Goat IgG HRP-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of Goat IgG Primary Antibody by Western Blot Western blot shows detection of Goat Anti-Human/Mouse/Rat Syntaxin 1A Polyclonal Antibody (Catalog # AF7237) using a 1:1000 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human IgG by Simple Western Evaluation of ZL-2201 antiproliferative activity in vitro. A, Chemical structure of ZL-2201, a potent and selective DNA-PK inhibitor. B, Concentration-dependent response to ZL-2201 in M059J and M059K glioblastoma cancer cells measured by CTG after 6-day of treatment. The graph represents the average inhibitory (IC50) values (n = 3). C, Concentration-dependent response to ZL-2201 in CRISPR KO of ATM in A549 and FaDu cancer cells measured by CTG after 6 days of treatment. The graph represents the average inhibitory (IC50) values (n = 2–5). D, IC50 responses to ZL-2201 in ATM mutant cell lines (black bars) versus ATM WT A549 cell line (gray bar). Cell growth was measured by CTG after 6 days of treatment. The graph represents the average inhibitory (IC50) values (n = 2–6). NCI-H1703 (E) and A549 (F) cancer cells were treated with bleomycin (10 μmol/L) for 2 hours followed by the addition of increasing concentration of ZL-2201 for 2 hours. Whole-cell lysates were harvested, and concentration-dependent inhibition of DNA-PK protein were analyzed by Simple Western. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37663435), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 6 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Product Datasheets
Citations for Goat IgG HRP-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
129
Citations: Showing 1 - 10
Filter your results:
Filter by:
-
Infection by the Protozoan Parasite Toxoplasma gondii Inhibits Host MNK1/2-eIF4E Axis to Promote Its Survival
Authors: Louis-Philippe Leroux, Visnu Chaparro, Maritza Jaramillo
Frontiers in Cellular and Infection Microbiology
-
Proteomic Analysis of Urine from California Sea Lions ( Zalophus californianus): A Resource for Urinary Biomarker Discovery
Authors: Benjamin A. Neely, Katherine C. Prager, Alison M. Bland, Christine Fontaine, Frances M. Gulland, Michael G. Janech
Journal of Proteome Research
-
Yersinia pseudotuberculosis YopJ Limits Macrophage Response by Downregulating COX-2-Mediated Biosynthesis of PGE2 in a MAPK/ERK-Dependent Manner
Authors: Austin E. F. Sheppe, John Santelices, Daniel M. Czyz, Mariola J. Edelmann
Microbiology Spectrum
-
Protection of insulin‑like growth factor 1 on experimental peripheral neuropathy in diabetic mice
Authors: Hua Wang, Hao Zhang, Fuming Cao, Jiaping Lu, Jin Tang, Huizhi Li et al.
Molecular Medicine Reports
-
Microglial- and Astrocyte-Specific Expression of Purinergic Signaling Components and Inflammatory Mediators in the Rat Hippocampus During Trimethyltin-Induced Neurodegeneration
Authors: Dragi? M, Mitrovi? N, Ad�i? M et al.
ASN Neuro
-
Efficacious, safe and stable inhibition of corneal neovascularization by AAV vectored anti-VEGF therapeutics
Authors: Su W, Sun S, Tian B et al.
Mol Ther Methods Clin Dev
-
Discovery and Characterization of ZL-2201, a Potent, Highly Selective, and Orally Bioavailable Small-molecule DNA-PK Inhibitor.
Authors: Lal S, Bhola N H et al.
Cancer Res Commun
-
Keratinocyte-derived defensins activate neutrophil-specific receptors Mrgpra2a/b to prevent skin dysbiosis and bacterial infection
Authors: Xintong Dong, Nathachit Limjunyawong, Elizabeth I. Sypek, Gaofeng Wang, Roger V. Ortines, Christine Youn et al.
Immunity
-
Overlooked benefits of using polyclonal antibodies
Authors: Carl A Ascoli, Birte Aggeler
BioTechniques
-
Hereditary protein S deficiency leads to ischemic stroke.
Authors: Wang ZH, Zhao ZJ, Xu K et al.
Mol Med Rep
-
Klebsiella pneumoniae hijacks the Toll-IL-1R protein SARM1 in a type I IFN-dependent manner to antagonize host immunity
Authors: Feriotti C, SA-Pessoa J, CalderOn-GonzAlez R et al.
Cell reports
-
PAPP-A proteolytic activity enhances IGF bioactivity in ascites from women with ovarian carcinoma
Authors: Jacob Thomsen, Rikke Hjortebjerg, Ulrick Espelund, Gitte Ørtoft, Poul Vestergaard, Nils E. Magnusson et al.
Oncotarget
-
Genetically Engineered Pigs as Efficient Salivary Gland Bioreactors for Production of Therapeutically Valuable Human Nerve Growth Factor
Authors: F Zeng, S Liao, Z Kuang, Q Zhu, H Wei, J Shi, E Zheng, Z Xu, S Huang, L Hong, T Gu, J Yang, H Yang, G Cai, S Moisyadi, J Urschitz, Z Li, Z Wu
Cells, 2022-08-02;11(15):.
-
Placental deficiency of the (pro)renin receptor ((P)RR) reduces placental development and functional capacity
Authors: Lachlan G. Schofield, Richard G. S. Kahl, Samantha L. Rodrigues, Joshua J. Fisher, Saije K. Endacott, Sarah J. Delforce et al.
Frontiers in Cell and Developmental Biology
-
Editing the immunopeptidome of melanoma cells using a potent inhibitor of endoplasmic reticulum aminopeptidase 1 (ERAP1)
Authors: Despoina Koumantou, Eilon Barnea, Adrian Martin-Esteban, Zachary Maben, Athanasios Papakyriakou, Anastasia Mpakali et al.
