Cultrex Rat Collagen I
Cultrex Rat Collagen I Summary
Cultrex Rat Collagen I is a purified stromal extracellular matrix protein that promotes cell adhesion for cell and tissue culture. It may also be used to supplement customized coatings, hydrogels, bioinks, or medium formulations for cell culture.Key Benefits
• Qualified for general cell culture applications
• May be used as a hydrogel or thin coating for tissue culture vessels
• Material provided at a standard concentration of ~5 mg/mL
Why Use Cultrex Rat Collagen I?
Cultrex Rat Collagen I is purified from rat tail tendons and is provided at a high concentration that is capable of polymerizing to form a hydrogel. Collagen I is the major structural component of extracellular matrices (ECM) found in connective tissue and internal organs, but is most prevalent in the dermis, tendons, and bone. It is a 300 kDa molecule composed of two alpha1(I) chains and one alpha2(I) chain that spontaneously forms a triple helix scaffold at a neutral pH and 37̊ C. This phenomenon can be exploited to promote cell attachment, growth, differentiation, migration, and tissue morphogenesis during development. To provide the most standardized Collagen I, a special process is employed to provide material at a standard concentration of approximately 5 mg/mL.
Specifications
Limitations
For research use only. Not for diagnostic use.
Product Datasheets
Citations for Cultrex Rat Collagen I
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Generation of human iPSC-derived 3D bile duct within liver organoid by incorporating human iPSC-derived blood vessel
Authors: Carolina, E;Kuse, Y;Okumura, A;Aoshima, K;Tadokoro, T;Matsumoto, S;Kanai, E;Okumura, T;Kasai, T;Yamabe, S;Nishikawa, Y;Yamaguchi, K;Furukawa, Y;Tanimizu, N;Taniguchi, H;
Nature communications 2024-08-28
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Human organoids with an autologous tissue-resident immune compartment
Authors: Recaldin, T;Steinacher, L;Gjeta, B;Harter, MF;Adam, L;Kromer, K;Mendes, MP;Bellavista, M;Nikolaev, M;Lazzaroni, G;Krese, R;Kilik, U;Popovic, D;Stoll, B;Gerard, R;Bscheider, M;Bickle, M;Cabon, L;Camp, JG;Gjorevski, N;
Nature 2024-08-14
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GRT-X Stimulates Dorsal Root Ganglia Axonal Growth in Culture via TSPO and Kv7.2/3 Potassium Channel Activation
Authors: El Chemali, L;Boutary, S;Liu, S;Liu, GJ;Middleton, RJ;Banati, RB;Bahrenberg, G;Rupprecht, R;Schumacher, M;Massaad-Massade, L;
International journal of molecular sciences 2024-07-03
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Multicompartmentalized microvascularized tumor-on-a-chip to study tumor-stroma interactions and drug resistance in ovarian cancer
Authors: Plesselova, S;Calar, K;Axemaker, H;Sahly, E;de la Puente, P;
bioRxiv : the preprint server for biology 2024-06-02
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Differential Smad2/3 linker phosphorylation is a crosstalk mechanism of Rho/ROCK and canonical TGF-?3 signaling in tenogenic differentiation
Authors: Melzer, M;Niebert, S;Heimann, M;Ullm, F;Pompe, T;Scheiner-Bobis, G;Burk, J;
Scientific reports 2024-05-06
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KCNJ2 inhibition mitigates mechanical injury in a human brain organoid model of traumatic brain injury
Authors: Lai, JD;Berlind, JE;Fricklas, G;Lie, C;Urenda, JP;Lam, K;Sta Maria, N;Jacobs, R;Yu, V;Zhao, Z;Ichida, JK;
Cell stem cell 2024-04-04
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Inactivation of kindlin-3 increases human melanoma aggressiveness through the collagen-activated tyrosine kinase receptor DDR1
Authors: Reger De Moura, C;Louveau, B;Jouenne, F;Vilquin, P;Battistella, M;Bellahsen-Harrar, Y;Sadoux, A;Menashi, S;Dumaz, N;Lebbé, C;Mourah, S;
Oncogene 2024-04-03
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Development of a 3D Perfused In Vitro System to Assess Proangiogenic Properties of Compounds
Authors: Alm, J;Fischer, B;Burger, AE;Moretti, F;
Methods and protocols 2023-12-09
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Dystrophin deficiency impairs cell junction formation during embryonic myogenesis
Authors: Mozin, E;Massouridès, E;Mournetas, V;Lièvre, C;Bourdon, A;Jackson, DL;Packer, JS;Trapnell, C;Guiner, CL;Adjali, O;Pinset, C;Mack, DL;Dupont, JB;
bioRxiv : the preprint server for biology 2023-12-07
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Polyethylene Glycol and Caspase Inhibitor Emricasan Alleviates Cold Injury in Primary Rat Hepatocytes
Authors: Chen, H;Ellis, BW;Dinicu, AT;Mojoudi, M;Wilks, BT;Tessier, SN;Toner, M;Uygun, K;Uygun, BE;
