GFP Antibody Summary
Ala2-Tyr238
Accession # P42212
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GFP
The green fluorescent protein (GFP), originally discovered in the jellyfish, Aequorea victoria, is composed of 238 amino acids and has a molecular weight of 27 kDa. GFP is in the shape of a cylinder, comprising 11 strands of beta-sheet outside, a single alpha helical strand containing the chromophore running through the center, and short helical segments on the ends. GFP transduces the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. GFP has been widely used as a tracer in various diciplines of biology research, such as fluorescence microscopy, reporter gene expression, and in vivo imaging. Laboratory mutation of GFP generated the widely used analog GFPuv, which has brighter fluorescence and more desirable expression properties.
Product Datasheets
Citation for GFP Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Pseudomonas aeruginosa-induced apoptosis in airway epithelial cells is mediated by gap junctional communication in a JNK-dependent manner.
Authors: Losa D, Kohler T, Bellec J, Dudez T, Crespin S, Bacchetta M, Boulanger P, Hong S, Morel S, Nguyen T, van Delden C, Chanson M
J Immunol, 2014-04-14;192(10):4804-12.
Species: Human
Sample Types: Whole Cells
Applications: IHC
FAQs
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