Cancer Immunology, Immunotherapy
-
Cytokine-Laden Extracellular Vesicles Predict Patient Prognosis after Cerebrovascular Accident
Authors: Anthony Fringuello, Philip D. Tatman, Tadeusz Wroblewski, John A. Thompson, Xiaoli Yu, Kevin O. Lillehei et al.
International Journal of Molecular Sciences
-
Caspase-8 inhibition improves the outcome of bacterial infections in mice by promoting neutrophil activation
Authors: Germana Lentini, Agata Famà, Giuseppe Valerio De Gaetano, Francesco Coppolino, Ahlem Khachroub Mahjoub, Liv Ryan et al.
Cell Reports Medicine
-
Ubiquitin is a versatile scaffold protein for the generation of molecules with de novo binding and advantageous drug-like properties
Authors: Florian Job, Florian Settele, Susan Lorey, Chris Rundfeldt, Lars Baumann, Annette G. Beck-Sickinger et al.
FEBS Open Bio
-
Magnesium isoglycyrrhizinate ameliorates high fructose-induced liver fibrosis in rat by increasing miR-375-3p to suppress JAK2/STAT3 pathway and TGF-beta 1/Smad signaling
Authors: Yan-zi Yang, Xiao-juan Zhao, Hong-jiang Xu, Shan-chun Wang, Ying Pan, Shui-juan Wang et al.
Acta Pharmacologica Sinica
-
Apoptosis-inducing effect of myxoma virus on human neuroglioma cell lines
Authors: Qiu-Sheng Zhang, Meng Zhang, Xian-Jian Huang, Xiao-Jia Liu, Wei-Ping Li
Experimental and Therapeutic Medicine
-
Enhancing viability and angiogenic efficacy of mesenchymal stem cells via HSP90 alpha and HSP27 regulation based on ROS stimulation for wound healing
Authors: Inwoo Seo, Sung‐Won Kim, Jiyu Hyun, Yu‐Jin Kim, Hyun Su Park, Jeong‐Kee Yoon et al.
Bioengineering & Translational Medicine
-
Effect of Fluticasone Furoate on Interleukin 6 Secretion From Adenoid Tissues in Children With Obstructive Sleep Apnea
Authors: Rania Esteitie, Janaki Emani, Shilpy Sharma, Dana L. Suskind, Fuad M. Baroody
Archives of Otolaryngology–Head & Neck Surgery
-
Intermittent Theta Burst Stimulation Improves Motor and Behavioral Dysfunction through Modulation of NMDA Receptor Subunit Composition in Experimental Model of Parkinson’s Disease
Authors: Milica Zeljkovic Jovanovic, Jelena Stanojevic, Ivana Stevanovic, Andjela Stekic, Samuel J. Bolland, Nebojsa Jasnic et al.
Cells
-
Roles of Gremlin 1 and Gremlin 2 in regulating ovarian primordial to primary follicle transition
Authors: Eric E. Nilsson, Ginger Larsen, Michael K. Skinner
REPRODUCTION
-
Expression of Ectonucleoside Triphosphate Diphosphohydrolase 2 (NTPDase2) Is Negatively Regulated Under Neuroinflammatory Conditions In Vivo and In Vitro
Authors: Milorad Dragic, Katarina Mihajlovic, Marija Adzic, Marija Jakovljevic, Marina Zaric Kontic, Nataša Mitrović et al.
ASN Neuro
-
Fortifying angiogenic efficacy of conditioned media using phototoxic‐free blue light for wound healing
Authors: Sung‐Won Kim, Gwang‐Bum Im, Yeong Hwan Kim, Suk Ho Bhang
Bioengineering & Translational Medicine
-
A Novel Triple-Cell Two-Dimensional Model to Study Immune-Vascular Interplay in Atherosclerosis
Authors: Jonathan Noonan, Gianluca Grassia, Neil MacRitchie, Paul Garside, Tomasz J. Guzik, Angela C. Bradshaw et al.
Frontiers in Immunology
-
Oncolytic virotherapy with human telomerase reverse transcriptase promoter regulation enhances cytotoxic effects against gastric cancer
Authors: T Kato, M Nakamori, S Matsumura, M Nakamura, T Ojima, H Fukuhara, Y Ino, T Todo, H Yamaue
Oncology Letters, 2021-04-23;21(6):490.
-
SARS-CoV-2 Employ BSG/CD147 and ACE2 Receptors to Directly Infect Human Induced Pluripotent Stem Cell-Derived Kidney Podocytes
Authors: Titilola D. Kalejaiye, Rohan Bhattacharya, Morgan A. Burt, Tatianna Travieso, Arinze E. Okafor, Xingrui Mou et al.
Frontiers in Cell and Developmental Biology
-
Interferon-gamma and tumor necrosis factor-alpha act synergistically to up-regulate tissue factor in alveolar epithelial cells
Authors: Julie A. Bastarache, Sara C. Sebag, Brandon S. Grove, Lorraine B. Ware
Experimental Lung Research
-
Subtilase cytotoxin from Shiga-toxigenic Escherichia coli impairs the inflammasome and exacerbates enteropathogenic bacterial infection
Authors: Hiroyasu Tsutsuki, Tianli Zhang, Kinnosuke Yahiro, Katsuhiko Ono, Yukio Fujiwara, Sunao Iyoda et al.
iScience
-
Anatomic position determines oncogenic specificity in melanoma
Authors: Joshua M. Weiss, Miranda V. Hunter, Nelly M. Cruz, Arianna Baggiolini, Mohita Tagore, Yilun Ma et al.