Research square 2023-11-28
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Impairment of exocytotic transmitter release by decynium-22 through an inhibition of ion channels
Authors: Haar, G;Hrachova, K;Wagner, T;Boehm, S;Schicker, K;
Frontiers in pharmacology 2023-10-10
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A model of hepatic steatosis with declined viability and function in a liver-organ-on-a-chip
Authors: Wiriyakulsit, N;Keawsomnuk, P;Thongin, S;Ketsawatsomkron, P;Muta, K;
Scientific reports 2023-10-09
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Conserved cardiolipin-mitochondrial ADP/ATP carrier interactions assume distinct structural and functional roles that are clinically relevant
Authors: Senoo, N;Chinthapalli, DK;Baile, MG;Golla, VK;Saha, B;Ogunbona, OB;Saba, JA;Munteanu, T;Valdez, Y;Whited, K;Chorev, D;Alder, NN;May, ER;Robinson, CV;Claypool, SM;
bioRxiv : the preprint server for biology 2023-05-06
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High-throughput 3D microvessel-on-a-chip model to study defective angiogenesis in systemic sclerosis
Authors: B Kramer, C Corallo, A van den He, J Crawford, T Olivier, E Elstak, N Giordano, P Vulto, HL Lanz, RAJ Janssen, MA Tessari
Scientific Reports, 2022-10-08;12(1):16930. 2022-10-08
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Blocking CHOP-dependent TXNIP shuttling to mitochondria attenuates albuminuria and mitigates kidney injury in nephrotic syndrome
Authors: SJ Park, Y Kim, C Li, J Suh, J Sivapackia, TM Goncalves, G Jarad, G Zhao, F Urano, V Sharma, YM Chen
Proceedings of the National Academy of Sciences of the United States of America, 2022-08-22;119(35):e2116505119. 2022-08-22
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TWIST1 methylation by SETD6 selectively antagonizes LINC-PINT expression in glioma
Authors: L Admoni-Eli, T Elbaz, A Chopra, G Shapira, MT Bedford, CJ Fry, N Shomron, K Biggar, M Feldman, D Levy
Nucleic Acids Research, 2022-07-08;0(0):. 2022-07-08
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Chronic Low Dose Morphine Does Not Alter Two In Vitro BBB Models
Authors: J Marino, ME Maubert, JM Lawrence, B Wigdahl, MR Nonnemache
Brain sciences, 2022-07-06;12(7):. 2022-07-06
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Plasminogen Binding and Activation at the Mesothelial Cell Surface Promotes Invasion through a Collagen Matrix
Authors: Z Ditzig, CM Wilson, J Salas, KM Serve
Oncogene, 2022-05-26;23(11):. 2022-05-26
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Dynamic early clusters of nodal proteins contribute to node of Ranvier assembly during myelination of peripheral neurons
Authors: EL Malavasi, A Ghosh, DG Booth, M Zagnoni, DL Sherman, PJ Brophy
Elife, 2021-07-09;10(0):. 2021-07-09
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Transferrin-functionalized lipid nanoparticles for curcumin brain delivery
Authors: AR Neves, PL van der, JF Queiroz, M Pinheiro, S Reis
Journal of biotechnology, 2021-03-13;331(0):108-117. 2021-03-13
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Pragmatic recruitment of memantine as the capping group for the design of HDAC inhibitors: A preliminary attempt to unravel the enigma of glioblastoma
Authors: K Nepali, TI Hsu, CM Hsieh, WL Lo, MJ Lai, KC Hsu, TE Lin, JY Chuang, JP Liou
European Journal of Medicinal Chemistry, 2021-03-12;217(0):113338. 2021-03-12
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The in vitro multilineage differentiation and maturation of lung and airway cells from human pluripotent stem cell-derived lung progenitors in 3D
Authors: AL Rodrigues, HY Liu, YW Chen, M Porotto, A Moscona, HW Snoeck
Nature Protocols, 2021-03-01;0(0):. 2021-03-01
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Establishment of MHHi001-A-5, a GCaMP6f and RedStar dual reporter human iPSC line for in vitro and in vivo characterization and in situ tracing of iPSC derivatives
Authors: A Haase, T Kohrn, V Fricke, ME Ricci Sign, M Witte, G Göhring, I Gruh, U Martin
Stem Cell Research, 2021-01-30;52(0):102206. 2021-01-30
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The ER Unfolded Protein Response Effector, ATF6, Reduces Cardiac Fibrosis and Decreases Activation of Cardiac Fibroblasts
Authors: WT Stauffer, EA Blackwood, K Azizi, RJ Kaufman, CC Glembotski
Int J Mol Sci, 2020-02-18;21(4):. 2020-02-18
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Biosynthetic CircRNA_001160 induced by PTBP1 regulates the permeability of BTB via the CircRNA_001160/miR-195-5p/ETV1 axis
