in several mesodermally and endodermally derived tissues, including lung, gut, ovary, testes and spleen. Netrins act to both attract and repel the growing axons of a broad range of neuronal cell types during development. These actions are mediated by specific receptor complexes containing either the DCC (colorectal carcinoma) or neogenin, in the case of attractant, or UNC-5-related proteins, in the case of repellent.

References

Serafini, T. et al. (1994) Cell 78:409.
Kennedy, T.E. et al. (1994) Cell 78:425.
Livesey, F.J. (1999) Cell. Mol. Life Sci. 56:62.

Entrez Gene IDs

4917 (Human); 396387 (Chicken)

Alternate Names

Netrin2; Netrin-2; NTN2L

Product Datasheets

Product Datasheet

COA

SDS

Product Specific Notices

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 5,565,331, 6,096,866, 6,017,714, 6,309,638, 6,670,451, and other U.S. and international patents pending.