Nature
-
Combination Therapy of Novel Oncolytic Adenovirus with Anti-PD1 Resulted in Enhanced Anti-Cancer Effect in Syngeneic Immunocompetent Melanoma Mouse Model
Authors: Garofalo M, Bertinato L, Staniszewska M et al.
Pharmaceutics
-
Impact of heat therapy on recovery after eccentric exercise in humans
Authors: Kyoungrae Kim, Shihuan Kuang, Qifan Song, Timothy P. Gavin, Bruno T. Roseguini
Journal of Applied Physiology
-
The use of an in vitro 3D melanoma model to predict in vivo plasmid transfection using electroporation
Authors: Bernadette Marrero, Richard Heller
Biomaterials
-
OmpA Protein-Deficient Acinetobacter baumannii Outer Membrane Vesicles Trigger Reduced Inflammatory Response
Authors: Jūratė Skerniškytė, Emilija Karazijaitė, Asta Lučiūnaitė, Edita Sužiedėlienė
Pathogens
-
USP8 inhibition regulates autophagy flux and controls Salmonella infection
Authors: Santelices J, Ou M, Maegawa GHB et al.
Frontiers in Cellular and Infection Microbiology
-
Selection and Validation of siRNAs Preventing Uptake and Replication of SARS-CoV-2
Authors: Maik Friedrich, Gabriele Pfeifer, Stefanie Binder, Achim Aigner, Philippe Vollmer Barbosa, Gustavo R. Makert et al.
Frontiers in Bioengineering and Biotechnology
-
SerpinA3N deficiency attenuates steatosis and enhances insulin signaling in male mice
Authors: Melanie Tran, Md Golam Mostofa, Michael Picard, Jianguo Wu, Li Wang, Dong-Ju Shin
Journal of Endocrinology
-
Ceramide-induced cleavage of GPR64 intracellular domain drives Ewing sarcoma
Authors: Suvarna, K;Jayabal, P;Ma, X;Wang, H;Chen, Y;Weintraub, ST;Han, X;Houghton, PJ;Shiio, Y;
Cell reports
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The Elevated Inflammatory Status of Neutrophils Is Related to In-Hospital Complications in Patients with Acute Coronary Syndrome and Has Important Prognosis Value for Diabetic Patients
Authors: Barbu, E;Mihaila, AC;Gan, AM;Ciortan, L;Macarie, RD;Tucureanu, MM;Filippi, A;Stoenescu, AI;Petrea, SV;Simionescu, M;Balanescu, SM;Butoi, E;
International journal of molecular sciences
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Vascular architecture regulates mesenchymal stromal cell heterogeneity via P53-PDGF signaling in the mouse incisor
Authors: Guo, T;Pei, F;Zhang, M;Yamada, T;Feng, J;Jing, J;Ho, TV;Chai, Y;
Cell stem cell
Species: Transgenic Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
N-cadherin directs the collective Schwann cell migration required for nerve regeneration through Slit2/3-mediated contact inhibition of locomotion
Authors: Hoving, JJA;Harford-Wright, E;Wingfield-Digby, P;Cattin, AL;Campana, M;Power, A;Morgan, T;Torchiaro, E;Quereda, V;Lloyd, AC;
eLife
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Proteomic Assessment of SKBR3/HER2+ Breast Cancer Cellular Response to Lapatinib and Investigational Ipatasertib Kinase Inhibitors
Authors: Karcini, A;Mercier, NR;Lazar, IM;
bioRxiv : the preprint server for biology
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Long noncoding RNA Malat1 protects against osteoporosis and bone metastasis
Authors: Zhao, Y;Ning, J;Teng, H;Deng, Y;Sheldon, M;Shi, L;Martinez, C;Zhang, J;Tian, A;Sun, Y;Nakagawa, S;Yao, F;Wang, H;Ma, L;
Nature communications
Species: Mouse
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
The IRAK1/IRF5 axis initiates IL-12 response by dendritic cells and control of Toxoplasma gondii infection
Authors: Pereira, M;Ramalho, T;Andrade, WA;Durso, DF;Souza, MC;Fitzgerald, KA;Golenbock, DT;Silverman, N;Gazzinelli, RT;
Cell reports
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Type 3 secretion system induced leukotriene B4 synthesis by leukocytes is actively inhibited by Yersinia pestis to evade early immune recognition
Authors: Brady, A;Sheneman, KR;Pulsifer, AR;Price, SL;Garrison, TM;Maddipati, KR;Bodduluri, SR;Pan, J;Boyd, NL;Zheng, JJ;Rai, SN;Hellmann, J;Haribabu, B;Uriarte, SM;Lawrenz, MB;
PLoS pathogens
Species: Bacteria
Sample Types: Cell Lysates
Applications: Western Blot -
Urine-derived bladder cancer organoids (urinoids) as a tool for cancer longitudinal response monitoring and therapy adaptation
Authors: Viergever, BJ;Raats, DAE;Geurts, V;Mullenders, J;Jonges, TN;van der Heijden, MS;van Es, JH;Kranenburg, O;Meijer, RP;
British journal of cancer
Species: Human
Sample Types: Organoids
Applications: IHC -
Expression of polymeric immunoglobulin receptor (PIGR) and the effect of PIGR overexpression on breast cancer cells
Authors: Asanprakit, W;Lobo, DN;Eremin, O;Bennett, AJ;
Scientific reports
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Impaired Plakophilin-2 in obesity breaks cell cycle dynamics to breed adipocyte senescence
Authors: Lluch, A;Latorre, J;Serena-Maione, A;Espadas, I;Caballano-Infantes, E;Moreno-Navarrete, JM;Oliveras-Cañellas, N;Ricart, W;Malagón, MM;Martin-Montalvo, A;Birchmeier, W;Szymanski, W;Graumann, J;Gómez-Serrano, M;Sommariva, E;Fernández-Real, JM;Ortega, FJ;