Authors: H Li, S Shen, X Ruan, X Liu, J Zheng, Y Liu, C Yang, D Wang, L Liu, J Ma, T Ma, P Wang, H Cai, Z Li, L Zhao, Y Xue
Cell Death Dis, 2019-12-20;10(12):960. 2019-12-20
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Peptide hormone ELABELA enhances extravillous trophoblast differentiation, but placenta is not the major source of circulating ELABELA in pregnancy
Authors: D Georgiadou, S Boussata, WHM Ranzijn, LEA Root, S Hillenius, JM Bij de Weg, CNH Abheiden, MA de Boer, JIP de Vries, TGM Vrijkotte, CB Lambalk, EAM Kuijper, GB Afink, M van Dijk
Sci Rep, 2019-12-13;9(1):19077. 2019-12-13
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Development of a Gut-On-A-Chip Model for High Throughput Disease Modeling and Drug Discovery
Authors: C Beaurivage, E Naumovska, YX Chang, ED Elstak, A Nicolas, H Wouters, G van Moolen, HL Lanz, SJ Trietsch, J Joore, P Vulto, RAJ Janssen, KS Erdmann, J Stallen, D Kurek
Int J Mol Sci, 2019-11-12;20(22):. 2019-11-12
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Circular RNA USP1 regulates the permeability of blood-tumour barrier via miR-194-5p/FLI1 axis
Authors: Y Gao, P Wu, Y Ma, Y Xue, Y Liu, J Zheng, X Liu, Q He, J Ma, L Liu, P Wang
J. Cell. Mol. Med., 2019-10-26;0(0):. 2019-10-26
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Zika virus enhances monocyte adhesion and transmigration favoring viral dissemination to neural cells
Authors: NV Ayala-Nune, G Follain, F Delalande, A Hirschler, E Partiot, GL Hale, BC Bollweg, J Roels, M Chazal, F Bakoa, M Carocci, S Bourdoulou, O Faklaris, SR Zaki, A Eckly, B Uring-Lamb, F Doussau, S Cianferani, C Carapito, FMJ Jacobs, N Jouvenet, JG Goetz, R Gaudin
Nat Commun, 2019-09-27;10(1):4430. 2019-09-27
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Discovery of endoplasmic reticulum calcium stabilizers to rescue ER-stressed podocytes in nephrotic syndrome
Authors: SJ Park, Y Kim, SM Yang, MJ Henderson, W Yang, M Lindahl, F Urano, YM Chen
Proc. Natl. Acad. Sci. U.S.A., 2019-06-24;0(0):. 2019-06-24
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Tumour-treating fields (TTFields): Investigations on the mechanism of action by electromagnetic exposure of cells in telophase/cytokinesis
Authors: L Berkelmann, A Bader, S Meshksar, A Dierks, G Hatipoglu, JK Krauss, K Schwabe, D Manteuffel, A Ngezahayo
Sci Rep, 2019-05-14;9(1):7362. 2019-05-14
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Formation of three-dimensional tubular endothelial cell networks under defined serum-free cell culture conditions in human collagen hydrogels
Authors: B Andrée, H Ichanti, S Kalies, A Heisterkam, S Strau beta, PM Vogt, A Haverich, A Hilfiker
Sci Rep, 2019-04-01;9(1):5437. 2019-04-01
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Randomly Distributed K14+ Breast Tumor Cells Polarize to the Leading Edge and Guide Collective Migration in Response to Chemical and Mechanical Environmental Cues
Authors: PY Hwang, A Brenot, AC King, GD Longmore, SC George
Cancer Res., 2019-03-12;79(8):1899-1912. 2019-03-12
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Interaction of Treponema pallidum, the syphilis spirochete, with human platelets
Authors: B Church, E Wall, JR Webb, CE Cameron
PLoS ONE, 2019-01-18;14(1):e0210902. 2019-01-18
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Mechanism of piR-DQ590027/MIR17HG regulating the permeability of glioma conditioned normal BBB
Authors: X Leng, J Ma, Y Liu, S Shen, H Yu, J Zheng, X Liu, L Liu, J Chen, L Zhao, X Ruan, Y Xue
J. Exp. Clin. Cancer Res., 2018-10-11;37(1):246. 2018-10-11
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Increased macrophages and�changed brain endothelial cell gene expression in the frontal cortex of people with schizophrenia displaying inflammation
Authors: HQ Cai, VS Catts, MJ Webster, C Galletly, D Liu, M O'Donnell, TW Weickert, CS Weickert
Mol. Psychiatry, 2018-09-13;0(0):. 2018-09-13
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Smooth muscle cells differentiated from human mesenchymal stem cells regulated by microRNA (miR)-503 and miR-222-5p are suitable for vascular tissue engineering
Authors: W Gu, X Hong, A Le Bras, WN Nowak, S Issa Bhalo, J Deng, Y Xie, Y Hu, XZ Ruan, Q Xu
J. Biol. Chem., 2018-04-11;0(0):. 2018-04-11
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Protein S and Gas6 induce efferocytosis of HIV-1-infected cells
Authors: BA Chua, JA Ngo, K Situ, CM Ramirez, H Nakano, K Morizono
Virology, 2018-01-02;515(0):176-190. 2018-01-02
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Metabolite Profile and Antiproliferative Effects in HaCaT Cells of a Salix reticulata Extract.
