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Endothelin-1 Quantikine ELISA Kit

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Multi-species Endothelin-1 ELISA Standard Curve
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Summary Sample Values Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Endothelin-1 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (75 uL), Serum (75 uL), EDTA Plasma (75 uL), Heparin Plasma (75 uL), Urine (75 uL)
Sensitivity
0.207 pg/mL
Assay Range
0.4 - 25 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Synthetic and natural Endothelin-1
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Sample Values

Serum/Plasma/Urine - Samples from apparently healthy volunteers were evaluated for the presence of Endothelin-1 in this assay. No medical histories were available for the donors used in this study.

Sample TypeMean (pg/mL)Range (pg/mL)Standard Deviation (pg/mL)
Human serum (n=35)1.240.47-2.000.35
Human heparin plasma (n=35)1.170.58-1.960.32
Mouse serum (n=14)3.101.95-4.030.58
Mouse EDTA plasma (n=15)2.821.96-3.680.45
Rat serum (n=15)1.631.32-2.070.20
Rat EDTA (n=15)1.590.53-2.360.44

Sample TypeMean of Detectable (pg/mL)% DetectableRange (pg/mL)
Human EDTA plasma (n=35)1.1797ND-1.92
Human urine (n=25)0.724480.44
ND=Non-detectable

Cell Culture Supernates:
Human peripheral blood leukocytes were cultured in DMEM supplemented with 5% fetal bovine serum, 50 μM beta -mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. The cells were cultured unstimulated or stimulated with 10 μg/mL PHA for 1 and 6 days. Aliquots of the cell culture supernates were removed and assayed for levels of Endothelin-1.

ConditionDay 1 (pg/mL)Day 6 (pg/mL)
UnstimulatedND1.29
Stimulated3.467.33
ND=Non-detectable

Tissue from mice or rats were homogenized and seeded into 100 mL of RPMI containing 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate for the indicated times. Aliquots of the cell culture supernates were removed and assayed for levels of Endothelin-1.

Sample TypeObserved Levels (pg/mL)
Mouse liver (3 days)1.05
Mouse lung (1 day)5.15
Rat lung (1 Day)20.8





Product Summary

The Quantikine Endothelin-1 immunoassay is a 4.5 hour solid phase ELISA designed to measure ET-1 in cell culture supernates, serum, plasma, and urine. It contains synthetic ET-1 and antibodies raised against synthetic ET-1. This immunoassay has been shown to accurately quantitate synthetic and naturally occurring ET-1.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 3 7.34 14.7 3.05 7.43 14.4
Standard Deviation 0.12 0.17 0.28 0.231 0.438 0.759
CV% 4 2.3 1.9 7.6 5.9 5.3

Recovery

The recovery of Endothelin-1 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 99 88-108
Human EDTA Plasma (n=4) 93 87-102
Human Heparin Plasma (n=4) 93 85-106
Human Serum (n=4) 98 86-107
Mouse EDTA Plasma (n=4) 99 102-107
Mouse Serum (n=4) 94 86-104
Rat EDTA Plasma (n=4) 99 86-113
Rat Serum (n=4) 98 88-113
Urine (n=4) 91 85-109

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of Endothelin-1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Multi-species Endothelin-1 ELISA Linearity

Scientific Data

N/A Multi-species Endothelin-1 ELISA Standard Curve View Larger

Multi-species Endothelin-1 ELISA Standard Curve

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Endothelin-1

Endothelin-1 (ET-1), a peptide of 21 amino acid (aa) residues, is a pleiotropic molecule best known for its action as a potent vasoconstrictor (1). Originally isolated from porcine aortic endothelial cells, ET-1 is one of a family of three proteins encoded by distinct genes that also includes Endothelin-2 (ET-2) and Endothelin-3 (ET-3) (2, 3). ET-2 and ET-3 differ from ET-1 by 2 and 6 amino acids, respectively (1, 2). All members of the Endothelin family contain two essential disulfide bridges and six conserved aa residues at the C-terminus. Human ET-1 is initially synthesized as a pre-pro-polypeptide of 212 amino acids (2, 4). It is proteolytically cleaved by a signal peptidase to produce pro-ET-1 and further processed by a Furin-like protease to yield a 38 aa peptide termed Big ET-1 (5, 6). Big ET-1 is then cleaved by the membrane-bound metalloprotease Endothelin-converting enzyme (ECE-1), producing the potent 21 aa mature form ET-1 (aa 1-21) (7, 8). Alternatively, ET-1 may exist in an active 31 aa form (ET-1 (aa 1-31)) following cleavage of Big ET-1 by chymase (9-12). The vascular endothelium is an abundant source of ET-1 (3, 13). It may also be expressed by leukocytes, smooth muscle cells, mesangial cells, cardiac myocytes, and astrocytes (14, 15). ET-1 can be induced in endothelial cells by many factors including mechanical stimulation, various hormones, and pro-inflammatory cytokines (16). Production is inhibited by nitric oxide (NO), Prostacyclin, and atrial natriuretic peptide (ANP) (17-19). 

