Dopa Decarboxylase/DDC Antibody

Catalog #: PPS065 Datasheet / COA / SDS

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PPS065

Detection of Dopa Decarboxylase/DDC by Western Blot.

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Dopa Decarboxylase/DDC Antibody Summary

Species Reactivity

Human, Rat, Bovine, Canine, Guinea Pig, Rabbit, Sheep

Specificity

Human, bovine, canine, guinea pig, rabbit, rat, and sheep ~55 kDa DOPA Decarboxylase (DDC) in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Dopa Decarboxylase/DDC

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1:1000 dilution

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Dopa Decarboxylase/DDC by Western Blot. Western blot of bovine adrenal medulla lysate showing specific immunolabeling of the ~55 kDa DDC protein.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: Dopa Decarboxylase/DDC

DOPA decarboxylase (Aromatic-L-amino acid decarboxylase; also DDC) is a 54 kDa member of the alpha -family of pyroxidal phosphate enzymes. Functional DDC is a homodimeric, pyridoxal phosphate-dependent enzyme that calatyzes the decarboxylation of DOPA to dopamine, L-tryptophan to tryptamine, and 5-hydroxytryptophan to serotonin. Both dopamine and serotonin are important neurotransmitters. It also demonstrates deaminase activity, and is regulated by O2 availability. Bovine DDC is 487 amino acids (aa) in length and contains an N-terminal decarboxylase domain (aa 35-414) and a C-terminal pyroxidal binding site (L303). Bovine DDC is 88% and 86% aa identical to human and rat DDC, respectively.

References

Kang, U.J. and T.H. Joh (1990) Brain Res. Mol. Brain Res. 8:83.
Ichinose, H. et al. (1989) Biochem. Biophys. Res. Commun. 164:1024.
Moore, P.S. et al. (1996) Biochem. J. 315:249.
Bertoldi, M. and C.B. Voltattorni (2003) Biochim. Biophys. Acta 1647:42.

Entrez Gene IDs

1644 (Human)

Alternate Names

AADC; AADCaromatic-L-amino-acid decarboxylase; DDC; dopa decarboxylase (aromatic L-amino acid decarboxylase); Dopa Decarboxylase; EC 4.1.1; EC 4.1.1.28