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Donkey Anti-Mouse IgG Biotinylated Antibody

Catalog #: BAF018
3 Citations Datasheet / COA / SDS
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BAF018
R&D Systems Antibodies
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Citations (3)
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Donkey Anti-Mouse IgG Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IgG1, IgG2A, IgG2B, and IgG3 heavy and light chains in direct ELISA and Western blots. In direct ELISA, less than 5% cross‑reactivity with human IgG, rabbit IgG, and chicken IgY is observed.
Source
Polyclonal Donkey IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse IgG
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
Use at a 1:2000 dilution
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Citations for Donkey Anti-Mouse IgG Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Elastase as a potential biomarker for radiation-induced gut wall injury of the distal bowel in an experimental mouse model
    Authors: F Sjöberg, DK Malipatlol, P Patel, U Wilderäng, M Kalm, G Steineck, C Bull
    Acta Oncol, 2018-02-15;0(0):1-6.
    Species: Mouse
    Sample Types: Fecal Extracts
    Applications: ELISA Development (Secondary)
  2. Expression and prognostic significance of CTBP2 in human gliomas
    Oncol Lett, 2016-08-11;12(4):2429-2434.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Microneedle patch for the ultrasensitive quantification of protein biomarkers in interstitial fluid
    Authors: Zheyu Wang, Jingyi Luan, Anushree Seth, Lin Liu, Minli You, Prashant Gupta et al.
    Nature Biomedical Engineering

FAQs

  1. Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
    • All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.
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