Nature communications
Species: Human
Sample Types: Cell Culture Supernates
Applications: Secondary Antibody -
RNF4 and USP7 cooperate in ubiquitin-regulated steps of DNA replication
Authors: Chang, YC;Lin, K;Baxley, RM;Durrett, W;Wang, L;Stojkova, O;Billmann, M;Ward, H;Myers, CL;Bielinsky, AK;
Open biology
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Toxoplasma gondii inhibits the expression of autophagy-related genes through AKT-dependent inactivation of the transcription factor FOXO3a
Authors: Diez, AF;Leroux, LP;Chagneau, S;Plouffe, A;Gold, M;Chaparro, V;Jaramillo, M;
mBio
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
In vivo evolution of a Klebsiella pneumoniae capsule defect promotes complement-mediated opsono-phagocytosis and persistence during recurrent infection
Authors: Bain, W;Ahn, B;Penaloza, H;McElheny, C;Tolman, N;van der Geest, R;Gonzalez-Ferrer, S;Chen, N;An, X;Hosuru, R;Tabary, M;Papke, E;Kohli, N;Farooq, N;Bachman, W;Olonisakin, T;Xiong, Z;Griffith, MP;Sullivan, M;Franks, J;Mustapha, M;Iovleva, A;Suber, T;Shanks, RM;Ferreira, V;Stolz, DB;Van Tyne, D;Doi, Y;Lee, J;
bioRxiv : the preprint server for biology
Species: Bacteria
Sample Types: Whole Cells
Applications: ELISA Detection -
Distinct modulation of cellular immunopeptidome by the allosteric regulatory site of ER aminopeptidase 1
Authors: Temponeras, I;Samiotaki, M;Koumantou, D;Nikopaschou, M;Kuiper, JJW;Panayotou, G;Stratikos, E;
European journal of immunology
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
USP8 inhibition regulates autophagy flux and controls Salmonella infection
Authors: Santelices J, Ou M, Maegawa GHB et al.
Frontiers in Cellular and Infection Microbiology
-
Survival-based CRISPR genetic screens across a panel of permissive cell lines identify common and cell-specific SARS-CoV-2 host factors
Authors: K Chan, AG Farias, H Lee, F Guvenc, P Mero, KR Brown, H Ward, M Billmann, K Aulakh, A Astori, S Haider, E Marcon, U Braunschwe, S Pu, A Habsid, AH Yan Tong, N Christie-H, P Budylowski, A Ghalami, S Mubareka, F Maguire, A Banerjee, KL Mossman, J Greenblatt, SD Gray-Owen, B Raught, BJ Blencowe, M Taipale, C Myers, J Moffat
Heliyon, 2022-12-30;0(0):e12744.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Targeting PCSK9 in Liver Cancer Cells Triggers Metabolic Exhaustion and Cell Death by Ferroptosis
Authors: M Alannan, H Fatrouni, V Trézéguet, F Dittrich-D, P Moreau, G Siegfried, B Liet, AM Khatib, CF Grosset, B Badran, H Fayyad-Kaz, AJ Merched
Cells, 2022-12-23;12(1):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
M1 macrophages evoke an increase in polymeric immunoglobulin receptor (PIGR) expression in MDA-MB468 breast cancer cells through secretion of interleukin-1beta
Authors: W Asanprakit, DN Lobo, O Eremin, AJ Bennett
Scientific Reports, 2022-10-07;12(1):16842.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Inferring and perturbing cell fate regulomes in human brain organoids
Authors: JS Fleck, SMJ Jansen, D Wollny, F Zenk, M Seimiya, A Jain, R Okamoto, M Santel, Z He, JG Camp, B Treutlein
Nature, 2022-10-05;0(0):.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
The IRAK4 scaffold integrates TLR4-driven TRIF and MYD88 signaling pathways
Authors: M Pereira, DF Durso, CE Bryant, EA Kurt-Jones, N Silverman, DT Golenbock, RT Gazzinelli
Cell Reports, 2022-08-16;40(7):111225.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Seminal-Plasma-Mediated Effects on Sperm Performance in Humans
Authors: T Turunen, M Magris, M Malinen, J Kekäläinen
Cells, 2022-07-08;11(14):.
Species: Human
Sample Types: Seminal Plasma
Applications: Western Blot -
Persistent Infection of a Canine Histiocytic Sarcoma Cell Line with Attenuated Canine Distemper Virus Expressing Vasostatin or Granulocyte-Macrophage Colony-Stimulating Factor
Authors: K Marek, F Armando, VM Nippold, K Rohn, P Plattet, G Brogden, G Gerold, W Baumgärtne, C Puff
International Journal of Molecular Sciences, 2022-05-31;23(11):.
Species: Canine
Sample Types: Cell Lysates
Applications: Western Blot -
Head-to-Head Study of Developmental Neurotoxicity and Resultant Phenotype in Rats: alpha-Hexabromocyclododecane versus Valproic Acid, a Recognized Model of Reference for Autism Spectrum Disorders
Authors: C Morel, A Christophe, K Maguin-Gat, J Paoli, JD Turner, H Schroeder, N Grova
Toxics, 2022-04-06;10(4):.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Piceatannol suppresses inflammation and promotes apoptosis in rheumatoid arthritis?fibroblast?like synoviocytes by inhibiting the NF?kappaB and MAPK signaling pathways
Authors: X Gao, X Kang, H Lu, E Xue, R Chen, J Pan, J Ma
Molecular Medicine Reports, 2022-03-24;25(5):.
