What type of culture plates are recommended for organoid culture and other cultures using Cultrex BME products?

Name: Cultrex Basement Membrane Extract, Type 2, Pathclear
Brand: R&D Systems
SKU: 3532-010-02
Rating: 5 (1 reviews)

Any plate that is tissue culture treated will bind Cultrex domes/Cultrex coatings. Non-treated or low attachment plates are not recommended for organoid growth and for other cell cultures using coated BME. The domes will float away if non-treated or low-attachment plates are used. Bio-Techne has used the folllowing plates: Corning 96-well, Catalog # 3595; Corning 24-well, Catalog #3526; ThermoFisher 6-well, Catalog # 140675.

Cultrex Basement Membrane Extract, Type 2, Pathclear

Catalog #: 3532-010-02
22 Citations 1 Review Datasheet / COA / SDS

For Robust Organoid Cultures - Basement Membrane Extract
Try it on your cultures! Request a Sample of Cultrex BME, Type 2

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Cultrex Basement Membrane Extract, Type 2

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Citations (22)

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Reviews (1)

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Cultrex Basement Membrane Extract, Type 2, Pathclear Summary

Cultrex Basement Membrane Extract (BME), Type 2 is specifically designed to support the establishment and expansion of robust organoid cultures. It's composition mimics the in vivo microenvironment to improve take rate and growth of organoid progenitor cells.

Key Benefits

• Qualified for use in organoid cell culture
• Commonly used robust and established organoid systems
• Quality controlled for peformance consistency

Why Use Cultrex BME, Type 2?

Cultrex Basement Membrane Extract (BME), Type 2 is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. This extract provides a natural extracellular matrix hydrogel that polymerizes at 37°C to form a reconstituted basement membrane. Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. The major components of BME include laminin, collagen IV, entactin, and heparan sulfate proteoglycans.

Cultrex RGF BME, Type 2 provides a proprietary formulation that has a high storage modulus and is designed for use in robust tissue organoid culture as well as other applications requiring an extracellular matrix scaffold.

Specifications

Source

Murine Engelbreth-Holm-Swarm (EHS) tumor

Sterility Testing

No bacterial or fungal growth detected following 14 days in culture

Testing Cell Culture

Organoid Culture - Cultrex BME, Type 2 supports growth and expansion of mouse small intestine organoid progenitor cells.

Gelling Assay - Cultrex BME, Type 2 gels in less than 30 minutes at 37 °C, and maintains the gelled form in culture medium for a minimum of 7 days at 37 °C.

Dome Assay Cultrex BME, Type 2 forms and maintains stable 3-D dome structures on cell culture plates.

Tube Formation Assay - Cultrex BME, Type 2 supports formation of capillary-like structures by human (HBMVEC; HUVEC) or mouse (SVEC4-10) endothelial cells.

Viral Testing

Tested negative by PCR test for a total of 31 organisms and viruses, including: mycoplasma, 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, and 13 additional murine infectious agents including LDEV.

Stability

Product is stable for at least two years from date of manufacture when stored at ≤ -70 °C. See lot specific Certificate of Analysis for expiration date.

Shipping Conditions

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.

Storage

Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Species

Mouse

Limitations

For research use only. Not for diagnostic use.

