GDP-Biotin-Fucose

Catalog #: ES202 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
ES202-025

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Product Details

Procedure

Supplemental Products

Reviews

Summary Product Datasheets

GDP-Biotin-Fucose Summary

Key Benefits

Learn more about Fluorescent Glycan Labeling and Detection

Formula	C₃₇H₅₇N₁₀O₂₁P₂S
Molecular Weight	1085.93 Da
Formulation	Lyophilized with Tris, at pH 8.0
Stability & Storage	Store the unopened product at ≤ -20 °C. Use a manual defrost freezer and aviod repeated freeze-thaw cycles. Good for 12 months from date of receipt.

Applications

Biotinylation of free glycans as well as glycoproteins and glycolipids.
Detect specific glycan epitopes on glycoproteins as well as cell surface.

Key Features and Benefits:

Can be introduced to proteins and lipids via various fucosyltransferases.
Can be introduced to live cells for imaging.
Minimum side effect on target molecules.
Convenient and User-friendly.

For Details:

Wu, ZL. et al., (2020) Glycobiology 30:970.

Related reagents

Click Chemistry

Enzymes and Detection Reagents for GDP-Biotin-Fucose, ES202

Data Examples

Table 1. Enzyme selection for Glycan Labeling

Fucosyltransferase	R&D Systems, Catalog #	Substrate	GDP-Biotin-Fucose Tolerance
Fucosyltransferase 1/FUT1		Terminal Gal in H antigen	Not determined
Fucosyltransferase 2/FUT2	7770-GT	Terminal Gal in H antigen	Yes
Fucosyltransferase 3/FUT3	4950-GT	GlcNAc in type 1 glycan chain	Yes
Fucosyltransferase 4/FUT4		GlcNAc in terminal lactosamine	Yes
Fucosyltransferase 5/FUT5	4949-GT	GlcNAc in terminal lactosamine	Yes
Fucosyltransferase 6/FUT6		GlcNAc in terminal lactosamine	Yes
Fucosyltransferase 7/FUT7	6409-GT	GlcNAc in sialylated lactosamine	Yes
Fucosyltransferase 8/FUT8	5768-GT	Core GlcNAc in N-glycans	Yes
Fucosyltransferase 9/FUT9	9347-GT	GlcNAc in non-sialylated lactosamine	Yes
Fucosyltransferase 10/FUT10		Unknown	Unknown
Fucosyltransferase 11/FUT11	5964-GT	Unknown	Unknown
Recombinant Human Protein O-fucosyltransferase 1/POFUT1, CF	7409-GT	Notch receptor	Not determined

Specifications

Storage

Store the unopened product at < -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Good for 12 months from date of receipt.

Species

Multi-Species

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Glycobiology-related Enzymes

Assay Procedure

Sample Protocol for Labeling & Detecting glycan on Fetuin with Biotinylated Fucose

Protocols are guidelines. Parameters need to be optimized by end users.

Materials

Assay Buffer: 25 mM Tris, 10 mM MnCl₂, pH 7.5

Sample glycoprotein

Recombinant Fucosyltransferase

GDP-Biotin-Fucose (R&D Systems, Catalog # ES202)

Protein Sample Loading Dye

SDS-PAGE and Western Blot reagents or equivalent

TBST Buffer: 25 mM Tris, 137 mM NaCl, 0.1% Tween-20, pH 7.5

Commercial ECL (Enhanced Chemiluminescence) Reagents

Nitrocellulose Membrane

10% Fat Free Milk

Streptavidin-HRP (R&D Systems, Catalog # DY998)

Assay Procedure

Prepare reaction mixture by combining a sample glycoprotein (ideally between 1 to 10 µg), 0.25 nmol GDP-Biotin-Fucose, 0.5 μg of a Recombinant Fucosyltransferase, and add Assay Buffer to the final volume to 30 μL.
Prepare a negative control by repeating above but omitting the Fucosyltransferases.
Incubate all the reactions and controls at 37 °C for 60 minutes.
Stop the reactions and controls by adding appropriate volume of Protein Sample Loading Dye to each reaction.
Separate the reactions and controls by SDS-PAGE.
Blot the gel to a nitrocellulose membrane.
Block the blot with 10% fat-free milk for 5 minutes.
Thoroughly wash the membrane with TBST Buffer by changing buffer three times for a total of 30 minutes.
Incubate the blot with 25 ng/mL Streptavidin-HRP in 30 mL TBST buffer for 30 minutes.
Thoroughly wash the membrane with TBST Buffer by changing buffer three times for a total of 45 minutes.
Detect with Commercial ECL (Enhanced Chemiluminescence) Reagents.