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DAF FM diacetate

Catalog #: 7756 Datasheet / COA / SDS
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DAF FM diacetate | CAS No. 254109-22-3 | Fluorescent Cell Indicators and Sensors
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Description: Cell permeable photostable nitric oxide (NO) fluorescent indicator

Chemical Name: 4-Amino-5-methylamino-2',7'-difluorofluorescein diacetate

Purity: ≥80%

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Biological Activity

DAF-FM DA is a cell-permeable fluorescent probe for the detection of intracellular nitric oxide (NO). Once inside the cell, it is hydrolyzed by cytosolic esterases to cell-impermeant DAF-FM. DAF-FM reacts with NO+ equivalents, such as nitric anhydride (N2O3), which are formed by autoxidation of NO. Under aerobic conditions, DAF-FM can rapidly and irreversibly trap NO to yield highly fluorescent triazolofluoresceins (DAF-Ts). DAF-FM DA is photostable and stable in pH 5.5 to 12. It can be used to quantitatively monitor agonist-evoked NO synthesis in cultured HUVECs. DAF-FM DA may also be used to quantify NO levels in induced pluripotent stem cell-derived derived cardiomyocytes (iPSC-CM).

It is recommended to prepare stock solutions in DMSO.

Optical Data for DAF FM diacetate

DAF FM diacetate Dye Spectra
Emission Color Green
λabs 495 nm
λem 515 nm
Extinction Coefficient (ε) 84000 M-1cm-1
Cell Permeable Yes
pH Sensitivity Independent of pH when pH >5.8
Application Confocal Microscopy, Flow cytometry

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Technical Data

M.Wt:
496.42
Formula:
C25H18F2N2O7
Purity:
≥80%
Storage:
Store at -20°C
CAS No:
254109-22-3

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

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