Two receptors for the Endothelin family have been cloned and designated ETA and ETB (20-23). ETA and ETB belong to the large family of heptahelical G protein-coupled receptors. The ETA receptor shows a higher affinity for ET-1 than for ET-2 and lowest affinity for ET-3, while the ETB receptor shows approximately equal affinity for each of the three Endothelins (21, 22, 24). ETA is primarily responsible for the vasoconstrictor effects of ET-1 and is expressed by blood vessel smooth muscle cells (25, 26). The ETB receptor is also present in smooth muscle and the endothelia of blood vessels, kidney, lung, and brain (27). ET-1 has the ability to activate an array of signaling cascades including classical phosphatidylinositol turnover pathways leading to downstream PKC activation and Ca2+ mobilization (28-32). Other potential signaling mediators activated or produced by ET-1 include PI 3-kinase/Akt, NO, FAK, and Rho GTPases (32-37). ET-1 signaling may also be mediated indirectly via transactivation of the EGF receptor leading to downstream signaling by Ras and MAP kinases (38, 39). Injection of a single dose of ET-1 produces an initial decrease in systemic blood pressure followed by a prolonged increase in blood pressure (16, 40, 41). Blockade of Endothelin receptors with a systemic injection of an ETA/ETB antagonist causes progressive vasodilation, and elevated levels of ET-1 are found in some forms of human hypertension (42, 43). ET-1 also stimulates cardiac contraction and the growth of cardiac myocytes, regulates the release of vasoactive substances, and stimulates smooth muscle cell mitogenesis (32, 44-46). It also acts as a pro-survival factor for endothelial cells and regulates secretion by hypothalamic and pituitary cells (47, 48). ET-1 may control inflammatory responses by promoting the adhesion and migration of neutrophils and stimulating the production of pro-inflammatory cytokines (49-53). It has also been implicated in cancer progression at several levels including regulating the proliferation and migration of tumor cells and acting as a pro-angiogenic factor (54, 55). In addition, ET-1 has putative roles in other pathologies including septic shock, atherosclerosis, heart failure, renal insufficiency, pulmonary hypertension, and cerebrovascular conditions associated with subarachnoid hemorrhage (15, 56-63).

Entrez Gene IDs:
1906 (Human); 13614 (Mouse); 24323 (Rat)
Alternate Names:
ARCND3; EDN1; endothelin 1; Endothelin-1; ET1; HDLCQ7; PPET1; preproendothelin-1; QME
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 150 µL Assay Diluent
  3.   Add 150 µL of Assay Diluent to each well.
    4. 75 µL Standard, Control, or Sample
  4.   Add 75 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 1 hour on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 3 hours on the shaker.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Endothelin-1 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

