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Cultrex 3-D Spheroid Colorimetric Assay, 96-well

Catalog #: 3511-096-K
6 Citations 2 Reviews Datasheet / COA / SDS
Colorimetric Proliferation/Viability Assay

Discontinued Product

3511-096-K has been discontinued.
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Product Details
Citations (6)
FAQs
Reviews (2)
Summary Product Datasheets

Cultrex 3-D Spheroid Colorimetric Assay, 96-well Summary

Provides a powerful tool for modeling tumor growth response in vitro using colorimetric detection.

Why Use Cultrex 3-D Spheroid Colorimetric Assay, 96-well?

The Cultrex 3-D Spheroid Colorimetric Proliferation/Viability Assay utilizes a 3-D Culture Qualified 96 Well Spheroid Formation Plate alongside a specialized Spheroid Formation ECM to drive aggregation and/or spheroid formation of cells. Upon completion of spheroid formation, the spheroid may be treated with pharmacological agents to evaluate tumor viability after drug treatment. Tumor spheroid expansion is visualized microscopically and can be quantified through image analysis software for real-time and label free evaluation. At the conclusion of the assay, cell viability may be assessed by absorbance using MTT. The 3-D Spheroid Colorimetric Proliferation/Viability Assay offers an in vitro, standardized, three-dimensional, high content format for inducing multicellular tumor spheroid (MCTS) formation and quantitifying cell viability within the spheroids in response to pharmacological treatment.

Kit Contents

• Cultrex Spheroid Formation Matrix
• 3-D Culture Qualified 96 Well Spheroid Formation Plate with Strip Seals
• MTT Reagent
• Detergent Reagent

Note: The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging.

Specifications

Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Mouse

Limitations

For research use only. Not for diagnostic use.

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Citations for Cultrex 3-D Spheroid Colorimetric Assay, 96-well

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. MicroRNA-205-5p inhibits three-dimensional spheroid proliferation of ErbB2-overexpressing breast epithelial cells through direct targeting of CLCN3
    Authors: T Takeno, T Hasegawa, H Hasegawa, Y Ueno, R Hamataka, A Nakajima, J Okubo, K Sato, T Sakamaki
    PeerJ, 2019-10-08;7(0):e7799.  2019-10-08
  2. Resistance to MAPK inhibitors in melanoma involves activation of the IGF-1R-MEK5-Erk5 pathway
    Authors: L Benito-Jar, M Díaz-Martí, N Arellano-S, P Vaquero-Mo, A Esparís-Og, J Teixidó
    Cancer Res., 2019-03-04;0(0):.  2019-03-04
  3. An actionable axis linking NFATc2 to EZH2 controls the EMT-like program of melanoma cells
    Authors: V Perotti, P Baldassari, A Molla, G Nicolini, I Bersani, G Grazia, F Benigni, A Maurichi, M Santinami, A Anichini, R Mortarini
    Oncogene, 2019-02-01;0(0):.  2019-02-01
  4. Ablation of the ASCT2 (SLC1A5) gene encoding a neutral amino acid transporter reveals transporter plasticity and redundancy in cancer cells
    Authors: A Bröer, G Gauthier-C, F Rahimi, M van Gelder, D Dorsch, A Wegener, J Holst, S Bröer
    J. Biol. Chem., 2019-01-11;0(0):.  2019-01-11
  5. The Aged Microenvironment Influences the Tumorigenic Potential of Malignant Prostate Epithelial Cells
    Authors: D Bianchi-Fr, M Damodarasa, SA Hernandez, RM Gil da Cos, F Vakar-Lope, IM Coleman, MJ Reed, PS Nelson
    Mol. Cancer Res., 2018-09-17;17(1):321-331.  2018-09-17
  6. Extracellular signal regulated kinase 5 and inflammasome in progression of mesothelioma
    Authors: JK Thompson, A Shukla, AL Leggett, PB Munson, JM Miller, MB MacPherson, SL Beuschel, HI Pass, A Shukla
    Oncotarget, 2017-12-06;9(1):293-305.  2017-12-06

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Reviews for Cultrex 3-D Spheroid Colorimetric Assay, 96-well

Average Rating: 4 (Based on 2 Reviews)

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Cultrex 3D Spheroid Colorimetric Assay, 96 well
By Anonymous on 04/02/2018
Cultrex 3D Spheroid Colorimetric Assay, 96 well 3511-096-K
Cultrex 3D Spheroid Colorimetric Assay, 96 well
By Scott Schachtele on 12/06/2016

We use this to isolate stem cells and grow them in spheroid to test different experimental drugs for their efficacy to kill cancer stem cells .