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CHIR 99021

Catalog #: TB4423-GMP
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
TB4423-GMP/10
CHIR 99021 | CAS No. 252917-06-9 | GSK-3 Inhibitors
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Description: CHIR 99021 synthesized to cGMP guidelines

Chemical Name: 6-[[2-[[4-(2,4-Dichlorophenyl)-5-(5-methyl-1H-imidazol-2-yl)-2-pyrimidinyl]amino]ethyl]amino]-3-pyridinecarbonitrile

Purity: ≥99%

Product Details
Citations (1)
Reviews
Biological Activity Scientific Data Technical Data Product Datasheets Calculators

Biological Activity

CHIR 99021 synthesized to cGMP guidelines. Suitable for use as an ancillary reagent in the further manufacturing of cell therapies.

For more information about how CHIR 99021 may be used, please see our protocols: Transdifferentiating Fibroblasts into Neurons, Culturing Transcriptomically Distinct Pluripotent mESCs, Converting Fibroblasts into Cardiomyocytes (9C Cocktail), Human iPSC-derived Myoblasts, Reprogramming MEFs into Skeletal Muscle Progenitors, Highly Efficient Generation of CiPSCs from MEFs, Generation of Kidney Organoids from hPSCs.

Research use only CHIR 99021 also available.

Scientific Data

N/A Heart organoids formed from iPSCs using CHIR 99021 View Larger

iPSC-derived Human Heart-forming Organoids Human iPSCs were embedded in Cultrex™ UltiMatrix RGF Basement Membrane Extract (R&D Systems, Catalog # BME001-05) and differentiated with CHIR 99021 (Tocris Bioscience, Catalog # TB4423-GMP) and IWP 2 (Tocris Bioscience, Catalog # 3533) into heart-forming organoids based on a protocol from Drakhlis, L. et al. (2021) Nat. Biotechnol. 39:737. Successful differentiation was demonstrated by staining the cells with A) a Rabbit Anti-Human Cardiac Troponin T Antigen Affinity-purified Polyclonal Antibody (green; Novus Biologicals, Catalog # NBP3-13391) to detect cardiomyocytes and a Goat Anti-Human CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (red; R&D Systems, Catalog # AF3628) to detect endothelial cells. The cells were counterstained with DAPI (blue; Tocris Bioscience, Catalog # 5748). As formation of early embryonic heart anlagen is associated with nascent foregut endoderm development, cells were also stained with B) a Goat Anti-Human SOX2 Antigen Affinity-purified Polyclonal Antibody (red; R&D Systems, Catalog # AF2018) and a Mouse Anti-Human HNF-4 alpha/NR2A1 Monoclonal Antibody (green; R&D Systems, Catalog # PP-K9218-00) to detect anterior and posterior foregut endoderm cells, respectively. The cells were counterstained with DAPI (blue; Tocris Bioscience, Catalog # 5748).

Current Good Manufacturing Practice (cGMP) is a harmonized set of guidelines that when implemented will ensure safe, reliable, consistent and quality-assured products. The Tocris range of cGMP small molecules is manufactured following relevant sections of ICH Q7 guidelines. Our highly skilled team has full control over the cGMP process, meaning we can ensure: a quality-assured manufacturing system, batch-to-batch consistency, consistent supply, traceability from starting material to final product, and animal-free production. Find out more about the Tocris range of cGMP compounds.

Technical Data

M.Wt:
465.34
Formula:
C22H18Cl2N8
Purity:
≥99%
Storage:
Store at -20°C(±5°C)
CAS No:
252917-06-9

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
This product is intended for research use or further manufacturing only.

Product Datasheets

Certificate of Analysis is currently unavailable on-line. Please contact Customer Service
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Citation for CHIR 99021

The citations listed below are publications that use Tocris products. Selected citations for CHIR 99021 include:

1 Citation: Showing 1 - 1

  1. Constructing an Isogenic 3D Human Nephrogenic Progenitor Cell Model Composed of Endothelial, Mesenchymal, and SIX2-Positive Renal Progenitor Cells.
    Authors: Nguyen Et al.
    Stem Cells Int  2019;2019:3298432

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