84 Citations: Showing 1 - 10
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  1. Effect of endothelin-1 on the blood pressure response to acute hypoxia and hyperoxia in healthy young men
    Authors: Gonsalves, AM;Baker, SE;Jacob, DW;Harper, JL;Manrique-Acevedo, CM;Limberg, JK;
    Physiological reports
    Species: Human
    Sample Types: Plasma
  2. Circulating Endothelin 1 but Not Transforming Growth Factor-? Levels Are Reduced after Pulmonary Endarterectomy in Subjects Affected by Chronic Thromboembolic Pulmonary Hypertension: A Prospective Cohort Study
    Authors: Totaro, P;Tirelli, C;De Amici, M;Grosjean, F;Testa, G;Sacchi, L;De Silvestri, A;Alloni, A;Kushta, E;Albertini, R;Rampino, T;D'Armini, AM;
    Journal of clinical medicine
    Species: Human
    Sample Types: Plasma
  3. Autophagy and ubiquitin-dependent proteolysis processes in left ventricular mass loss in pulmonary arterial hypertension
    Authors: Ho?da, MK;Ra?ny, U;Sordyl, M;Góralska, J;Kapusta, M;S?owi?ska-Solnica, K;Wojtysiak, D;Lis, G;Solnica, B;Kope?, G;Ho?da, J;
    Scientific reports
    Species: Rat
    Sample Types: Plasma
  4. Association of circulating biomarkers with illness severity measures differentiates myalgic encephalomyelitis/chronic fatigue syndrome and post-COVID-19 condition: a prospective pilot cohort study
    Authors: Domingo, JC;Battistini, F;Cordobilla, B;Zaragozá, MC;Sanmartin-Sentañes, R;Alegre-Martin, J;Cambras, T;Castro-Marrero, J;
    Journal of translational medicine
    Species: Human
    Sample Types: Plasma
  5. Pyruvate kinase M2 sustains cardiac mitochondrial integrity in septic cardiomyopathy by regulating PHB2-dependent mitochondrial biogenesis
    Authors: Ren, J;Ren, B;Fu, T;Ma, Y;Tan, Y;Zhang, S;Li, Y;Wang, Q;Chang, X;Tong, Y;
    International journal of medical sciences
    Species: Mouse
    Sample Types: Cell Culture Supernates
  6. Adipokines and Inflammatory Markers in Acute Myocardial Infarction Patients with and without Obstructive Sleep Apnea: A Comparative Analysis
    Authors: Vega-Jasso, AL;Amezcua-Guerra, LM;González-Pacheco, H;Sandoval-Zárate, J;González-Díaz, CA;Escobar-Alvarado, J;Manzano-Luna, JD;Brianza-Padilla, M;
    International journal of molecular sciences
    Species: Human
    Sample Types: Serum
  7. The Role of Polymorphism in the Endothelial Homeostasis and Vitamin D Metabolism Genes in the Severity of Coronary Artery Disease
    Authors: Ponasenko, A;Sinitskaya, A;Sinitsky, M;Khutornaya, M;Barbarash, O;
    Biomedicines
    Species: Human
    Sample Types: Whole Blood
  8. E-Selectin, ICAM-1, and ET-1 Biomarkers Address the Concern of the Challenging Diagnosis of Interstitial Lung Disease in Patients with Autoimmune Diseases
    Authors: Pulito-Cueto, V;Remuzgo-Martínez, S;Genre, F;Atienza-Mateo, B;Mora-Cuesta, VM;Iturbe-Fernández, D;Lera-Gómez, L;Mora-Gil, MS;Portilla, V;Corrales, A;Blanco, R;Cifrián, JM;González-Gay, MA;López-Mejías, R;
    International journal of molecular sciences
    Species: Human
    Sample Types: Serum
  9. Skin Temperature Circadian Rhythms and Dysautonomia in Myalgic Encephalomyelitis/Chronic Fatigue Syndrome: The Role of Endothelin-1 in the Vascular Tone Dysregulation
    Authors: T Cambras, MF Zerón-Ruge, A Díez-Nogue, MC Zaragozá, JC Domingo, R Sanmartin-, J Alegre-Mar, J Castro-Mar
    International Journal of Molecular Sciences, 2023-03-02;24(5):.
    Species: Human
    Sample Types: Plasma
  10. Endothelin-1 as a Biomarker of Idiopathic Pulmonary Fibrosis and Interstitial Lung Disease Associated with Autoimmune Diseases
    Authors: V Pulito-Cue, F Genre, R López-Mejí, VM Mora-Cuest, D Iturbe-Fer, V Portilla, M Sebastián, JG Ocejo-Viny, O Gualillo, R Blanco, A Corrales, I Ferraz-Ama, S Castañeda, JM Cifrián Ma, B Atienza-Ma, S Remuzgo-Ma, MÁ González-G
    International Journal of Molecular Sciences, 2023-01-09;24(2):.
    Species: Human
    Sample Types: Serum
  11. Targeting tumor-stroma communication by blocking endothelin-1 receptors sensitizes high-grade serous ovarian cancer to PARP inhibition
    Authors: P Tocci, C Roman, R Sestito, V Di Castro, A Sacconi, I Molineris, F Paolini, M Carosi, G Tonon, G Blandino, A Bagnato
    Cell Death & Disease, 2023-01-05;14(1):5.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Serum biomarkers, including nitric oxide metabolites (NOx), for prognosis of cardiovascular death and acute myocardial infarction in an ESSE-RF case-control cohort with 6.5-year follow up
    Authors: NG Gumanova, NL Bogdanova, VA Metelskaya, VI Tarasov, AY Kots, VA Kutsenko, AV Kontsevaya, OM Drapkina
    Scientific Reports, 2022-10-28;12(1):18177.
    Species: Human
    Sample Types: Serum
  13. Fibrocytes boost tumor-supportive phenotypic switches in the lung cancer niche via the endothelin system
    Authors: A Weigert, X Zheng, A Nenzel, K Turkowski, S Günther, E Strack, E Sirait-Fis, E Elwakeel, IM Kur, VS Nikam, C Valasaraja, H Winter, A Wissgott, R Voswinkel, F Grimminger, B Brüne, W Seeger, SS Pullamsett, R Savai
    Nature Communications, 2022-10-14;13(1):6078.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Serum Collected from Preeclamptic Pregnancies Drives Vasoconstriction of Human Omental Arteries-A Novel Ex Vivo Model of Preeclampsia for Therapeutic Development
    Authors: BR Fato, N de Alwis, S Beard, NK Binder, N Pritchard, S Tong, TJ Kaitu'u-Li, NJ Hannan
    International Journal of Molecular Sciences, 2022-09-16;23(18):.
    Species: Human
    Sample Types: Serum
  15. Endothelial dysfunction: The possible link between cardiovascular comorbidities and phenomenon of inflammaging from COPD
