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Cultrex Rat Collagen I

Catalog #: 3440-100-01
55 Citations Datasheet / COA / SDS
Purified stromal extracellular matrix protein that promotes cell adhesion for cell and tissue cultures
Catalog # Availability Size / Price Qty
3440-005-01
3440-100-01
Product Details
Citations (55)
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Summary Product Datasheets

Cultrex Rat Collagen I Summary

Cultrex Rat Collagen I is a purified stromal extracellular matrix protein that promotes cell adhesion for cell and tissue culture. It may also be used to supplement customized coatings, hydrogels, bioinks, or medium formulations for cell culture.
 

Key Benefits

• Qualified for general cell culture applications
• May be used as a hydrogel or thin coating for tissue culture vessels
• Material provided at a standard concentration of ~5 mg/mL

Why Use Cultrex Rat Collagen I?

Cultrex Rat Collagen I is purified from rat tail tendons and is provided at a high concentration that is capable of polymerizing to form a hydrogel. Collagen I is the major structural component of extracellular matrices (ECM) found in connective tissue and internal organs, but is most prevalent in the dermis, tendons, and bone. It is a 300 kDa molecule composed of two alpha1(I) chains and one alpha2(I) chain that spontaneously forms a triple helix scaffold at a neutral pH and 37̊ C. This phenomenon can be exploited to promote cell attachment, growth, differentiation, migration, and tissue morphogenesis during development. To provide the most standardized Collagen I, a special process is employed to provide material at a standard concentration of approximately 5 mg/mL.

Specifications

Source
Rat tail tendons
Sterility Testing
No bacterial or fungal growth detected following 14 days in culture
Testing Cell Culture
Supports cell attachment and spreading of HT1080 human fibrosarcoma cells.
Viral Testing
No mycoplasma contamination detected by PCR.
Stability
Product is stable for a minimum of 2 months if stored at 2-8 °C. See lot specific Certificate of Analysis for expiration date.
Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at 2 - 8 °C.
Species
Rat

Limitations

For research use only. Not for diagnostic use.

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Citations for Cultrex Rat Collagen I

