CometAssay 96-well Electrophoresis Starter Kit
CometAssay 96-well Electrophoresis Starter Kit Summary
The complete and standardized system for the comet assay includes a 96 test sample Reagent Kit (1 x 96 well slide), Alkaline Control Cells, and the CometAssay Electrophoresis System II for consistent and reproducible results. Each lot of the Alkaline Control Cells, reagents and CometSlides are tested and qualified for use in the CometAssay ES II.What is the CometAssay?
The Comet assay, or single cell gel electrophoresis assay, provides a simple and effective method for evaluating DNA damage in cells. The principle of the assay is based upon the ability of denatured, cleaved DNA fragments to migrate out of the nucleoid under the influence of an electric field, whereas undamaged DNA migrates slower and remains within the confines of the nucleoid when a current is applied. Evaluation of the DNA “comet” tail shape and migration pattern allows for assessment of DNA damage. The general Comet assay protocol includes first immobilizing cells in a bed of low melting point agarose, gently lysing the cells, treating cells with neutral or alkali solutions to unwind and denature the DNA as well as hydrolyze sites of damage. DNA is then subjected to electrophoresis and stained with a fluorescent DNA intercalating dye.
Why Use the CometAssay 96-well Electrophoresis Starter Kit?
CometAssay 96-well Electrophoresisis Starter Kit is designed to eliminate known causes of comet assay variability and provide a highthroughput method for assessing DNA damage. It provides an exclusive 96-well CometSlide that is specially treated to promote adherence of low melting point agarose. This eliminates the time consuming and unreliable traditional method of preparing base layers of agarose. The use of 96-well CometSlide High Throughput shortens the overall assay time and allows for the rapid and reliable analysis of large numbers of samples.
The complete and standardized system for the comet assay includes a 96 test sample Reagent Kit (1 x 96 well slide), Alkaline Control Cells, and the CometAssay Electrophoresis System II for consistent and reproducible results. Each lot of the Alkaline Control Cells, reagents and CometSlides are tested and qualified for use in the CometAssay ES II.
Kit Contents
• CometAssay Lysis Solution
• CometAssay LMAgarose
• 200 mM EDTA, pH 10
• 96-well CometSlide
• CometAssay Alkaline Control Cells
• CometAssay Electrophoresis System II
Specifications
Limitations
For research use only. Not for diagnostic use.
Product Datasheets
Citations for CometAssay 96-well Electrophoresis Starter Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Neurotoxicological profile of the hallucinogenic compound 25I-NBOMe
Authors: M Herian, A Wojtas, M Ma?kowiak, A Wawrzczak-, A Solarz, A Bysiek, K Madej, K Go?embiows
Scientific Reports, 2022-02-21;12(1):2939. 2022-02-21
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Cyclometalated Ru(II)-isoquinoline complexes overcome cisplatin resistance of A549/DDP cells by downregulation of Nrf2 via Akt/GSK-3beta/Fyn pathway
Authors: L Chen, J Wang, X Cai, S Chen, J Zhang, B Li, W Chen, X Guo, H Luo, J Chen
Bioorganic chemistry, 2021-11-25;119(0):105516. 2021-11-25
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X-ray repair cross-complementing protein 1 (XRCC1) loss promotes beta-lapachone -induced apoptosis in pancreatic cancer cells
Authors: Y Zheng, H Zhang, Y Guo, Y Chen, H Chen, Y Liu
BMC Cancer, 2021-11-17;21(1):1234. 2021-11-17
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Two-Step Approach Using Degradable Magnesium to Inhibit Surface Biofilm and Subsequently Kill Planktonic Bacteria
Authors: PC Wong, RY Wang, LS Lu, WR Wang, JS Jang, JL Wu, TY Su, LH Chang
Biomedicines, 2021-11-12;9(11):. 2021-11-12
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Disrupted mitochondrial homeostasis coupled with mitotic arrest generates antineoplastic oxidative stress
Authors: X Hao, W Bu, G Lv, L Xu, D Hou, J Wang, X Liu, T Yang, X Zhang, Q Liu, Y Gong, C Shao
Oncogene, 2021-11-12;0(0):. 2021-11-12
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NAMPT-derived NAD+ fuels PARP1 to promote skin inflammation through parthanatos cell death
Authors: FJ Martínez-M, J Cantón-San, FJ Martínez-N, I Cabas, I Martínez-V, J Armistead, J Hatzold, A López-Muño, T Martínez-M, R Corbalán-V, J Lacal, M Hammerschm, JC García-Bor, A García-Aya, ML Cayuela, AB Pérez-Oliv, D García-Mor, V Mulero
PloS Biology, 2021-11-08;19(11):e3001455. 2021-11-08
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Persistent DNA damage triggers activation of the integrated stress response to promote cell survival under nutrient restriction
Authors: E Clementi, L Inglin, E Beebe, C Gsell, Z Garajova, E Markkanen
BMC Biol., 2020-03-30;18(1):36. 2020-03-30
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Norepinephrine-Induced DNA Damage in Ovarian Cancer Cells
Authors: R Lamboy-Car, C Ortiz-Sanc, A Acevedo-Sa, J Matta, A N A Montei, G N Armaiz-P
Int J Mol Sci, 2020-03-24;21(6):. 2020-03-24
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FBH1 promotes DNA double-strand breakage and apoptosis in response to DNA replication stress.
Authors: Jeong Y, Rossi M, Cermak L, Saraf A, Florens L, Washburn M, Sung P, Schildkraut C, Pagano M
J Cell Biol, 2013-01-14;200(2):141-9. 2013-01-14
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