    Authors: E Tudorache, O Fira-Mladi, D Traila, M Marc, RM Rajnoveanu, DE Tofolean, AP Fildan
    Medicine, 2022-08-19;101(33):e30078.
    Species: Human
    Sample Types: Serum
  16. Cerebral blood flow is associated with markers of neurodegeneration in Huntington's disease
    Authors: NP Rocha, O Charron, GD Colpo, LB Latham, JE Patino, EF Stimming, L Freeman, AL Teixeira
    Parkinsonism & related disorders, 2022-08-06;102(0):79-85.
    Species: Human
    Sample Types: Plasma
  17. Actions of Esomeprazole on the Maternal Vasculature in Lean and Obese Pregnant Mice with Impaired Nitric Oxide Synthesis: A Model of Preeclampsia
    Authors: N de Alwis, NK Binder, YTM Mangwiro, S Beard, N Pritchard, E Kadife, BR Fato, E Keenan, FC Brownfoot, TJ Kaitu'u-Li, NJ Hannan
    International Journal of Molecular Sciences, 2022-07-25;23(15):.
    Species: Mouse
    Sample Types: Serum
  18. Elevated endothelin-1 levels as risk factor for an impaired ocular blood flow measured by OCT-A in glaucoma
    Authors: C Lommatzsch, K Rothaus, L Schopmeyer, M Feldmann, D Bauer, S Grisanti, C Heinz, M Kasper
    Scientific Reports, 2022-07-12;12(1):11801.
    Species: Human
    Sample Types: Plasma
  19. Plasma Endothelial and Oxidative Stress Biomarkers Associated with Late Mortality in Hospitalized COVID-19 Patients
    Authors: A Orea-Tejad, C Sánchez-Mo, OG Aztatzi-Ag, MP Sierra-Var, D González-I, Y Debray-Gar, MS Ortega-Rom, C Keirns-Dav, L Cornejo-Co, J Aguilar-Me
    Journal of Clinical Medicine, 2022-07-07;11(14):.
    Species: Human
    Sample Types: Plasma
  20. Serum and Vascular Stiffness Biomarkers Associated with the Severity of Degenerative Aortic Valve Stenosis and Cardiovascular Outcomes
    Authors: J Baran, ? Niewiara, J Podolec, M Siedli?ski, E Józefczuk, A Bernacik, R Badacz, T Przew?ocki, P Pieni??ek, K ?mudka, J Legutko, A Kab?ak-Zie
    Oncogene, 2022-06-17;9(6):.
    Species: Human
    Sample Types: Serum
  21. No association between intravenous fluid volume and endothelial glycocalyx shedding in patients undergoing resuscitation for sepsis in the emergency department
    Authors: S Macdonald, E Bosio, NI Shapiro, L Balmer, S Burrows, M Hibbs, T Jowitt, L Smart, G Arendts, D Fatovich
    Scientific Reports, 2022-05-24;12(1):8733.
    Species: Human
    Sample Types: Serum
  22. Elevated aqueous endothelin-1 concentrations in advanced diabetic retinopathy
    Authors: HM Kang, M Hasanuzzam, SW Kim, HJ Koh, SC Lee
    PLoS ONE, 2022-05-11;17(5):e0268353.
    Species: Human
    Sample Types: Aqueous Humor
  23. LncRNA MALAT1 functions as a biomarker of no-reflow phenomenon in ST-segment elevation myocardial infarction patients receiving primary percutaneous coronary intervention
    Authors: X Yang, R Dai, Z Qin, R Cai, Y Xu, Q Su
    Scientific Reports, 2022-02-28;12(1):3294.
    Species: Human
    Sample Types: Plasma
  24. Oxidative stress-induced endothelial dysfunction and decreased vascular nitric oxide in COVID-19 patients
    Authors: V Montiel, I Lobysheva, L Gérard, M Vermeersch, D Perez-Morg, T Castelein, JB Mesland, P Hantson, C Collienne, D Gruson, MA van Dievoe, A Persu, C Beauloye, M Dechamps, L Belkhir, A Robert, M Derive, PF Laterre, AHJ Danser, X Wittebole, JL Balligand
    EBioMedicine, 2022-02-23;77(0):103893.
    Species: Human
    Sample Types: Serum
  25. Classical prescription Dachuanxiong Formula delays nitroglycerin-induced pain response in migraine mice through reducing endothelin-1 level and regulating fatty acid biosynthesis
    Authors: CT Vong, Y Chen, Z Chen, C Gao, F Yang, S Wang, Y Wang
    Journal of ethnopharmacology, 2022-01-13;0(0):114992.
    Species: Mouse
    Sample Types: Plasma
  26. Differences in Endothelial Activation and Dysfunction Induced by Antiphospholipid Antibodies Among Groups of Patients With Thrombotic, Refractory, and Non-refractory Antiphospholipid Syndrome
    Authors: M Velásquez, LF Peláez, M Rojas, R Narváez-Sá, JA Velásquez, C Escudero, S San Martín, ÁP Cadavid
    Frontiers in Physiology, 2021-12-02;12(0):764702.
    Species: Human
    Sample Types: Cell Culture Supernates
  27. Andrographolide Attenuates Established Pulmonary Hypertension via Rescue of Vascular Remodeling
    Authors: X Nie, C Shen, J Tan, X Yang, W Wang, Y Dai, H Sun, Z Wu, J Chen
    Biomolecules, 2021-11-30;11(12):.
    Species: Human
    Sample Types: Cell Culture Supernates
  28. Diagnostic prediction model in subjects with low-risk unstable angina pectoris/non-ST segment Elevation Myocardial Infarction
    Authors: I Dakota, M Munawar, R Pranata, WM Raffaello, R Sukmawan
    European review for medical and pharmacological sciences, 2021-08-01;25(16):5145-5152.
    Species: Human
    Sample Types: Plasma
  29. Endothelin-1 as a Mediator of Heme Oxygenase-1-Induced Stemness in Colorectal Cancer: Influence of p53
    Authors: S Ríos-Arrab, JD Puentes-Pa, S Moreno-San, Á Szuba, J Casado, M García-Cos, J Escudero-F, M Verbeni, C Cano, C González-P, A Martín-Lag, Á Carazo, J León
    Journal of personalized medicine, 2021-06-04;11(6):.
    Species: Human
    Sample Types: Cell Culture Supernates
  30. Significant elevation of aqueous endothelin-1 in central retinal vein occlusion
    Authors: HM Kang, M Hasanuzzam, SW Kim, HJ Koh, SC Lee
    PLoS ONE, 2021-06-02;16(6):e0252530.
    Species: Human
    Sample Types: Aqueous Humor
  31. Syndecan-1 Promotes Angiogenesis in Triple-Negative Breast Cancer through the Prognostically Relevant Tissue Factor Pathway and Additional Angiogenic Routes
    Authors: E Nassar, N Hassan, EA El-Ghonaim, H Hassan, MS Abdullah, TV Rottke, L Kiesel, B Greve, SA Ibrahim, M Götte
    Cancers, 2021-05-12;13(10):.
    Species: Human