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

55 Citations: Showing 1 - 10
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  1. Generation of human iPSC-derived 3D bile duct within liver organoid by incorporating human iPSC-derived blood vessel
    Authors: Carolina, E;Kuse, Y;Okumura, A;Aoshima, K;Tadokoro, T;Matsumoto, S;Kanai, E;Okumura, T;Kasai, T;Yamabe, S;Nishikawa, Y;Yamaguchi, K;Furukawa, Y;Tanimizu, N;Taniguchi, H;
    Nature communications  2024-08-28
  2. Human organoids with an autologous tissue-resident immune compartment
    Authors: Recaldin, T;Steinacher, L;Gjeta, B;Harter, MF;Adam, L;Kromer, K;Mendes, MP;Bellavista, M;Nikolaev, M;Lazzaroni, G;Krese, R;Kilik, U;Popovic, D;Stoll, B;Gerard, R;Bscheider, M;Bickle, M;Cabon, L;Camp, JG;Gjorevski, N;
    Nature  2024-08-14
  3. GRT-X Stimulates Dorsal Root Ganglia Axonal Growth in Culture via TSPO and Kv7.2/3 Potassium Channel Activation
    Authors: El Chemali, L;Boutary, S;Liu, S;Liu, GJ;Middleton, RJ;Banati, RB;Bahrenberg, G;Rupprecht, R;Schumacher, M;Massaad-Massade, L;
    International journal of molecular sciences  2024-07-03
  4. Multicompartmentalized microvascularized tumor-on-a-chip to study tumor-stroma interactions and drug resistance in ovarian cancer
    Authors: Plesselova, S;Calar, K;Axemaker, H;Sahly, E;de la Puente, P;
    bioRxiv : the preprint server for biology  2024-06-02
  5. Differential Smad2/3 linker phosphorylation is a crosstalk mechanism of Rho/ROCK and canonical TGF-?3 signaling in tenogenic differentiation
    Authors: Melzer, M;Niebert, S;Heimann, M;Ullm, F;Pompe, T;Scheiner-Bobis, G;Burk, J;
    Scientific reports  2024-05-06
  6. KCNJ2 inhibition mitigates mechanical injury in a human brain organoid model of traumatic brain injury
    Authors: Lai, JD;Berlind, JE;Fricklas, G;Lie, C;Urenda, JP;Lam, K;Sta Maria, N;Jacobs, R;Yu, V;Zhao, Z;Ichida, JK;
    Cell stem cell  2024-04-04
  7. Inactivation of kindlin-3 increases human melanoma aggressiveness through the collagen-activated tyrosine kinase receptor DDR1
    Authors: Reger De Moura, C;Louveau, B;Jouenne, F;Vilquin, P;Battistella, M;Bellahsen-Harrar, Y;Sadoux, A;Menashi, S;Dumaz, N;Lebbé, C;Mourah, S;
    Oncogene  2024-04-03
  8. Development of a 3D Perfused In Vitro System to Assess Proangiogenic Properties of Compounds
    Authors: Alm, J;Fischer, B;Burger, AE;Moretti, F;
    Methods and protocols  2023-12-09
  9. Dystrophin deficiency impairs cell junction formation during embryonic myogenesis
    Authors: Mozin, E;Massouridès, E;Mournetas, V;Lièvre, C;Bourdon, A;Jackson, DL;Packer, JS;Trapnell, C;Guiner, CL;Adjali, O;Pinset, C;Mack, DL;Dupont, JB;
    bioRxiv : the preprint server for biology  2023-12-07
  10. Polyethylene Glycol and Caspase Inhibitor Emricasan Alleviates Cold Injury in Primary Rat Hepatocytes
    Authors: Chen, H;Ellis, BW;Dinicu, AT;Mojoudi, M;Wilks, BT;Tessier, SN;Toner, M;Uygun, K;Uygun, BE;
    Research square  2023-11-28
  11. Impairment of exocytotic transmitter release by decynium-22 through an inhibition of ion channels
    Authors: Haar, G;Hrachova, K;Wagner, T;Boehm, S;Schicker, K;
    Frontiers in pharmacology  2023-10-10
  12. A model of hepatic steatosis with declined viability and function in a liver-organ-on-a-chip
    Authors: Wiriyakulsit, N;Keawsomnuk, P;Thongin, S;Ketsawatsomkron, P;Muta, K;
    Scientific reports  2023-10-09
  13. Conserved cardiolipin-mitochondrial ADP/ATP carrier interactions assume distinct structural and functional roles that are clinically relevant
    Authors: Senoo, N;Chinthapalli, DK;Baile, MG;Golla, VK;Saha, B;Ogunbona, OB;Saba, JA;Munteanu, T;Valdez, Y;Whited, K;Chorev, D;Alder, NN;May, ER;Robinson, CV;Claypool, SM;
    bioRxiv : the preprint server for biology  2023-05-06
  14. High-throughput 3D microvessel-on-a-chip model to study defective angiogenesis in systemic sclerosis
    Authors: B Kramer, C Corallo, A van den He, J Crawford, T Olivier, E Elstak, N Giordano, P Vulto, HL Lanz, RAJ Janssen, MA Tessari
