Flutax 1
Chemical Name: (2aR,4S,4aS,6R,9S,11S,12S,12aR,12bS)-6,12b-Bis(acetyloxy)-9-[(2R,3S)-3-(benzoylamino)-2-hydroxy-1-oxo-3-phenylpropoxy]-12-(benzoyloxy)-2a,3,4,4a,5,6,9,10,11,12,12a,12b-dodecahydro-11-hydroxy-4a,8,13,13-tetramethyl-5-oxo-7,11-methano-1H-cyclodeca[3,4]benz[1,2-b]oxet-4-yl ester N-[(3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)carbonyl]-L-alanine
Purity: ≥95%
Biological Activity
Key information: Flutax 1 is a green-fluorescent taxol derivative; for microtubules labeling in live cells.Used for: direct imaging of the microtubule cytoskeleton.
Application: fluorescent microscopy.
Properties and Photophysical Data: Flutax 1 binds microtubules with high affinity (Ka ~ 107M-1). Absorption, fluorescence and fluorescence decay of Flutax 1 in solution are pH sensitive. Excitation and emission maxima (λ) are 495 nm and 520 nm, respectively.
Scientific Data
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Flutax 1 labeled HeLa cells. Flutax 1 was diluted in HBSS and applied to live HeLa cells at a concentration of 2 μM. HeLa cells were incubated for 1 hour at 37°C. After incubation was complete, Flutax 1 was washed off, and fresh HBSS was applied to the cells. Cells were not fixed because Flutax 1 staining is not retained at all after fixation (photobleach). Staining can be done only on Live cells. Flutax 1 staining in live cells diminishes very rapidly when exposed to light, so the cells should be exposed to light as little as possible to retain good fluorescent signal.
Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Background References
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Molecular recognition of Tax. by microtubules. Kinetics and thermodynamics of binding of fluorescent Tax. derivatives to an exposed site.
Diaz et al.
J.Biol.Chem., 2000;275:26265 -
Fast kinetics of Tax. binding to microtubules.
Diaz et al.
J.Biol.Chem., 2003;278:8407
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Citations for Flutax 1
The citations listed below are publications that use Tocris products. Selected citations for Flutax 1 include:
2 Citations: Showing 1 - 2
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Multifunctional MRI/PET Nanobeacons Derived from the in Situ Self-Assembly of Translational Polymers and Clinical Cargo through Coalescent Intermolecular Forces.
Authors: Kaittanis Et al.
Nano Lett 2015;15:8032
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Environment-responsive nanophores for therapy and treatment monitoring via molecular MRI quenching.
Authors: Kaittanis Et al.
Nat Commun 2014;5:3384
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