    Sample Types: Cell Culture Supernates
  32. Preoperative Serum GDF-15, Endothelin-1 Levels, and Intraoperative Factors as Short-Term Operative Risks for Patients Undergoing Cardiovascular Surgery
    Authors: T Kato, T Nakajima, T Fukuda, I Shibasaki, T Hasegawa, K Ogata, H Ogawa, S Hirota, H Ohashi, S Saito, Y Takei, M Tezuka, M Seki, T Kuwata, M Sakuma, S Abe, S Toyoda, T Inoue, H Fukuda
    Journal of Clinical Medicine, 2021-05-02;10(9):.
    Species: Human
    Sample Types: Serum
  33. Prolyl Endopeptidase contributes to early neutrophilic inflammation in acute cardiac transplant rejection
    Authors: GA Payne, NS Sharma, CV Lal, C Song, L Guo, C Margaroli, L Viera, S Kumar, J Li, M Bosley, D Xing, X Xu, JM Wells, JF George, JA Tallaj, M Leesar, JE Blalock, A Gaggar
    JCI Insight, 2021-03-22;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  34. Blood angiopoietin-2 predicts liver angiogenesis and fibrosis in hepatitis C patients
    Authors: Y Osawa, S Yoshio, Y Aoki, M Korenaga, M Imamura, T Oide, M Okawara, H Kawai, Y Tsutsui, Y Yoshida, S Yoshikawa, T Mori, T Yamazoe, T Kanto
    BMC gastroenterology, 2021-02-08;21(1):55.
    Species: Human
    Sample Types: Plasma
  35. Priapism in sickle cell disease: Associations between NOS3 and EDN1 genetic polymorphisms and laboratory biomarkers
    Authors: CVB Figueiredo, RP Santiago, CC da Guarda, RM Oliveira, LM Fiuza, SCMA Yahouédého, SP Carvalho, JSDS Neres, AMJ Oliveira, CA Fonseca, VML Nascimento, IM Lyra, MM Aleluia, MS Goncalves
    PLoS ONE, 2021-02-04;16(2):e0246067.
    Species: Human
    Sample Types: Serum
  36. Sorafenib not only impairs endothelium-dependent relaxation but also promotes vasoconstriction through the upregulation of vasoconstrictive endothelin type B receptors
    Authors: Q Yu, K Li, A Zhao, M Wei, Z Huang, Y Zhang, Y Chen, L Ting, W Chuan, L Xu, Y Zhang, C Xu, F Liu
    Toxicology and Applied Pharmacology, 2021-01-24;414(0):115420.
    Species: Rat
    Sample Types: Plasma
  37. Acute effects of leg heat therapy on walking performance and cardiovascular and inflammatory responses to exercise in patients with peripheral artery disease
    Authors: JC Monroe, Q Song, MS Emery, DM Hirai, RL Motaganaha, BT Roseguini
    Physiological Reports, 2021-01-01;8(24):e14650.
    Species: Human
    Sample Types: Plasma
  38. Acute Effects of Metformin and Vildagliptin after a Lipid-Rich Meal on Postprandial Microvascular Reactivity in Patients with Type 2 Diabetes and Obesity: A Randomized Trial
    Authors: A Schiappaca, PA Maranhão, MDGC Souza, DG Panazzolo, JF Nogueira N, E Bouskela, LG Kraemer-Ag
    J Clin Med, 2020-10-09;9(10):.
    Species: Human
    Sample Types: Plasma
  39. TGFBR3 Polymorphisms (rs1805110 and rs7526590) Are Associated with Laboratory Biomarkers and Clinical Manifestations in Sickle Cell Anemia
    Authors: RP Santiago, CVB Figueiredo, LM Fiuza, SCMA Yahouédého, RM Oliveira, MM Aleluia, SP Carvalho, CA Fonseca, VML Nascimento, LC Rocha, CC Guarda, MS Gonçalves
    Dis Markers, 2020-09-30;2020(0):8867986.
    Species: Human
    Sample Types: Serum
  40. Hepatic lipocalin 2 promotes liver fibrosis and portal hypertension
    Authors: J Chen, J Argemi, G Odena, MJ Xu, Y Cai, V Massey, A Parrish, R Vadigepall, J Altamirano, J Cabezas, P Gines, J Caballeria, N Snider, P Sancho-Bru, S Akira, I Rusyn, B Gao, R Bataller
    Scientific Reports, 2020-09-23;10(1):15558.
    Species: Human
    Sample Types: Serum
  41. Breast cancer diagnosis is associated with relative left ventricular hypertrophy and elevated endothelin-1 signaling
    Authors: ZH Maayah, S Takahara, AS Alam, M Ferdaoussi, G Sutendra, AOS El-Kadi, JR Mackey, E Pituskin, DI Paterson, JRB Dyck
    BMC Cancer, 2020-08-12;20(1):751.
    Species: Human
    Sample Types: Plasma
  42. Exercise-Induced Elevated BDNF Level Does Not Prevent Cognitive Impairment Due to Acute Exposure to Moderate Hypoxia in Well-Trained Athletes
    Authors: Z Piotrowicz, M Chalimoniu, K P?oszczyca, M Czuba, J Langfort
    Int J Mol Sci, 2020-08-04;21(15):.
    Species: Human
    Sample Types: Serum
  43. Guanine Deaminase in Human Epidermal Keratinocytes Contributes to Skin Pigmentation
    Authors: JM Jung, TK Noh, SY Jo, SY Kim, Y Song, YH Kim, SE Chang
    Molecules, 2020-06-05;25(11):.
    Species: Human
    Sample Types: Cell Culture Supernates
  44. Combination of Red Clover and Hops Extract Improved Menopause Symptoms in an Ovariectomized Rat Model
    Authors: MR Kim, HJ Kim, SH Yu, BS Lee, SY Jeon, JJ Lee, YC Lee
    Evid Based Complement Alternat Med, 2020-05-23;2020(0):7941391.
    Species: Rat
    Sample Types: Serum
  45. Sleep-disordered breathing does not impact maternal outcomes in women with hypertensive disorders of pregnancy
    Authors: DL Wilson, ME Howard, AM Fung, FJ O'Donoghue, M Barnes, M Lappas, SP Walker
    PLoS ONE, 2020-04-27;15(4):e0232287.
    Species: Human
    Sample Types: Plasma
  46. Urinary Biomarkers of Inflammation and Oxidative Stress Are Elevated in Obese Children and Correlate with a Marker of Endothelial Dysfunction
    Authors: V Selvaraju, P Ayine, M Fadamiro, JR Babu, M Brown, T Geetha
    Oxid Med Cell Longev, 2019-10-16;2019(0):9604740.
    Species: Human
    Sample Types: Urine
  47. Increased Levels of Endothelin-1 in Cerebrospinal Fluid Are a Marker of Poor Visual Recovery after Optic Neuritis in Multiple Sclerosis Patients
    Authors: M Castellazz, G Lamberti, MV Resi, E Baldi, LM Caniatti, G Galante, P Perri, M Pugliatti
    Dis. Markers, 2019-09-10;2019(0):9320791.
    Species: Human
    Sample Types: Serum