Product Datasheets

Product Datasheet

COA

Citations for Cultrex Basement Membrane Extract, Type 2, Pathclear

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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Mechanisms that clear mutations drive field cancerization in mammary tissue
Authors: Ciwinska, M;Messal, HA;Hristova, HR;Lutz, C;Bornes, L;Chalkiadakis, T;Harkes, R;Langedijk, NSM;Hutten, SJ;Menezes, RX;Jonkers, J;Prekovic, S;Grand Challenge PRECISION consortium, ;Simons, BD;Scheele, CLGJ;van Rheenen, J;
Nature 2024-09-01
Enhanced Maturity and Functionality of Vascularized Human Liver Organoids through 3D Bioprinting and Pillar Plate Culture
Authors: Lekkala, VKR;Shrestha, S;Al Qaryoute, A;Dhinoja, S;Acharya, P;Raheem, A;Jagadeeswaran, P;Lee, MY;
bioRxiv : the preprint server for biology 2024-08-22
Neuro-mesenchymal interaction mediated by a ?2 adrenergic-nerve growth factor feedforward loop promotes colorectal cancer progression
Authors: Kobayashi, H;Iida, T;Ochiai, Y;Malagola, E;Zhi, X;White, RA;Qian, J;Wu, F;Waterbury, QT;Tu, R;Zheng, B;LaBella, JS;Zamechek, LB;Ogura, A;Woods, SL;Worthley, DL;Enomoto, A;Wang, TC;
Cancer discovery 2024-08-13
CRISPR/Cas9-edited ROS1?+?non-small cell lung cancer cell lines highlight differential drug sensitivity in 2D vs 3D cultures while reflecting established resistance profiles
Authors: Terrones, M;Deben, C;Rodrigues-Fortes, F;Schepers, A;de Beeck, KO;Van Camp, G;Vandeweyer, G;
Journal of translational medicine 2024-03-03
A Colonic Organoid Model Challenged with the Large Toxins of Clostridioides difficile TcdA and TcdB Exhibit Deregulated Tight Junction Proteins
Authors: Schneemann, M;Heils, L;Moos, V;Wei beta, F;Krug, SM;Weiner, J;Beule, D;Gerhard, R;Schulzke, JD;Bücker, R;
Toxins 2023-11-04
A Protocol for Organoids from the Urine of Bladder Cancer Patients
Authors: Walz, S;Pollehne, P;Geng, R;Schneider, J;Maas, M;Aicher, WK;Stenzl, A;Amend, B;Harland, N;
Cells 2023-08-31
TRPV1 controls innate immunity during Citrobacter rodentium enteric infection
Authors: Cremin, M;Tay, E;Ramirez, VT;Murray, K;Nichols, RK;Brust-Mascher, I;Reardon, C;
bioRxiv : the preprint server for biology 2023-07-27
ATG16L1 protects from interferon-gamma-induced cell death in the small intestinal crypt
Authors: EG Foerster, DKL Tsang, S Goyal, SJ Robertson, LM Robert, H Maughan, CJ Streutker, SE Girardin, DJ Philpott
Mucosal Immunology, 2023-02-14;0(0):. 2023-02-14
Modeling bile duct ischemia and reoxygenation injury inï¿½human cholangiocyte organoids for screening of novel cholangio-protective agents
Authors: S Shi, HP Roest, TPP van den Bo, MJC Bijvelds, MU Boehnert, J de Jonge, SO Dekker, AAF de Vries, HR de Jonge, MMA Verstegen, LJW van der La
EBioMedicine, 2023-01-04;88(0):104431. 2023-01-04
The molecular network of the proteasome machinery inhibition response is orchestrated by HSP70, revealing vulnerabilities in cancer cells
Authors: M Oro?, M Grochowski, A Jaiswar, J Legierska, K Jastrz?bsk, M Nowak-Niez, M Ko?os, W Ka?miercza, T Olesi?ski, M Lenarcik, M Cybulska, M Mikula, A ?ylicz, M Mi?czy?ska, K Zettl, JR Wi?niewski, D Walerych
Cell Reports, 2022-09-27;40(13):111428. 2022-09-27

Jejunum-derived NF-kappaB reporter organoids as 3D models for the study of TNF-alpha-induced inflammation
Authors: H Daghero, F Doffe, B Varela, V Yozzi, JM Verdes, M Crispo, M Bollati-Fo, R Pagotto
Scientific Reports, 2022-08-24;12(1):14425. 2022-08-24

The cGAS-STING pathway drives type I IFN immunopathology in COVID-19
Authors: J Di Domizio, MF Gulen, F Saidoune, VV Thacker, A Yatim, K Sharma, T Nass, E Guenova, M Schaller, C Conrad, C Goepfert, L De Leval, C von Garnie, S Berezowska, A Dubois, M Gilliet, A Ablasser
Nature, 2022-01-19;0(0):. 2022-01-19

Clinical stage drugs targeting inhibitor of apoptosis proteins purge episomal Hepatitis B viral genome in preclinical models
Authors: MP Clark, T Huynh, S Rao, L Mackiewicz, H Mason, S Romal, MD Stutz, SH Ahn, L Earnest, V Sozzi, M Littlejohn, BM Tran, N Wiedemann, E Vincan, J Torresi, HJ Netter, T Mahmoudi, P Revill, M Pellegrini, G Ebert
Cell Death & Disease, 2021-06-23;12(7):641. 2021-06-23

Primary Chicken and Duck Endothelial Cells Display a Differential Response to Infection with Highly Pathogenic Avian Influenza Virus
Authors: ZWM Tong, AC Karawita, C Kern, H Zhou, JE Sinclair, L Yan, KY Chew, S Lowther, L Trinidad, A Challagull, KA Schat, ML Baker, KR Short
Genes, 2021-06-10;12(6):. 2021-06-10