    Scientific Reports, 2022-10-08;12(1):16930.  2022-10-08
  15. Blocking CHOP-dependent TXNIP shuttling to mitochondria attenuates albuminuria and mitigates kidney injury in nephrotic syndrome
    Authors: SJ Park, Y Kim, C Li, J Suh, J Sivapackia, TM Goncalves, G Jarad, G Zhao, F Urano, V Sharma, YM Chen
    Proceedings of the National Academy of Sciences of the United States of America, 2022-08-22;119(35):e2116505119.  2022-08-22
  16. TWIST1 methylation by SETD6 selectively antagonizes LINC-PINT expression in glioma
    Authors: L Admoni-Eli, T Elbaz, A Chopra, G Shapira, MT Bedford, CJ Fry, N Shomron, K Biggar, M Feldman, D Levy
    Nucleic Acids Research, 2022-07-08;0(0):.  2022-07-08
  17. Chronic Low Dose Morphine Does Not Alter Two In Vitro BBB Models
    Authors: J Marino, ME Maubert, JM Lawrence, B Wigdahl, MR Nonnemache
    Brain sciences, 2022-07-06;12(7):.  2022-07-06
  18. Plasminogen Binding and Activation at the Mesothelial Cell Surface Promotes Invasion through a Collagen Matrix
    Authors: Z Ditzig, CM Wilson, J Salas, KM Serve
    Oncogene, 2022-05-26;23(11):.  2022-05-26
  19. Dynamic early clusters of nodal proteins contribute to node of Ranvier assembly during myelination of peripheral neurons
    Authors: EL Malavasi, A Ghosh, DG Booth, M Zagnoni, DL Sherman, PJ Brophy
    Elife, 2021-07-09;10(0):.  2021-07-09
  20. Transferrin-functionalized lipid nanoparticles for curcumin brain delivery
    Authors: AR Neves, PL van der, JF Queiroz, M Pinheiro, S Reis
    Journal of biotechnology, 2021-03-13;331(0):108-117.  2021-03-13
  21. Pragmatic recruitment of memantine as the capping group for the design of HDAC inhibitors: A preliminary attempt to unravel the enigma of glioblastoma
    Authors: K Nepali, TI Hsu, CM Hsieh, WL Lo, MJ Lai, KC Hsu, TE Lin, JY Chuang, JP Liou
    European Journal of Medicinal Chemistry, 2021-03-12;217(0):113338.  2021-03-12
  22. The in vitro multilineage differentiation and maturation of lung and airway cells from human pluripotent stem cell-derived lung progenitors in 3D
    Authors: AL Rodrigues, HY Liu, YW Chen, M Porotto, A Moscona, HW Snoeck
    Nature Protocols, 2021-03-01;0(0):.  2021-03-01
  23. Establishment of MHHi001-A-5, a GCaMP6f and RedStar dual reporter human iPSC line for in vitro and in vivo characterization and in situ tracing of iPSC derivatives
    Authors: A Haase, T Kohrn, V Fricke, ME Ricci Sign, M Witte, G Göhring, I Gruh, U Martin
    Stem Cell Research, 2021-01-30;52(0):102206.  2021-01-30
  24. The ER Unfolded Protein Response Effector, ATF6, Reduces Cardiac Fibrosis and Decreases Activation of Cardiac Fibroblasts
    Authors: WT Stauffer, EA Blackwood, K Azizi, RJ Kaufman, CC Glembotski
    Int J Mol Sci, 2020-02-18;21(4):.  2020-02-18
  25. Biosynthetic CircRNA_001160 induced by PTBP1 regulates the permeability of BTB via the CircRNA_001160/miR-195-5p/ETV1 axis
    Authors: H Li, S Shen, X Ruan, X Liu, J Zheng, Y Liu, C Yang, D Wang, L Liu, J Ma, T Ma, P Wang, H Cai, Z Li, L Zhao, Y Xue
    Cell Death Dis, 2019-12-20;10(12):960.  2019-12-20
  26. Peptide hormone ELABELA enhances extravillous trophoblast differentiation, but placenta is not the major source of circulating ELABELA in pregnancy
    Authors: D Georgiadou, S Boussata, WHM Ranzijn, LEA Root, S Hillenius, JM Bij de Weg, CNH Abheiden, MA de Boer, JIP de Vries, TGM Vrijkotte, CB Lambalk, EAM Kuijper, GB Afink, M van Dijk
    Sci Rep, 2019-12-13;9(1):19077.  2019-12-13
  27. Development of a Gut-On-A-Chip Model for High Throughput Disease Modeling and Drug Discovery
    Authors: C Beaurivage, E Naumovska, YX Chang, ED Elstak, A Nicolas, H Wouters, G van Moolen, HL Lanz, SJ Trietsch, J Joore, P Vulto, RAJ Janssen, KS Erdmann, J Stallen, D Kurek
    Int J Mol Sci, 2019-11-12;20(22):.  2019-11-12
  28. Circular RNA USP1 regulates the permeability of blood-tumour barrier via miR-194-5p/FLI1 axis