  48. Influence of bradykinin B2 receptor and dopamine D2 receptor on the oxidative stress, inflammatory response, and apoptotic process in human endothelial cells
    Authors: A Niewiarows, A Kozik, I Guevara-Lo
    PLoS ONE, 2018-11-14;13(11):e0206443.
    Species: Human
    Sample Types: Cell Culture Supernates
  49. Combination of Plasma Biomarkers and Clinical Data for the Detection of Myocardial Fibrosis or Aggravation of Heart Failure Symptoms in Heart Failure with Preserved Ejection Fraction Patients
    Authors: CK Wu, MM Su, YF Wu, JJ Hwang, LY Lin
    J Clin Med, 2018-11-08;7(11):.
    Species: Human
    Sample Types: Plasma
  50. Trans-repression of NF?B pathway mediated by PPAR? improves vascular endothelium insulin resistance
    Authors: Y Kong, Y Gao, D Lan, Y Zhang, R Zhan, M Liu, Z Zhu, G Zeng, Q Huang
    J. Cell. Mol. Med., 2018-11-05;0(0):.
    Species: Rat
    Sample Types: Serum
  51. Dysregulation of the endothelin pathway in lymphangioleiomyomatosis with no direct effect on cell proliferation and migration
    Authors: N Chebib, F Archer, A Bobet-Erny, C Leroux, V Cottin
    Sci Rep, 2018-10-02;8(1):14698.
    Species: Human
    Sample Types: Serum
  52. Mouse Mast Cell Protease 4 Deletion Protects Heart Function and Survival After Permanent Myocardial Infarction
    Authors: M Houde, A Schwertani, H Touil, L Desbiens, O Sarrhini, R Lecomte, M Lepage, H Gagnon, S Takai, G Pejler, D Jacques, F Gobeil, R Day, P D'Orléans-
    Front Pharmacol, 2018-08-31;9(0):868.
    Species: Mouse
    Sample Types: Tissue Homogenates
  53. Synergistic effect of renalase and chronic kidney disease on endothelin-1 in patients with coronary artery disease ? a cross-sectional study
    Authors: YH Li, WH Sheu, WJ Lee, J- Wang, CP Fu, KW Liang, IT Lee
    Sci Rep, 2018-05-09;8(1):7378.
    Species: Human
    Sample Types: Serum
  54. Adipose-Derived Stromal Vascular Fraction/Xenohybrid Bone Scaffold: An Alternative Source for Bone Regeneration
    Authors: I Roato, DC Belisario, M Compagno, L Verderio, A Sighinolfi, F Mussano, T Genova, F Veneziano, G Pertici, G Perale, R Ferracini
    Stem Cells Int, 2018-04-29;2018(0):4126379.
    Species: Human
    Sample Types: Cell Culture Supernates
  55. Canagliflozin inhibits interleukin-1?-stimulated cytokine and chemokine secretion in vascular endothelial cells by AMP-activated protein kinase-dependent and -independent mechanisms
    Authors: SJ Mancini, D Boyd, OJ Katwan, A Strembitsk, TA Almabrouk, S Kennedy, TM Palmer, IP Salt
    Sci Rep, 2018-03-27;8(1):5276.
    Species: Human
    Sample Types: Cell Culture Supernates
  56. Preeclamptic plasma stimulates the expression of miRNAs, leading to a decrease in endothelin-1 production in endothelial cells
    Authors: M Caldeira-D, MR Luizon, E Deffune, JE Tanus-Sant, PP Freire, RF Carvalho, H Bettiol, VC Cardoso, M Antonio Ba, RC Cavalli, VC Sandrim
    Pregnancy Hypertens, 2018-03-27;12(0):75-81.
    Species: Human
    Sample Types: Cell Culture Supernates
  57. Tumor characterization by ultrasound-release of multiple protein and microRNA biomarkers, preclinical and clinical evidence
    Authors: AL D'Souza, JR Chevillet, P Ghanouni, X Yan, M Tewari, SS Gambhir
    PLoS ONE, 2018-03-16;13(3):e0194268.
    Species: Human
    Sample Types: Plasma
  58. A Pecan-Rich Diet Improves Cardiometabolic Risk Factors in Overweight and Obese Adults: A Randomized Controlled Trial
    Authors: DL McKay, M Eliasziw, CYO Chen, JB Blumberg
    Nutrients, 2018-03-11;10(3):.
    Species: Human
    Sample Types: Serum
  59. Cardiac effects of fish oil in a rat model of streptozotocin-induced diabetes
    Authors: F Mayyas, R Jaradat, KH Alzoubi
    Nutr Metab Cardiovasc Dis, 2018-02-25;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
  60. The effect of low and high-intensity cycling in diesel exhaust on flow-mediated dilation, circulating NOx, endothelin-1 and blood pressure
    Authors: LV Giles, SJ Tebbutt, C Carlsten, MS Koehle
    PLoS ONE, 2018-02-21;13(2):e0192419.
    Species: Human
    Sample Types: Plasma
  61. Chronic ETAantagonist reverses hypertension and impairment of structure and function of peripheral small arteries in aortic stiffening
    Authors: X Guo, H Chen, L Han, S Haulon, GS Kassab
    Sci Rep, 2018-02-15;8(1):3076.
    Species: Rat
    Sample Types: Serum
  62. The Potential Therapeutic Role of Peroxisome ProliferatorActivated Receptors Agonist in Preeclamptic Pregnant Rats
    Authors: HIG Allam, AAA Masri
    J Coll Physicians Surg Pak, 2018-01-01;28(1):31-35.
    Species: Rat
    Sample Types: Plasma
  63. Influence of ethnic background on left atrial markers of inflammation, endothelial function and tissue remodelling
    Authors: CD Ruediger, B John, S Kumar, HS Lim, G Rangnekar, KC Roberts-Th, GD Young, D Chase, P Sanders, SR Willoughby
    Indian Pacing Electrophysiol J, 2017-08-30;18(1):1-5.
    Species: Human
    Sample Types: Serum
  64. Endothelin-1 receptor blockade as new possible therapeutic approach in multiple myeloma
    Authors: A Russignan, C Spina, N Tamassia, A Cassaro, A Rigo, A Bagnato, L Rosanò, A Bonalumi, M Gottardi, L Zanatta, A Giacomazzi, MT Scupoli, M Tinelli, U Salvadori, F Mosna, A Zamò, MA Cassatella, F Vinante, C Tecchio
    Br. J. Haematol., 2017-06-09;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  65. Preliminary study of hypoxia-related cardiovascular mediator-markers in patients with end-stage renal disease with and without diabetes and the effects of haemodialysis
    Authors: A Treweeke, J Hall, S Lambie, SJ Leslie, IL Megson, SM MacRury
    PLoS ONE, 2017-05-23;12(5):e0178171.
    Species: Human
    Sample Types: Plasma