Species: Rat
Sample Types: Whole Cells
Applications: IF -
Glioblastoma mutations alter EGFR dimer structure to prevent ligand bias
Authors: C Hu, CA Leche, A Kiyatkin, Z Yu, SE Stayrook, KM Ferguson, MA Lemmon
Nature, 2022-02-09;602(7897):518-522.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
ERAP2 Inhibition Induces Cell-Surface Presentation by MOLT-4 Leukemia Cancer Cells of Many Novel and Potentially Antigenic Peptides
Authors: I Temponeras, G Stamatakis, M Samiotaki, D Georgiadis, H Pratsinis, G Panayotou, E Stratikos
International Journal of Molecular Sciences, 2022-02-08;23(3):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
TREM-1 is required for enhanced OpZ-induced superoxide generation following priming
Authors: S Murthy, S Baruah, JL Bowen, K Keck, BA Wagner, GR Buettner, DB Sykes, J Klesney-Ta
Journal of leukocyte biology, 2022-01-24;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
A targetable LIFR-NF-kappaB-LCN2 axis controls liver tumorigenesis and vulnerability to ferroptosis
Authors: F Yao, Y Deng, Y Zhao, Y Mei, Y Zhang, X Liu, C Martinez, X Su, RR Rosato, H Teng, Q Hang, S Yap, D Chen, Y Wang, MM Chen, M Zhang, H Liang, D Xie, X Chen, H Zhu, JC Chang, MJ You, Y Sun, B Gan, L Ma
Nature Communications, 2021-12-17;12(1):7333.
Species: Human, Mouse
Sample Types: Whole Tissue
Applications: IHC -
Glucocorticoid receptor regulates PD-L1 and MHC-I in pancreatic cancer cells to promote immune evasion and immunotherapy resistance
Authors: Y Deng, X Xia, Y Zhao, Z Zhao, C Martinez, W Yin, J Yao, Q Hang, W Wu, J Zhang, Y Yu, W Xia, F Yao, D Zhao, Y Sun, H Ying, MC Hung, L Ma
Nature Communications, 2021-12-06;12(1):7041.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Tumour DDR1 promotes collagen fibre alignment to instigate immune exclusion
Authors: X Sun, B Wu, HC Chiang, H Deng, X Zhang, W Xiong, J Liu, AM Rozeboom, BT Harris, E Blommaert, A Gomez, RE Garcia, Y Zhou, P Mitra, M Prevost, D Zhang, D Banik, C Isaacs, D Berry, C Lai, K Chaldekas, PS Latham, CA Brantner, A Popratilof, VX Jin, N Zhang, Y Hu, MA Pujana, TJ Curiel, Z An, R Li
Nature, 2021-11-03;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Microglial- and Astrocyte-Specific Expression of Purinergic Signaling Components and Inflammatory Mediators in the Rat Hippocampus During Trimethyltin-Induced Neurodegeneration
Authors: Dragi? M, Mitrovi? N, Ad�i? M et al.
ASN Neuro
-
Thresholds of Endoglin Expression in Endothelial Cells Explains Vascular Etiology in Hereditary Hemorrhagic Telangiectasia Type 1
Authors: G Galaris, K Montagne, JH Thalgott, GJPE Goujon, S van den Dr, S Martin, HJ Mager, CL Mummery, TJ Rabelink, F Lebrin
International Journal of Molecular Sciences, 2021-08-19;22(16):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Morus alba Root Extract Induces the Anagen Phase in the Human Hair Follicle Dermal Papilla Cells
Authors: J Hyun, J Im, SW Kim, HY Kim, I Seo, SH Bhang
Pharmaceutics, 2021-07-27;13(8):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
IL-6/STAT3 signaling activation exacerbates high fructose-induced podocyte hypertrophy by ketohexokinase-A-mediated tristetraprolin down-regulation
Authors: J Zhou, J Yang, YM Wang, H Ding, TS Li, ZH Liu, L Chen, RQ Jiao, DM Zhang, LD Kong
Cellular Signalling, 2021-07-10;86(0):110082.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Immunomodulatory mAbs as Tools to Investigate on Cis-Interaction of PD-1/PD-L1 on Tumor Cells and to Set Up Methods for Early Screening of Safe and Potent Combinatorial Treatments
Authors: C Vetrei, M Passariell, G Froechlich, R Rapuano Le, N Zambrano, C De Lorenzo
Cancers, 2021-06-08;13(12):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Mechanisms of angiogenic incompetence in Hutchinson-Gilford progeria via downregulation of endothelial NOS
Authors: YG Gete, LW Koblan, X Mao, M Trappio, B Mahadik, JP Fisher, DR Liu, K Cao
Aging Cell, 2021-06-04;0(0):e13388.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Vaccine delivery alerts innate immune systems for more immunogenic vaccination
Authors: Z Li, Y Cao, Y Li, Y Zhao, X Chen
JCI Insight, 2021-04-08;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Glycan Epitopes on 201B7 Human-Induced Pluripotent Stem Cells Using R-10G and R-17F Marker Antibodies
Authors: Y Nagai, H Nakao, A Kojima, Y Komatsubar, Y Ohta, N Kawasaki, N Kawasaki, H Toyoda, T Kawasaki
Biomolecules, 2021-03-29;11(4):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
LncRNA H19-Derived miR-675-5p Accelerates the Invasion of Extravillous Trophoblast Cells by Inhibiting GATA2 and Subsequently Activating Matrix Metalloproteinases
Authors: M Ogoyama, A Ohkuchi, H Takahashi, D Zhao, S Matsubara, T Takizawa
International Journal of Molecular Sciences, 2021-01-27;22(3):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Development of an engineered peptide antagonist against periostin to overcome doxorubicin resistance in breast cancer
Authors: KK Oo, T Kamolhan, A Soni, S Thongchot, C Mitrpant, P O-Charoenr, C Thuwajit, P Thuwajit
BMC Cancer, 2021-01-14;21(1):65.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Cornus officinalis var. koreana Kitam polyphenol extract decreases pro-inflammatory markers in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages by reducing Akt phosphorylation
Authors: RS Najjar, NS Akhavan, S Pourafshar, BH Arjmandi, RG Feresin
Journal of ethnopharmacology, 2020-12-24;0(0):113734.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
High level of interleukin-33 in cancer cells and cancer-associated fibroblasts correlates with good prognosis and suppressed migration in cholangiocarcinoma
Authors: S Yangngam, S Thongchot, A Pongpaibul, K Vaeteewoot, S Pinlaor, P Thuwajit, S Okada, MA Hermoso, C Thuwajit
J Cancer, 2020-09-23;11(22):6571-6581.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Prion protein signaling induces M2 macrophage polarization and protects from lethal influenza infection in mice
Authors: J Chida, H Hara, K Uchiyama, E Takahashi, H Miyata, H Kosako, Y Tomioka, T Ito, H Horiuchi, H Matsuda, H Kido, S Sakaguchi
PLoS Pathog., 2020-08-26;16(8):e1008823.