    Authors: Y Gao, P Wu, Y Ma, Y Xue, Y Liu, J Zheng, X Liu, Q He, J Ma, L Liu, P Wang
    J. Cell. Mol. Med., 2019-10-26;0(0):.  2019-10-26
  29. Zika virus enhances monocyte adhesion and transmigration favoring viral dissemination to neural cells
    Authors: NV Ayala-Nune, G Follain, F Delalande, A Hirschler, E Partiot, GL Hale, BC Bollweg, J Roels, M Chazal, F Bakoa, M Carocci, S Bourdoulou, O Faklaris, SR Zaki, A Eckly, B Uring-Lamb, F Doussau, S Cianferani, C Carapito, FMJ Jacobs, N Jouvenet, JG Goetz, R Gaudin
    Nat Commun, 2019-09-27;10(1):4430.  2019-09-27
  30. Discovery of endoplasmic reticulum calcium stabilizers to rescue ER-stressed podocytes in nephrotic syndrome
    Authors: SJ Park, Y Kim, SM Yang, MJ Henderson, W Yang, M Lindahl, F Urano, YM Chen
    Proc. Natl. Acad. Sci. U.S.A., 2019-06-24;0(0):.  2019-06-24
  31. Tumour-treating fields (TTFields): Investigations on the mechanism of action by electromagnetic exposure of cells in telophase/cytokinesis
    Authors: L Berkelmann, A Bader, S Meshksar, A Dierks, G Hatipoglu, JK Krauss, K Schwabe, D Manteuffel, A Ngezahayo
    Sci Rep, 2019-05-14;9(1):7362.  2019-05-14
  32. Formation of three-dimensional tubular endothelial cell networks under defined serum-free cell culture conditions in human collagen hydrogels
    Authors: B Andrée, H Ichanti, S Kalies, A Heisterkam, S Strau beta, PM Vogt, A Haverich, A Hilfiker
    Sci Rep, 2019-04-01;9(1):5437.  2019-04-01
  33. Randomly Distributed K14+ Breast Tumor Cells Polarize to the Leading Edge and Guide Collective Migration in Response to Chemical and Mechanical Environmental Cues
    Authors: PY Hwang, A Brenot, AC King, GD Longmore, SC George
    Cancer Res., 2019-03-12;79(8):1899-1912.  2019-03-12
  34. Interaction of Treponema pallidum, the syphilis spirochete, with human platelets
    Authors: B Church, E Wall, JR Webb, CE Cameron
    PLoS ONE, 2019-01-18;14(1):e0210902.  2019-01-18
  35. Mechanism of piR-DQ590027/MIR17HG regulating the permeability of glioma conditioned normal BBB
    Authors: X Leng, J Ma, Y Liu, S Shen, H Yu, J Zheng, X Liu, L Liu, J Chen, L Zhao, X Ruan, Y Xue
    J. Exp. Clin. Cancer Res., 2018-10-11;37(1):246.  2018-10-11
  36. Increased macrophages and�changed brain endothelial cell gene expression in the frontal cortex of people with schizophrenia displaying inflammation
    Authors: HQ Cai, VS Catts, MJ Webster, C Galletly, D Liu, M O'Donnell, TW Weickert, CS Weickert
    Mol. Psychiatry, 2018-09-13;0(0):.  2018-09-13
  37. Smooth muscle cells differentiated from human mesenchymal stem cells regulated by microRNA (miR)-503 and miR-222-5p are suitable for vascular tissue engineering
    Authors: W Gu, X Hong, A Le Bras, WN Nowak, S Issa Bhalo, J Deng, Y Xie, Y Hu, XZ Ruan, Q Xu
    J. Biol. Chem., 2018-04-11;0(0):.  2018-04-11
  38. Protein S and Gas6 induce efferocytosis of HIV-1-infected cells
    Authors: BA Chua, JA Ngo, K Situ, CM Ramirez, H Nakano, K Morizono
    Virology, 2018-01-02;515(0):176-190.  2018-01-02
  39. Metabolite Profile and Antiproliferative Effects in HaCaT Cells of a Salix reticulata Extract.
    Authors: Corradi E, Schmidt N, Raber N, De Mieri M, Hamburger M, Butterweck V, Potterat O
    Planta Med, 2017-04-27;83(14):1149-1158.  2017-04-27
  40. Enhanced transport of plant-produced rabies single chain antibody-RVG peptide fusion protein across an in cellulo blood-brain barrier device
    Authors: W Phoolcharo, C Prehaud, CJ van Dollew, L Both, A da Costa, M Lafon, JK Ma
    Plant Biotechnol. J, 2017-04-19;0(0):.  2017-04-19
  41. Knockdown of long non-coding RNA XIST increases blood-tumor barrier permeability and inhibits glioma angiogenesis by targeting miR-137
    Authors: H Yu, Y Xue, P Wang, X Liu, J Ma, J Zheng, Z Li, Z Li, H Cai, Y Liu
    Oncogenesis, 2017-03-13;6(3):e303.  2017-03-13
  42. PVT1 affects growth of glioma microvascular endothelial cells by negatively regulating miR-186
    Authors: Y Ma, P Wang, Y Xue, C Qu, J Zheng, X Liu, J Ma, Y Liu