  66. The endothelin B receptor plays a crucial role for the adhesion of neutrophils to the endothelium in sickle cell disease
    Authors: B Koehl, P Nivoit, W El Nemer, O Lenoir, P Hermand, C Pereira, V Brousse, L Guyonnet, G Ghinatti, M Benkerrou, Y Colin, C Le Van Kim, PL Tharaux
    Haematologica, 2017-04-06;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  67. Comparison of Serum Levels of Endothelin-1 in Chronic Periodontitis Patients Before and After Treatment
    Authors: W Khalid, SS Varghese, M Sankari, ND Jayakumar
    J Clin Diagn Res, 2017-04-01;11(4):ZC78-ZC81.
    Species: Human
    Sample Types: Serum
  68. Peripheral venous congestion causes time- and dose-dependent release of endothelin-1 in humans
    Authors: J Lin, N Chudasama, Y Hayashi, C Hawk, SD Ramnauth, KY Wong, A Harxhi, D Onat, M Wakabayash, N Uriel, UP Jorde, TH LeJemtel, HN Sabbah, RT Demmer, PC Colombo
    Physiol Rep, 2017-03-01;5(6):.
    Species: Human
    Sample Types: Plasma
  69. HMGB1 contributes to glomerular endothelial cell injury in ANCA-associated vasculitis through enhancing endothelium-neutrophil interactions
    Authors: C Wang, DY Chang, M Chen, MH Zhao
    J. Cell. Mol. Med, 2017-02-09;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates, Plasma
  70. Habitual cocoa intake reduces arterial stiffness in postmenopausal women regardless of intake frequency: a randomized parallel-group study
    Authors: Koichi Nakazato
    Clin Interv Aging, 2016-11-14;11(0):1645-1652.
    Species: Human
    Sample Types: Plasma
  71. Effects of equivalent sympathetic activation during hypoglycemia on endothelial function and pro-atherothrombotic balance in healthy individuals and obese standard treated type 2 diabetes
    Authors: Stephen N Davis
    Metab. Clin. Exp., 2016-09-12;65(12):1695-1705.
    Species: Human
    Sample Types: Plasma
  72. EDN1 Gene Variant is Associated with Neonatal Persistent Pulmonary Hypertension
    Sci Rep, 2016-07-18;6(0):29877.
    Species: Human
    Sample Types: Serum
  73. Cellular microbiology of Mycoplasma canis
    Authors: DL Michaels, JA Leibowitz, MT Azaiza, PK Shil, SM Shama, GF Kutish, SL Distelhors, MF Balish, MA May, DR Brown
    Infect Immun, 2016-05-24;0(0):.
    Species: Canine
    Sample Types: Cell Culture Supernates
  74. Chemical constituents and sources of ambient particulate air pollution and biomarkers of endothelial function in a panel of healthy adults in Beijing, China
    Authors: Shaowei Wu
    Sci. Total Environ, 2016-04-18;560(0):141-9.
    Species: Human
    Sample Types: Plasma
  75. Citreoviridin Enhances Atherogenesis in Hypercholesterolemic ApoE-Deficient Mice via Upregulating Inflammation and Endothelial Dysfunction.
    Authors: Hou H, Yuan N, Guo Q, Sun T, Li C, Liu J, Li Q, Jiang B
    PLoS ONE, 2015-05-01;10(5):e0125956.
    Species: Mouse
    Sample Types: Serum
  76. Tumor necrosis factor-alpha-induced reduction of glomerular filtration rate in rats with fulminant hepatic failure.
    Authors: Wang J, Wang D, Wang H, Wang Z, Wen Y, Sun C, Zhao Y, Wu J, Liu P
    Lab Invest, 2014-06-02;94(7):740-51.
    Species: Rat
    Sample Types: Serum
  77. Endothelin A receptor antagonism enhances inhibitory effects of anti-ganglioside GD2 monoclonal antibody on invasiveness and viability of human osteosarcoma cells.
    Authors: Liu B, Wu Y, Zhou Y, Peng D
    PLoS ONE, 2014-04-11;9(4):e93576.
    Species: Human
    Sample Types: Cell Culture Supernates
  78. Hydroxycarbamide reduces eosinophil adhesion and degranulation in sickle cell anaemia patients.
    Authors: Pallis F, Conran N, Fertrin K, Olalla Saad S, Costa F, Franco-Penteado C
    Br J Haematol, 2013-10-28;164(2):286-95.
    Species: Human
    Sample Types: Plasma
  79. TRPA1 controls inflammation and pruritogen responses in allergic contact dermatitis.
    Authors: Liu, Boyi, Escalera, Jasmine, Balakrishna, Shrilath, Fan, Lu, Caceres, Ana I, Robinson, Eve, Sui, Aiwei, McKay, M Craig, McAlexander, M Allen, Herrick, Christin, Jordt, Sven E
    FASEB J, 2013-05-30;27(9):3549-63.
    Species: Mouse
    Sample Types: Tissue Homogenates
  80. The mechanism through which octreotide inhibits hepatic stellate cell activity.
    Authors: Wang J, Wang L, Song G, Han B
    Mol Med Rep, 2013-03-20;7(5):1559-64.
    Species: Human
    Sample Types: Cell Culture Supernates
  81. Inhibitory effect of the combination therapy of simvastatin and pinocembrin on atherosclerosis in ApoE-deficient mice.
    Authors: Sang, Hui, Yuan, Na, Yao, Shutong, Li, Furong, Wang, Jiafu, Fang, Yongqi, Qin, Shucun
    Lipids Health Dis, 2012-12-05;11(0):166.
    Species: Mouse
    Sample Types: Serum
  82. Increased levels of inflammatory cytokines and endothelin-1 in alveolar macrophages from patients with chronic heart failure.
    Authors: Sikkeland LI, Dahl CP, Ueland T, Andreassen AK, Gude E, Edvardsen T, Holm T, Yndestad A, Gullestad L, Kongerud J, Aukrust P, ØIe E
    PLoS ONE, 2012-05-15;7(5):e36815.
    Species: Human
    Sample Types: Plasma
  83. Endothelin-1 induces endoplasmic reticulum stress by activating the PLC-IP(3) pathway: implications for placental pathophysiology in preeclampsia.
    Authors: Jain A, Olovsson M, Burton GJ, Yung HW
    Am. J. Pathol., 2012-04-12;180(6):2309-20.
    Species: Human
    Sample Types: Cell Culture Supernates
  84. The endothelin receptor antagonist bosentan improves peripheral endothelial function in patients with type 2 diabetes mellitus and microalbuminuria: a randomised trial.
    Authors: Rafnsson A, Bohm F, Settergren M, Gonon A, Brismar K, Pernow J
    Diabetologia, 2011-12-27;55(3):600-7.
    Species: Human
    Sample Types: Plasma