Authors: Corradi E, Schmidt N, Raber N, De Mieri M, Hamburger M, Butterweck V, Potterat O
Planta Med, 2017-04-27;83(14):1149-1158. 2017-04-27
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Enhanced transport of plant-produced rabies single chain antibody-RVG peptide fusion protein across an in cellulo blood-brain barrier device
Authors: W Phoolcharo, C Prehaud, CJ van Dollew, L Both, A da Costa, M Lafon, JK Ma
Plant Biotechnol. J, 2017-04-19;0(0):. 2017-04-19
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Knockdown of long non-coding RNA XIST increases blood-tumor barrier permeability and inhibits glioma angiogenesis by targeting miR-137
Authors: H Yu, Y Xue, P Wang, X Liu, J Ma, J Zheng, Z Li, Z Li, H Cai, Y Liu
Oncogenesis, 2017-03-13;6(3):e303. 2017-03-13
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PVT1 affects growth of glioma microvascular endothelial cells by negatively regulating miR-186
Authors: Y Ma, P Wang, Y Xue, C Qu, J Zheng, X Liu, J Ma, Y Liu
Tumour Biol., 2017-03-01;39(3):1010428317694. 2017-03-01
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Multifunctional LUV liposomes decorated for BBB and amyloid targeting. A. In vitro proof-of-concept
Authors: K Papadia, E Markoutsa, S Mourtas, AD Giannou, B La Ferla, F Nicotra, M Salmona, P Klepetsani, GT Stathopoul, SG Antimisiar
Eur J Pharm Sci, 2017-02-11;101(0):140-148. 2017-02-11
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Trojan Horse Transit Contributes to Blood-Brain Barrier Crossing of a Eukaryotic Pathogen
Authors: FH Santiago-T, MD Onken, JA Cooper, RS Klein, TL Doering
MBio, 2017-01-31;8(1):. 2017-01-31
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Mesencephalic Astrocyte-Derived Neurotrophic Factor as a Urine Biomarker for Endoplasmic Reticulum Stress-Related Kidney Diseases.
Authors: Kim Y, Lee H, Manson S, Lindahl M, Evans B, Miner J, Urano F, Chen Y
J Am Soc Nephrol, 2016-03-03;27(10):2974-2982. 2016-03-03
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Maspin Expression in Prostate Tumor Cells Averts Stemness and Stratifies Drug Sensitivity.
Authors: Bernardo M, Kaplun A, Dzinic S, Li X, Irish J, Mujagic A, Jakupovic B, Back J, Van Buren E, Han X, Dean I, Chen Y, Heath E, Sakr W, Sheng S
Cancer Res, 2015-07-24;75(18):3970-9. 2015-07-24
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DOCK8 regulates lymphocyte shape integrity for skin antiviral immunity.
Authors: Zhang, Qian, Dove, Christop, Hor, Jyh Lian, Murdock, Heardley, Strauss-Albee, Dara M, Garcia, Jordan A, Mandl, Judith N, Grodick, Rachael, Jing, Huie, Chandler-Brown, Devon B, Lenardo, Timothy, Crawford, Greg, Matthews, Helen F, Freeman, Alexandr, Cornall, Richard, Germain, Ronald N, Mueller, Scott N, Su, Helen C
J Exp Med, 2014-11-24;211(13):2549-66. 2014-11-24
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MiR-18a increased the permeability of BTB via RUNX1 mediated down-regulation of ZO-1, occludin and claudin-5.