    Tumour Biol., 2017-03-01;39(3):1010428317694.  2017-03-01
  43. Multifunctional LUV liposomes decorated for BBB and amyloid targeting. A. In vitro proof-of-concept
    Authors: K Papadia, E Markoutsa, S Mourtas, AD Giannou, B La Ferla, F Nicotra, M Salmona, P Klepetsani, GT Stathopoul, SG Antimisiar
    Eur J Pharm Sci, 2017-02-11;101(0):140-148.  2017-02-11
  44. Trojan Horse Transit Contributes to Blood-Brain Barrier Crossing of a Eukaryotic Pathogen
    Authors: FH Santiago-T, MD Onken, JA Cooper, RS Klein, TL Doering
    MBio, 2017-01-31;8(1):.  2017-01-31
  45. Mesencephalic Astrocyte-Derived Neurotrophic Factor as a Urine Biomarker for Endoplasmic Reticulum Stress-Related Kidney Diseases.
    Authors: Kim Y, Lee H, Manson S, Lindahl M, Evans B, Miner J, Urano F, Chen Y
    J Am Soc Nephrol, 2016-03-03;27(10):2974-2982.  2016-03-03
  46. Maspin Expression in Prostate Tumor Cells Averts Stemness and Stratifies Drug Sensitivity.
    Authors: Bernardo M, Kaplun A, Dzinic S, Li X, Irish J, Mujagic A, Jakupovic B, Back J, Van Buren E, Han X, Dean I, Chen Y, Heath E, Sakr W, Sheng S
    Cancer Res, 2015-07-24;75(18):3970-9.  2015-07-24
  47. DOCK8 regulates lymphocyte shape integrity for skin antiviral immunity.
    Authors: Zhang, Qian, Dove, Christop, Hor, Jyh Lian, Murdock, Heardley, Strauss-Albee, Dara M, Garcia, Jordan A, Mandl, Judith N, Grodick, Rachael, Jing, Huie, Chandler-Brown, Devon B, Lenardo, Timothy, Crawford, Greg, Matthews, Helen F, Freeman, Alexandr, Cornall, Richard, Germain, Ronald N, Mueller, Scott N, Su, Helen C
    J Exp Med, 2014-11-24;211(13):2549-66.  2014-11-24
  48. MiR-18a increased the permeability of BTB via RUNX1 mediated down-regulation of ZO-1, occludin and claudin-5.
    Authors: Miao Y, Zhao Y, Zhao L, Wang P, Liu Y, Ma J, Xue Y
    Cell Signal, 2014-10-29;27(1):156-67.  2014-10-29
  49. Biphasic modulation of paracellular claudin-5 expression in mouse brain endothelial cells is mediated through the phosphoinositide-3-kinase/AKT pathway.
    Authors: Camire R, Beaulac H, Brule S, McGregor A, Lauria E, Willis C
    J Pharmacol Exp Ther, 2014-10-03;351(3):654-62.  2014-10-03
  50. RPTPalpha controls epithelial adherens junctions, linking E-cadherin engagement to c-Src-mediated phosphorylation of cortactin.
    Authors: Truffi M, Dubreuil V, Liang X, Vacaresse N, Nigon F, Han S, Yap A, Gomez G, Sap J
    J Cell Sci, 2014-03-20;127(0):2420-32.  2014-03-20
  51. Glycosylation at Asn211 regulates the activation state of the discoidin domain receptor 1 (DDR1).
    Authors: Fu H, Valiathan R, Payne L, Kumarasiri M, Mahasenan K, Mobashery S, Huang P, Fridman R
    J Biol Chem, 2014-02-07;289(13):9275-87.  2014-02-07
  52. Jab1 regulates Schwann cell proliferation and axonal sorting through p27.
    Authors: Porrello, Emanuela, Rivellini, Cristina, Dina, Giorgia, Triolo, Daniela, Del Carro, Ubaldo, Ungaro, Daniela, Panattoni, Martina, Feltri, Maria La, Wrabetz, Lawrence, Pardi, Ruggero, Quattrini, Angelo, Previtali, Stefano
    J Exp Med, 2013-12-16;211(1):29-43.  2013-12-16
  53. Transcellular targeting of fiber- and hexon-modified adenovirus vectors across the brain microvascular endothelial cells in vitro.
    Authors: Laakkonen, Johanna, Engler, Tatjana, Romero, Ignacio, Weksler, Babette, Couraud, Pierre-O, Kreppel, Florian, Kochanek, Stefan
    PLoS ONE, 2012-09-27;7(9):e45977.  2012-09-27
  54. Targeted protein engineering provides insights into binding mechanism and affinities of bacterial collagen adhesins.
    Authors: Ross C, Liang X, Liu Q, Murray B, Hook M, Ganesh V
    J Biol Chem, 2012-08-03;287(41):34856-65.  2012-08-03
  55. Characterization of the interface between normal and transformed epithelial cells.
    Authors: Hogan C, Dupre-Crochet S, Norman M, Kajita M, Zimmermann C, Pelling AE, Piddini E, Baena-Lopez LA, Vincent JP, Itoh Y, Hosoya H, Pichaud F, Fujita Y
    Nat. Cell Biol., 2009-03-15;11(4):460-7.  2009-03-15

