Glutathione S-Transferase/GST Antibody

Name: Glutathione S-Transferase/GST Antibody
Brand: R&D Systems
SKU: MAB9499
Rating: 4 (1 reviews)

Catalog #: MAB9499 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9499-100
MAB9499-SP

Detection of Glutathione S‑Transferase/GST by Western Blot.

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All Glutathione S-Transferase/GST Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Product Datasheets Related Research Areas

Glutathione S-Transferase/GST Antibody Summary

Specificity

Detects Glutathione S‑Transferase/GST in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 965427

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant Glutathione S‑Transferase/GST epitope tag
Accession # P08515

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Glutathione S‑Transferase/GST by Western Blot. Western blot shows lysates of GST tagged ISG-15, GST tagged Ubiquitin, GST tagged FLAG®, HEK293T human embryonic kidney cell line,E. colitransfected with GST tagged BRDG1-SH2, andSf21 transfected GST tagged PARK1. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Glutathione S-Transferase/GST Monoclonal Antibody (Catalog # MAB9499) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Glutathione S-Transferase/GST as indicated. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.