E 64d
Chemical Name: (2S,3S)-3-[[[(1S)-3-Methyl-1-[[(3-methylbutyl)amino]carbonyl]butyl]amino]carbonyl]-2-oxiranecarboxylic acid ethyl ester
Purity: ≥98%
Biological Activity
E 64d is an inhibitor of cathepsins B and L; also thought to inhibit calpain. Inhibits lysosomal proteases and interferes with autolysosomal digestion when used in combination with pepstatin A (Cat. No. 1190). Lysosome and cell permeable. Also inhibits entry of SARS-Cov-2 (COVID-19) into Caco-2 and Vero cells in combination with Camostat mesylate (Cat.No. 3193) in vitro.Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Background References
-
Autophagy upregulation by inhibitors of caspase-3 and mTOR enhances radiotherapy in a mouse model of lung cancer.
Kim et al.
Autophagy, 2008;4:659 -
Autophagy is activated in colorectal cancer cells and contributes to the tolerance to nutrient deprivation.
Sato et al.
Cancer Res., 2007;67:9677 -
Lysosomal turnover, but not a cellular level, of endogenous LC3 is a marker for autophagy.
Tanada et al.
Autophagy, 2005;1:84 -
Inhibition of cysteine proteinases in lysosomes and whole cells.
Wilcox and Mason
Biochem.J., 1992;285:495 -
SARS-CoV-2 cell entry depends on ACE2 and TMPRSS2 and is blocked by a clinically-proven protease inhibitor.
Hoffmann et al.
Cell, 2020;
Product Datasheets
Reconstitution Calculator
Molarity Calculator
Citations for E 64d
The citations listed below are publications that use Tocris products. Selected citations for E 64d include:
6 Citations: Showing 1 - 6
-
Cell culture systems for isolation of SARS-CoV-2 clinical isolates and generation of recombinant virus.
Authors: Da-Yuan Et al.
iScience 2023;26:106634
-
SARS-CoV-2 spike N-terminal domain modulates TMPRSS2-dependent viral entry and fusogenicity.
Authors: Bo Et al.
Cell Rep 2022;40:111220
-
PD-L1 promotes myofibroblastic activation of hepatic stellate cells by distinct mechanisms selective for TGF-β receptor I versus II.
Authors: Arlene H Et al.
Cell Rep 2022;38:110349
-
A simple protocol to produce mature human-induced pluripotent stem cell-derived cardiomyocytes.
Authors: Yingqiong Et al.
STAR Protoc 2021;2:100912
-
TRPC5-induced autophagy promotes drug resistance in breast carcinoma via CaMKKβ/AMPKα/mTOR pathway.
Authors: Zhang Et al.
Sci Rep 2017;7:3158
-
Autophagosomal Content Profiling Reveals an LC3C-Dependent Piecemeal Mitophagy Pathway.
Authors: Guerroue Et al.
Mol Cell 2017;68:786
FAQs
No product specific FAQs exist for this product, however you may
View all Small Molecule FAQsReviews for E 64d
There are currently no reviews for this product. Be the first to review E 64d and earn rewards!
Have you used E 64d?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