FAQs

  1. What is the difference between the standard Cultrex Rat Collagen I and the Cultrex 3-D Rat Collagen I?

    • Cultrex 3-D Rat Collagen I undergoes the same basic purification and efficacy tests as the standard collagen. However, it undergoes additional 3-D culture validation; it has been tested extensively for the ability to promote growth and differentiation of cell types, visualized by morphology in three dimensions in vitro.

  2. What are the differences between Cultrex Rat Collagen I and Cultrex Rat Collagen I, Low Viscosity?

    • Cultrex Rat Collagen I is provided at 5 mg/mL and is not pepsin treated, so it has intact telopeptides and is highly viscous. Cultrex Rat Collagen I, Low Viscosity is provided at 3 mg/mL, and it is easier to pipet. Both are provided in acetic acid and require pH neutralization prior to use. Please consult the provided product data sheets for pH neutralization instructions.

  3. Is Cultrex Rat Collagen I atelocollagen or telocollagen?

    • Cultrex Rat Collagen I is telocollagen.  

  4. After coating the Cultrex Rat Collagen I (catalog # 3440-100-01), there is a white film on the plate. What is this white film? Is this normal?

    • This white film is normal. The collagen coating can result in a slight white haziness to the plate, which could also be the result of excess salt if the plates are not well aspirated.

  5. Can the Organoid Harvesting Solution, Catalog # 3700-100-01,  be used to dissociate cells grown on a substrate of Cultrex Rat Collagen 1?

    • The Organoid Harvesting Solution works well with BME (Basement Membrane Extract).  For cells grown on Cultrex Rat Collagen 1, the recommended enzyme to use to dissociate cells is Collagenase. Collagenase treatment at 37oC for 30 minutes is recommended.

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