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DYKDDDDK Epitope Tag Biotinylated Antibody

Catalog #: BAM8529 1 Review Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
BAM8529
Detection of DYKDDDDK (FLAG®) Epitope Tag by Western Blot.
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DYKDDDDK Epitope Tag Biotinylated Antibody Summary

Specificity
Detects N-terminal and C-terminal DYKDDDDK (FLAG®) epitope tagged proteins in Western blot.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1042E
Purification
Protein A or G purified from cell culture supernatant
Immunogen
DYKDDDDK epitope tag
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of DYKDDDDK (FLAG<sup>®</sup>) Epitope Tag antibody by Western Blot. View Larger

Detection of DYKDDDDK (FLAG®) Epitope Tag by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with C-terminal DYKDDDDK-tagged Abro1 and N-terminal DYKDDDDK-tagged RNF20. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-DYKDDDDK Biotinylated Monoclonal Antibody (Catalog # BAM8529) followed by Streptavidin-HRP (Catalog # DY998). Specific bands were detected for DYKDDDDK-tagged proteins at approximately 55 and 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Product Datasheets

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Product Specific Notices

FLAG® is a registered trademark of SigmaAldrich Co. LLC

FAQs

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DYKDDDDK Epitope Tag Biotinylated Antibody
By Anonymous on 03/18/2020
Application: Octet Binding Assay Sample Tested: Purified protein Species: Human

In an octet binding assay, streptavidin biosensors are able to capture the DYKDDDDK epitope tag biotinylated antibody. The DYKDDDDK epitope tag biotinylated antibody can then bind flag tagged proteins.

Other DYKDDDDK Epitope Tag Biotinylated Antibody BAM8529