Species: Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: Flow Cytometry, Western Blot -
TGF-beta2 interfering oligonucleotides used as adjuvants for microbial vaccines
Authors: L Tu, X Sun, L Yang, T Zhang, X Zhang, X Li, B Dong, Y Liu, M Yang, L Wang, Y Yu
J. Leukoc. Biol., 2020-08-13;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Clinical relevance of B7H3 expression in retinoblastoma
Authors: B Ganesan, S Parameswar, A Sharma, S Krishnakum
Sci Rep, 2020-06-23;10(1):10185.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
Epidermal growth factor regulates the development of stem and progenitor Leydig cells in rats
Authors: X Li, Y Wang, Q Zhu, K Yuan, Z Su, F Ge, RS Ge, Y Huang
J. Cell. Mol. Med., 2020-05-22;0(0):.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
Anti?glycolipid disorder effect of epigallocatechin?3?gallate on high?fat diet and STZ?induced T2DM in mice
Authors: Z Ren, Z Yang, Y Lu, R Zhang, H Yang
Mol Med Rep, 2020-03-26;21(6):2475-2483.
Species: Mouse
Sample Types: Protein
Applications: Western Blot -
Sperm SPACA6 protein is required for mammalian Sperm-Egg Adhesion/Fusion
Authors: S Barbaux, C Ialy-Radio, M Chalbi, E Dybal, M Homps-Legr, M Do Cruzeir, D Vaiman, JP Wolf, A Ziyyat
Sci Rep, 2020-03-24;10(1):5335.
Species: Mouse
Sample Types: Cell Culture Lysates
Applications: Western Blot -
Netazepide Inhibits Expression of Pappalysin 2 in Type-1 Gastric Neuroendocrine Tumors
Authors: KA Lloyd, BN Parsons, MD Burkitt, AR Moore, S Papoutsopo, M Boyce, CA Duckworth, K Exarchou, N Howes, L Rainbow, Y Fang, C Oxvig, S Dodd, A Varro, N Hall, DM Pritchard
Cell Mol Gastroenterol Hepatol, 2020-01-28;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Manganese Uptake by A549 Cells is Mediated by Both ZIP8 and ZIP14
Authors: IF Scheiber, NO Alarcon, N Zhao
Nutrients, 2019-06-28;11(7):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Human metapneumovirus activates NOD-like receptor protein 3 inflammasome via its small hydrophobic protein which plays a detrimental role during infection in mice
Authors: VB Lê, J Dubois, C Couture, MH Cavanagh, O Uyar, A Pizzorno, M Rosa-Calat, MÈ Hamelin, G Boivin
PLoS Pathog., 2019-04-09;15(4):e1007689.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Serum Apolipoprotein E and Other Inflammatory Markers Can Identify Non-Responding Patients to a Dendritic Cell Vaccine
Authors: H Leeman, E Kaminska, D Green, M Bodman-Smi, A Gravett, K Bodman-Smi, J Copier, G Coulton, A Fusi, AG Dalgleish
Transl Oncol, 2018-12-08;12(3):397-403.
Species: Human
Sample Types: Serum
Applications: ELISA Development -
Blocking the proteolytic activity of zymogen matriptase�with antibody-based inhibitors
Authors: T Tamberg, Z Hong, D Schepper, S Skovbjerg, DM Dupont, L Vitved, CR Schar, K Skjoedt, LK Vogel, JK Jensen
J. Biol. Chem., 2018-11-08;0(0):.
Applications: Western Blot -
Streptozotocin?induced diabetic mice exhibit reduced experimental choroidal neovascularization but not corneal neovascularization
Authors: G Liu, L Chen, Q Cai, H Wu, Z Chen, X Zhang, P Lu
Mol Med Rep, 2018-09-03;0(0):.
Species: Mouse
Sample Types: Whole Tissue
-
Pentosan polysulfate inhibits IL-1?-induced iNOS, c-Jun and HIF-1? upregulation in canine articular chondrocytes
Authors: EC Bwalya, S Kim, J Fang, HMS Wijekoon, K Hosoya, M Okumura
PLoS ONE, 2017-05-04;12(5):e0177144.
Species: Canine
Sample Types: Whole Cells
Applications: Western Blot -
Mitochondrial CaMKII inhibition in airway epithelium protects against allergic asthma
Authors: SC Sebag, OM Koval, JD Paschke, CJ Winters, OA Jaffer, R Dworski, FS Sutterwala, ME Anderson, IM Grumbach
JCI Insight, 2017-02-09;2(3):e88297.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Effect of 20-Hydroxyecdysone on Proteolytic Regulation in Skeletal Muscle Atrophy
Authors: Apichart Suksamrarn
In Vivo, 2016-11-12;30(6):869-877.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Reversible linkage of two distinct small molecule inhibitors of Myc generates a dimeric inhibitor with improved potency that is active in myc over-expressing cancer cell lines.
