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Cultrex Antibiotic-free Mouse Laminin I, Pathclear

Catalog #: 3401-010-02
1 Citation Datasheet / COA / SDS
Mouse Laminin I purified and qualified for use in antibiotic-free cell culture
Catalog # Availability Size / Price Qty
3401-010-02
Product Details
Citations (1)
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Cultrex Antibiotic-free Mouse Laminin I, Pathclear Summary

Cultrex Antibiotic-Free Mouse Laminin I is a purified basement membrane protein that has been developed and qualified as a extracellular matrix coating solution for cell attachment to tissue culture vessels.
 

Key Benefits

• Produced and qualified for use in cell culture
• Antibiotic-free formulation
• Pathclear-tested to ensure a pathogen and virus-free material
• Source tissue is LDEV-free

Why Use Cultrex Antibiotic-free Mouse Laminin I?

Cultrex Antibiotic-Free Mouse Laminin I is purified from Engelbreth-Holm-Swarm (EHS) tumor and is provided in antibiotic free medium. Laminin I is a major component of the basement membrane which is a continuous sheet of specialized extracellular matrix that forms an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that plays an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation.

Negative for the presence of bacteria and fungi.

Specifications

Source
Murine Engelbreth-Holm-Swarm (EHS) tumor
Sterility Testing
No bacterial or fungal growth detected following 14 days in culture
Testing Cell Culture
Supports cell attachment and spreading of MG63 human osteosarcoma cells.
Viral Testing
Tested negative by PCR test for a total of 31 organisms and viruses, including: mycoplasma, 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, and 13 additional murine infectious agents including LDEV.
Stability
Product is stable for a minimum of 3 months from date of shipment. See lot specific Certificate of Analysis for expiration date.
Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Species
Mouse

Limitations

For research use only. Not for diagnostic use.

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Citation for Cultrex Antibiotic-free Mouse Laminin I, Pathclear

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Live imaging of mRNA using RNA-stabilized fluorogenic proteins
    Authors: J Wu, S Zaccara, D Khuperkar, H Kim, ME Tanenbaum, SR Jaffrey
    Nat. Methods, 2019-08-30;16(9):862-865.  2019-08-30

FAQs

  1. What is the recommended working concentration for Cultrex Laminin I?

    • The recommended working concentration for thin coating is 0.05-10 µg/cm2 . However, conditions must be optimized for each cell line or model. Cultrex Laminin I should be used at 6 mg/mL for 3-D culture applications.

       

  2. Can Cultrex Mouse Laminin I be used on glass cover slips?

    • Yes. Coating glass cover slips with Cultrex Laminin I is quite common and has been published extensively.

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