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Reviews for Endothelin-1 Quantikine ELISA Kit

Average Rating: 4.7 (Based on 9 Reviews)

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Endothelin-1 Quantikine ELISA Kit
By Anonymous on 12/15/2023
Sample Tested: rat plasma,rat serum

The kit worked excellent for rat plasma and serum.

Endothelin-1 Quantikine ELISA Kit
By Anonymous on 11/10/2023
Sample Tested: Plasma
Endothelin-1 Quantikine ELISA Kit DET100
Endothelin-1 Quantikine ELISA Kit
By Anonymous on 11/16/2022
Sample Tested: Plasma,Urine

Plasma was in range at 4-fold dilution. Urine was in range at 2-fold dilution.

Endothelin-1 Quantikine ELISA Kit DET100
Endothelin-1 Quantikine ELISA Kit
By Anonymous on 08/22/2019
Sample Tested: EDTA Plasma
Endothelin-1 Quantikine ELISA Kit DET100
Endothelin-1 Quantikine ELISA Kit
By Anonymous on 12/17/2018
Sample Tested: Urine
Endothelin-1 Quantikine ELISA Kit DET100
Endothelin-1 Quantikine ELISA Kit
By Christian Cassel on 10/11/2018
Sample Tested: Serum and Plasma
Endothelin-1 Quantikine ELISA Kit
By Anonymous on 09/11/2018
Sample Tested: Urine
Endothelin-1 Quantikine ELISA Kit DET100
Endothelin-1 Quantikine ELISA Kit
By Anonymous on 10/23/2017
Sample Tested: Plasma

Plasma was used from male mice.

Endothelin-1 Quantikine ELISA Kit DET100
Endothelin-1 Quantikine ELISA Kit
By Anonymous on 08/02/2017
Sample Tested: Serum and Plasma
Endothelin-1 Quantikine ELISA Kit DET100