Authors: Wanner, Jutta, Romashko, Darlene, Werner, Douglas, May, Earl W, Peng, Yue, Schulz, Ryan, Foreman, Kenneth, Russo, Suzanne, Arnold, Lee D, Pingle, Maneesh, Bergstrom, Donald E, Barany, Francis, Thomson, Stuart
PLoS ONE, 2015-04-15;10(4):e0121793.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Identification of the avian RBP7 gene as a new adipose-specific gene and RBP7 promoter-driven GFP expression in adipose tissue of transgenic quail.
Authors: Ahn J, Shin S, Suh Y, Park J, Hwang S, Lee K
PLoS ONE, 2015-04-13;10(4):e0124768.
Species: Avian - Quail, Avian - Turkey, Chicken
Sample Types: Tissue Homogenates
Applications: Western Blot -
Identification and characterization of PERK activators by phenotypic screening and their effects on NRF2 activation.
Authors: Xie W, Pariollaud M, Wixted W, Chitnis N, Fornwald J, Truong M, Pao C, Liu Y, Ames R, Callahan J, Solari R, Sanchez Y, Diehl A, Li H
PLoS ONE, 2015-03-17;10(3):e0119738.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Expression of SOX9 and CDX2 in nongoblet columnar-lined esophagus predicts the detection of Barrett's esophagus during follow-up.
Authors: Zhang X, Westerhoff M, Hart J
Mod Pathol, 2014-11-21;28(5):654-61.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Functional evaluation of the role of C-type lectin domain family 16A at the chromosome 16p13 locus.
Authors: Zouk H, D'Hennezel E, Du X, Ounissi-Benkalha H, Piccirillo C, Polychronakos C
Clin Exp Immunol, 2014-03-01;175(3):485-97.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Mechanical stretch inhibits lipopolysaccharide-induced keratinocyte-derived chemokine and tissue factor expression while increasing procoagulant activity in murine lung epithelial cells.
Authors: Sebag S, Bastarache J, Ware L
J Biol Chem, 2013-01-29;288(11):7875-84.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Lumican, an extracellular matrix proteoglycan, is a novel requisite for hepatic fibrosis.
Authors: Krishnan A, Li X, Kao W, Viker K, Butters K, Masuoka H, Knudsen B, Gores G, Charlton M
Lab Invest, 2012-09-24;92(12):1712-25.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Possible role of Efnb1 protein, a ligand of Eph receptor tyrosine kinases, in modulating blood pressure.
Authors: Wu Z, Luo H, Thorin E, Tremblay J, Peng J, Lavoie JL, Wang Y, Qi S, Wu T, Wu J
J. Biol. Chem., 2012-03-05;287(19):15557-69.
Applications: Western Blot -
Critical role for macrophage migration inhibitory factor (MIF) in Ross River virus-induced arthritis and myositis.
Authors: Herrero LJ, Nelson M, Srikiatkhachorn A, Gu R, Anantapreecha S, Fingerle-Rowson G, Bucala R, Morand E, Santos LL, Mahalingam S
Proc. Natl. Acad. Sci. U.S.A., 2011-07-05;108(29):12048-53.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Serglycin is a major proteoglycan in polarized human endothelial cells and is implicated in the secretion of the chemokine GROalpha/CXCL1.
Authors: Meen AJ, Oynebraten I, Reine TM, Duelli A, Svennevig K, Pejler G, Jenssen T, Kolset SO
J. Biol. Chem., 2010-11-12;286(4):2636-47.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Anti-inflammatory activity of nanocrystalline silver-derived solutions in porcine contact dermatitis.
Authors: Nadworny PL, Wang J, Tredget EE
J Inflamm (Lond), 2010-02-19;7(0):13.
Species: Porcine
Sample Types: Whole Tissue
Applications: IHC-P -
Identification of macrophage inhibitory cytokine-1 in adipose tissue and its secretion as an adipokine by human adipocytes.
Authors: Ding Q, Mracek T, Gonzalez-Muniesa P, Kos K, Wilding J, Trayhurn P, Bing C
Endocrinology, 2008-12-12;150(4):1688-96.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Production of C5a by ASP, a serine protease released from Aeromonas sobria.
Authors: Nitta H, Imamura T, Wada Y, Irie A, Kobayashi H, Okamoto K, Baba H
J. Immunol., 2008-09-01;181(5):3602-8.
Species: Human
Sample Types: Plasma
Applications: Western Blot -
ST2 is essential for Th2 responsiveness and resistance to pseudomonas aeruginosa keratitis.
Authors: Huang X, Du W, Barrett RP, Hazlett LD
Invest. Ophthalmol. Vis. Sci., 2007-10-01;48(10):4626-33.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Serum amyloid alpha 1-2 are not required for liver inflammation in the 4T1 murine breast cancer model
Authors: Chenfeng He, Riyo Konishi, Ayano Harata, Yuki Nakamura, Rin Mizuno, Mayuko Yoda et al.
Frontiers in Immunology
-
The exosomal integrin alpha5beta1/AEP complex derived from epithelial ovarian cancer cells promotes peritoneal metastasis through regulating mesothelial cell proliferation and migration
Authors: Li X, Tang M, Zhu Q et al.
Cell Oncol (Dordr)
-
Gene Regulatory Network Inference of Immunoresponsive Gene 1 (IRG1) Identifies Interferon Regulatory Factor 1 (IRF1) as Its Transcriptional Regulator in Mammalian Macrophages
Authors: Aravind Tallam, Thaneer M. Perumal, Paul M. Antony, Christian Jäger, Joëlle V. Fritz, Laurent Vallar et al.