Authors: Miao Y, Zhao Y, Zhao L, Wang P, Liu Y, Ma J, Xue Y
Cell Signal, 2014-10-29;27(1):156-67. 2014-10-29
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Biphasic modulation of paracellular claudin-5 expression in mouse brain endothelial cells is mediated through the phosphoinositide-3-kinase/AKT pathway.
Authors: Camire R, Beaulac H, Brule S, McGregor A, Lauria E, Willis C
J Pharmacol Exp Ther, 2014-10-03;351(3):654-62. 2014-10-03
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RPTPalpha controls epithelial adherens junctions, linking E-cadherin engagement to c-Src-mediated phosphorylation of cortactin.
Authors: Truffi M, Dubreuil V, Liang X, Vacaresse N, Nigon F, Han S, Yap A, Gomez G, Sap J
J Cell Sci, 2014-03-20;127(0):2420-32. 2014-03-20
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Glycosylation at Asn211 regulates the activation state of the discoidin domain receptor 1 (DDR1).
Authors: Fu H, Valiathan R, Payne L, Kumarasiri M, Mahasenan K, Mobashery S, Huang P, Fridman R
J Biol Chem, 2014-02-07;289(13):9275-87. 2014-02-07
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Jab1 regulates Schwann cell proliferation and axonal sorting through p27.
Authors: Porrello, Emanuela, Rivellini, Cristina, Dina, Giorgia, Triolo, Daniela, Del Carro, Ubaldo, Ungaro, Daniela, Panattoni, Martina, Feltri, Maria La, Wrabetz, Lawrence, Pardi, Ruggero, Quattrini, Angelo, Previtali, Stefano
J Exp Med, 2013-12-16;211(1):29-43. 2013-12-16
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Transcellular targeting of fiber- and hexon-modified adenovirus vectors across the brain microvascular endothelial cells in vitro.
Authors: Laakkonen, Johanna, Engler, Tatjana, Romero, Ignacio, Weksler, Babette, Couraud, Pierre-O, Kreppel, Florian, Kochanek, Stefan
PLoS ONE, 2012-09-27;7(9):e45977. 2012-09-27
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Targeted protein engineering provides insights into binding mechanism and affinities of bacterial collagen adhesins.
Authors: Ross C, Liang X, Liu Q, Murray B, Hook M, Ganesh V
J Biol Chem, 2012-08-03;287(41):34856-65. 2012-08-03
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Characterization of the interface between normal and transformed epithelial cells.
Authors: Hogan C, Dupre-Crochet S, Norman M, Kajita M, Zimmermann C, Pelling AE, Piddini E, Baena-Lopez LA, Vincent JP, Itoh Y, Hosoya H, Pichaud F, Fujita Y
Nat. Cell Biol., 2009-03-15;11(4):460-7. 2009-03-15
FAQs
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What is the difference between the standard Cultrex Rat Collagen I and the Cultrex 3-D Rat Collagen I?
Cultrex 3-D Rat Collagen I undergoes the same basic purification and efficacy tests as the standard collagen. However, it undergoes additional 3-D culture validation; it has been tested extensively for the ability to promote growth and differentiation of cell types, visualized by morphology in three dimensions in vitro.
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What are the differences between Cultrex Rat Collagen I and Cultrex Rat Collagen I, Low Viscosity?
Cultrex Rat Collagen I is provided at 5 mg/mL and is not pepsin treated, so it has intact telopeptides and is highly viscous. Cultrex Rat Collagen I, Low Viscosity is provided at 3 mg/mL, and it is easier to pipet. Both are provided in acetic acid and require pH neutralization prior to use. Please consult the provided product data sheets for pH neutralization instructions.
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Is Cultrex Rat Collagen I atelocollagen or telocollagen?
Cultrex Rat Collagen I is telocollagen.
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After coating the Cultrex Rat Collagen I (catalog # 3440-100-01), there is a white film on the plate. What is this white film? Is this normal?
This white film is normal. The collagen coating can result in a slight white haziness to the plate, which could also be the result of excess salt if the plates are not well aspirated.
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Can the Organoid Harvesting Solution, Catalog # 3700-100-01, be used to dissociate cells grown on a substrate of Cultrex Rat Collagen 1?
The Organoid Harvesting Solution works well with BME (Basement Membrane Extract). For cells grown on Cultrex Rat Collagen 1, the recommended enzyme to use to dissociate cells is Collagenase. Collagenase treatment at 37oC for 30 minutes is recommended.
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