Chicken RGM-A Antibody

Catalog # Availability Size / Price Qty
MAB2010
MAB2010-SP
Product Details
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Chicken RGM-A Antibody Summary

Species Reactivity
Chicken
Specificity
Detects chicken RGM‑A in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human RGM-A or recombinant mouse RGM-A is observed.
Source
Monoclonal Mouse IgG2B Clone # 292012
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant chicken RGM-A
Pro150-Asn404 (Asn404Asp), predicted
Accession # Q8JG54
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Chicken Repulsive Guidance Molecule A/RGM-A

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RGM-A

RGM-A is the founding member of the RGM family of glycosylphosphatidylinositol (GPI)- anchored axon guidance proteins. It is a disulfide-linked dimer of the N‑terminal and C-terminal RGD-containing segments. RGM-A binds with high affinity to Neogenin.

Long Name
Repulsive Guidance Molecule A
Entrez Gene IDs
56963 (Human); 244058 (Mouse)
Alternate Names
RGM domain family member A; RGM domain family, member A; RGMA; RGM-A; RGMrepulsive guidance molecule A

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