PLOS ONE
-
Genetic Screens Identify Host Factors for SARS-CoV-2 and Common Cold Coronaviruses
Authors: Wang R, Simoneau CR, Kulsuptrakul J et al.
Cell
-
Non-canonical inflammasome activation analysis in a mouse model of Citrobacter rodentium infection
Authors: Hiroyasu Tsutsuki, Tianli Zhang, Kinnosuke Yahiro, Touya Toyomoto, Tomohiro Sawa
STAR Protocols
-
Enhancing therapeutic efficacy of human adipose-derived stem cells by modulating photoreceptor expression for advanced wound healing
Authors: Sang Ho Lee, Yu-Jin Kim, Yeong Hwan Kim, Han Young Kim, Suk Ho Bhang
Stem Cell Research & Therapy
-
Protein-based identification of quantitative trait loci associated with malignant transformation in two HER2+ cellular models of breast cancer
Authors: Yogesh M Kulkarni, David J Klinke
Proteome Science
-
Loading history changes the morphology and compressive force-induced expression of receptor activator of nuclear factor kappa B ligand/osteoprotegerin in MLO-Y4 osteocytes
Authors: Ziyi Wang, Yao Weng, Yoshihito Ishihara, Naoya Odagaki, Ei Ei Hsu Hlaing, Takashi Izawa et al.
PeerJ
-
Attenuation of Myocardial Injury by HMGB1 Blockade during Ischemia/Reperfusion Is Toll-Like Receptor 2-Dependent
Authors: Jan Mersmann, Franziska Iskandar, Kathrina Latsch, Katharina Habeck, Vera Sprunck, René Zimmermann et al.
Mediators of Inflammation
-
Deorphanizing Caspase-3 and Caspase-9 Substrates In and Out of Apoptosis with Deep Substrate Profiling
Authors: Luam E. Araya, Ishankumar V. Soni, Jeanne A. Hardy, Olivier Julien
ACS Chemical Biology
-
Tissue Levels of CD80, CD163 and CD206 and Their Ratios in Periodontal and Peri-Implant Health and Disease
Authors: Mustafa Yilmaz, Esra Demir, Yigit Firatli, Erhan Firatli, Ulvi Kahraman Gürsoy
Current Issues in Molecular Biology
-
Humanized Biomimetic Nanovesicles for Neuron Targeting
Authors: Assaf Zinger, Caroline Cvetkovic, Manuela Sushnitha, Tomoyuki Naoi, Gherardo Baudo, Morgan Anderson et al.
Advanced Science
-
Oncolytic adenovirus-mediated mda-7/IL-24 expression suppresses osteosarcoma growth and enhances sensitivity to doxorubicin
Authors: ZONGMING LIU, LIBO XU, HONGPING YUAN, YANG ZHANG, XIAONA ZHANG, DONGXU ZHAO
Molecular Medicine Reports
-
A mouse model of cone photoreceptor function loss (cpfl9) with degeneration due to a mutation in Gucy2e
Authors: Anna S. E. N. Naggert, Gayle B. Collin, Jieping Wang, Mark P. Krebs, Bo Chang
Frontiers in Molecular Neuroscience
-
Incorporation of a TGF-beta 2-inhibiting oligodeoxynucleotide molecular adjuvant into a tumor cell lysate vaccine to enhance antiglioma immunity in mice
Authors: Liqun Tu, Zhe Wang, Lei Yang, Xiaomeng Sun, Yunpeng Yao, Peng Zhang et al.
Frontiers in Immunology
-
Suppression of Tumor Growth in Mice by Rationally Designed Pseudopeptide Inhibitors of Fibroblast Activation Protein and Prolyl Oligopeptidase1
Authors: Kenneth W. Jackson, Victoria J. Christiansen, Vivek R. Yadav, Robert Silasi-Mansat, Florea Lupu, Vibhudutta Awasthi et al.
Neoplasia
-
Area light source‐triggered latent angiogenic molecular mechanisms intensify therapeutic efficacy of adult stem cells
Authors: Yu‐Jin Kim, Sung‐Won Kim, Gwang‐Bum Im, Yeong Hwan Kim, Gun‐Jae Jeong, Hye Ran Jeon et al.
Bioengineering & Translational Medicine
-
Interleukin-1 Induces the Release of Lubricating Phospholipids from Human Osteoarthritic Fibroblast-like Synoviocytes
Authors: Thottakkattumana Parameswaran, V;Hild, C;Eichner, G;Ishaque, B;Rickert, M;Steinmeyer, J;
International journal of molecular sciences
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Goat IgG HRP-conjugated Antibody
Average Rating: 4.9 (Based on 11 Reviews)
Have you used Goat IgG HRP-conjugated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
I had loaded 1μg of recombinant myostatin/GDF8 into a Bio-Rad PROTEAN TGX 4-15% gel, performed electrophoresis with NuPAGE MES buffer and blotted the gel onto 0.2μm nitrocellulose membrane.
For the immuno-detection, I had used the R&D systems goat polyclonal GDF8 antibody (BAF788) at 0.1 or 0.25 µg/mL concentrations, and the R&D system anti-goat secondary antibody (HAF017) at 1:4000 dilution.
Western blot analysis of Human PD-1 in PBMCs using Goat anti-Human PD-1 antibody followed by HRP-conjugated Rabbit anti-Goat secondary antibody (#HAF017) at 1:1,000 dilution in 3% BSA-PBS blocking buffer.
The HRP-conjugated antibody worked as it is supposed to be. No complain. Will buy the product in the future.