GABA-A R alpha 4 C-Terminus Antibody

Catalog #: PPS025 Datasheet / COA / SDS

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PPS025

Detection of GABAAR alpha 4 C-Terminus by Western Blot.

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GABA-A R alpha 4 C-Terminus Antibody Summary

Species Reactivity

Human, Mouse, Rat, Bovine, Canine, Primate, Zebrafish

Specificity

Human, mouse, rat, and bovine ~64 kDa GABA_A R alpha 4 subunit, C-Terminus

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Fusion protein from the cytosolic loop of rat GABA_A R alpha 4 subunit, C-Terminus

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1:1000 dilution

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of GABA_AR alpha 4 C-Terminus by Western Blot. Western blot of rat brain (hippocampal) lysates using approximately 5 - 7 μg of tissue. Blots were incubated overnight at 2 - 8° C with anti-GABA_AR, alpha 4 subunit, C-Terminus diluted 1:1000. The antibody labelled the ~64 kDa alpha 4 subunit of the GABA_AR.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: GABA-A R alpha 4

GABA_A ( gamma -aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels. GABA binding to A-type receptors induces anion-selective ion channel opening. These receptors are the principal fast inhibitory neurotransmitter receptors in the CNS. GABA_A receptors are heteropentamer combinations of seven subunit types; alpha, beta, gamma, δ, epsilon, theta, and π. Three subunits, alpha, beta, and gamma, have at least three separate gene products in mammals, and typical GABA_A receptors have some combination of alpha, beta and gamma subunits. The rat alpha 4 isoform is a 67 kDa, 517 amino acid (aa), 4 transmembrane protein with two terminal extracellular regions. The ligand-binding region is in the N-terminus (aa 14 - 221). The alpha 4 subunit is unusual in that it does not seem to preferentially form channel complexes with the gamma subunit. In hippocampal neurons, alpha 4 subunits apparently form GABA receptors that do not cluster, but exist in diffuse networks in nonsynaptic membrane.

References

Darlison, M.G. et al. (2005) Cell. Mol. Neurobiol. 25:607.
Akabas, M.H. (2004) Int. Rev. Neurobiol. 62:1.
Wisden, W. et al. (1991) FEBS Lett. 289:227.
Bencsits, E. et al. (1999) J. Biol. Chem. 274:19613.
Mangan, P.S. et al. (2005) Mol. Pharmacol. 67:775.

Long Name

gamma-Aminobutyric Acid Type A Receptor, alpha-4 Polypeptide

Entrez Gene IDs

2557 (Human); 14397 (Mouse); 140675 (Rat)

Alternate Names

GABA A R alpha 4; GABA(A) receptor subunit alpha-4; GABAAR alpha 4; GABAARa4; GABRA4; gamma-aminobutyric acid (GABA) A receptor, alpha 4; gamma-aminobutyric acid receptor subunit alpha-4