A genome-wide CRISPR screen identifies UFMylation and TRAMP-like complexes as host factors required for hepatitis A virus infection
Authors: J Kulsuptrak, R Wang, NL Meyers, M Ott, AS Puschnik
Cell Reports, 2021-03-16;34(11):108859. 2021-03-16

Functional Radiogenetic Profiling Implicates ERCC6L2 in Non-homologous End Joining
Authors: P Francica, M Mutlu, VA Blomen, C Oliveira, Z Nowicka, A Trenner, NM Gerhards, P Bouwman, E Stickel, ML Hekkelman, L Lingg, I Klebic, M van de Ven, R de Korte-G, D Howald, J Jonkers, AA Sartori, W Fendler, JR Chapman, T Brummelkam, S Rottenberg
Cell Rep, 2020-08-25;32(8):108068. 2020-08-25

High glucose levels increase influenza-associated damage to the pulmonary epithelial-endothelial barrier
Authors: KD Hulme, L Yan, RJ Marshall, CJ Bloxham, KR Upton, SZ Hasnain, H Bielefeldt, Z Loh, K Ronacher, KY Chew, LA Gallo, KR Short
Elife, 2020-07-22;9(0):. 2020-07-22

LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer
Authors: X Jin, LP Ge, DQ Li, ZM Shao, GH Di, XE Xu, YZ Jiang
Mol. Cancer, 2020-05-11;19(1):87. 2020-05-11

Breast cancer organoids from a patient with giant papillary carcinoma as a high-fidelity model
Authors: X Li, B Pan, X Song, N Li, D Zhao, M Li, Z Zhao
Cancer Cell Int., 2020-03-18;20(0):86. 2020-03-18

Radiosensitivity Is an Acquired Vulnerability of PARPi-Resistant BRCA1-Deficient Tumors
Authors: M Barazas, A Gasparini, Y Huang, A Küçükosman, S Annunziato, P Bouwman, W Sol, A Kersbergen, N Proost, R de Korte-G, M van de Ven, J Jonkers, GR Borst, S Rottenberg
Cancer Res., 2018-12-10;79(3):452-460. 2018-12-10

Basement Membrane-Based Glucose Sensor Coatings Enhance Continuous Glucose Monitoring in Vivo.
Authors: Klueh U, Qiao Y, Czajkowski C, Ludzinska I, Antar O, Kreutzer D
J Diabetes Sci Technol, 2015-08-25;9(5):957-65. 2015-08-25

In vitro differentiation of human amniotic epithelial cells into insulin-producing 3D spheroids.
Authors: Okere B, Alviano F, Costa R, Quaglino D, Ricci F, Dominici M, Paolucci P, Bonsi L, Iughetti L
Int J Immunopathol Pharmacol, 2015-07-27;28(3):390-402. 2015-07-27

FAQs

What kinds of tumor cells or biopsy specimens grow in vivo with Cultrex® BME?
- Many cell lines and tumor biopsy specimens (usually cut into small fragments) have been found to grow in vivo when implanted with Cultrex^® BME. These include melanoma, intestinal, prostate, breast, lung, renal, and liver cancers as well as the 3T3 mouse embryonic fibroblast cell line.
How does Cultrex^® Basement Membrane Extract (BME) promote cell differentiation?
- All epithelial and endothelial cells are in contact with a basement membrane matrix on at least one of their surfaces. By providing them with their natural matrix in vitro as a substrate for the cells that provides biological cues, the cells can assume a more physiological morphology (i.e. correct shape) and begin expression of cell-lineage specific proteins. Two-dimensional plastic surfaces, in combination with serum-containing media, cause cells to flatten, proliferate and de-differentiate.
How should Cultrex Basement Membrane Extract (BME) be stored and handled?
- For best practices for handling our BME products, please refer to our helpful guide at the following link: https://www.bio-techne.com/reagents/cell-culture-reagents/cultrex-bme-and-ecm-proteins/cell-culture-matrix-for-your-research
What type of culture plates are recommended for organoid culture and other cultures using Cultrex BME products?
- Any plate that is tissue culture treated will bind Cultrex domes/Cultrex coatings. Non-treated or low attachment plates are not recommended for organoid growth and for other cell cultures using coated BME. The domes will float away if non-treated or low-attachment plates are used. Bio-Techne has used the folllowing plates: Corning 96-well, Catalog # 3595; Corning 24-well, Catalog #3526; ThermoFisher 6-well, Catalog # 140675.