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CometAssay Alkaline Control Cells

Catalog #: 4256-010-CC
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
4256-010-CC
Product Details
Citations (3)
FAQs
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Summary Product Datasheets

CometAssay Alkaline Control Cells Summary

Cryopreserved cell preparations contain different levels of DNA damage to be used as controls when performing the comet assay.
 

Key Benefits

• Provides a range of DNA damage for comparison with statistically distinct populations.
• Allows for electrophoresis method to be standardized between individual users.
• Standardized with the CometAssay Electrophoresis System
• Cell populations remain stable in liquid nitrogen for long term storage.

What are CometAssay Alkaline Control Cells?

These cryopreserved cell preparations contain different levels of DNA damage to be used as controls when performing the comet assay. Cells are provided at 5 x 10^5 cells/mL. A healthy control cell population (C0) is treated with a DNA damaging agent to increase the amount of damage in populations C1, C2 and C3, respectively. Etoposide is the damaging agent used for Alkaline Control Cells (CC0-CC3).

Specifications

Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store in liquid nitrogen for up to 12 months from the date of receipt.
Species
N/A

Limitations

For research use only. Not for diagnostic use.

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Citations for CometAssay Alkaline Control Cells

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Genetic and environmental risk for lymphoma in boxer dogs
    Authors: K Craun, J Ekena, J Sacco, T Jiang, A Motsinger-, LA Trepanier
    J. Vet. Intern. Med., 2020-07-15;0(0):.  2020-07-15
  2. The existence of MTH1-independent 8-oxodGTPase activity in cancer cells as a compensatory mechanism against on-target effects of MTH1 inhibitors
    Authors: GJ Samaranaya, CI Troccoli, L Zhang, M Huynh, CJ Jayaraj, D Ji, L McPherson, Y Onishi, DM Nguyen, DJ Robbins, M Karbaschi, MS Cooke, A Barrientos, ET Kool, P Rai
    Mol. Cancer Ther., 2019-11-19;0(0):.  2019-11-19
  3. Identification of potentially cytotoxic lesions induced by UVA photoactivation of DNA 4-thiothymidine in human cells.
    Authors: Reelfs O, Macpherson P, Ren X, Xu Y, Karran P, Young A
    Nucleic Acids Res, 2011-09-02;39(22):9620-32.  2011-09-02

FAQs

  1. What is the purpose of the CometAssay® Control Cells?

    • The CometAssay Control Cells are designed to help standardize and compare alkaline and neutral electrophoresis methods between individual users and laboratories. The control cells contain known levels of damage.

  2. How should the CometAssay® Control Cells be stored?

    • The CometAssay Control Cells should be stored in liquid nitrogen. To avoid the accumulation of damage due to freeze thaw cycles, the CometAssay Control Cells should be thawed and frozen in working aliquots. Refer to the CometAssay Control Cells literature for specific instructions.

  3. What cell line is used to generate the CometAssay® Control Cells?

    • CometAssay Control Cells were prepared using an immortalized human lymphocyte line.

  4. What DNA damaging agent was used to create the CometAssay® Control Cells?

    • A healthy control cell population (C0) is treated with a DNA damaging agent to increase the amount of damage in populations C1, C2 and C3, respectively. Etoposide is the damaging agent used for Alkaline Control Cells (CC0-CC3) and Bleomycin is the damaging agent used for Neutral Control Cells (NC0-NC3).

  5. Can the Alkaline CometAssay® Control Cells be used for the Neutral CometAssay?

    • The Alkaline CometAssay Control Cells were qualified for the Alkaline CometAssay while the Neutral CometAssay Control Cells were qualified for the Neutral CometAssay.

  6. Why is it necessary to rinse the CometAssay® Control Cells in PBS before adding to LMAgarose?

    • The CometAssay Control Cells must be rinsed in PBS to eliminate media and storage factors that reduce adherence of LMAgarose to the slide. PBS without magnesium should be used, as magnesium stimulates nucleases.

  7. Why don't I see a visible cell pellet after centrifugation of the CometAssay® Control Cells?

    • A pellet may not be visible after centrifugation due to the low number of cells.

  9. Why are there comet tails in the healthy CometAssay® Control Cell population?

    • The CometAssay Control Cells were prepared from an asynchronous cell population with cells at all stages of the cell cycle. Some percentage of the population will be undergoing DNA replication and may have strand breaks at replication forks.

      Improper storage may also lead to DNA damage. Do not store CometAssay Control Cells at -80°C. Store in aliquots in liquid nitrogen.

  10. Do control cells, Catalog # 4256-010-CC, just need to be put on a slide then stained? Or do they need to be ran through the entire Catalog # 4250-050-K protocol to see comets?

    • The control cells, Catalog # 4256-010-CC, need to be run through the entire protocol for Catalog # 4250-050-K in order to see comets.

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