HB-2-30

Tocris Bioscience | Catalog # 8831

Fluorescent TG2/LRP1 substrate for imaging endocytosis
Tocris Bioscience

Key Product Details

Description

Fluorescent TG2/LRP1 substrate for imaging endocytosis

Wavelength

Excitation = 649 nm, Emission = 665 nm
View all Fluorescent Lysosome Probes »

Product Description

Key information:

HB-2-30 is a far-red fluorescent transglutaminase-2 (TG2) probe for imaging endocytosis in vitro and in vivo. HB-2-30 undergoes highly efficient endocytosis in complex with TG2 and α2-macroglobulin via the LRP1 pathway.

Used for: Imaging endocytosis in vitro and in vivo, explore targeted lysosomal degradation of secreted and cell surface proteins.

Application: Confocal microscopy.

Properties and Photophysical Data: Suitable for multiplexing and can be combined with DAPI (Cat. No. 5748) counter-staining. Excitation and emission maxima (λ) are 649 and 665 nm, respectively.

Scientific Data Images for HB-2-30

Application of HB-2-30 in vitro

Application of HB-2-30 in vitro.

Confocal microscopy images of bone marrow-derived dendritic cells from WT C57BL/6 mice (left) and TG2KO mice (right) incubated with 3μM HB-2-30. Cells were incubated for 90 minutes with the probe before being fixed, stained for nuclei (blue), and imaged. HB-2-30 signals is in red. Image kindly provided by Fu-Chen Yang and Harrison Besser, University or Stanford.
Application of HB-2-30 in vivo.

Application of HB-2-30 in vivo.

Ileum tissue sections from mice dosed with 10 mg/kg HB-2-30. WT C57BL/6 mice (left) and TG2KO mice (right) were dosed with HB-2-30 and sacrificed after 2 hours. Intestines were Swiss rolled, thin sectioned, stained for nuclei (blue), and imaged via confocal fluorescence microscopy. HB-2-30 signal is in red. Image kindly provided by Fu-Chen Yang and Harrison Besser, University or Stanford.

Licensing Information

Sold under license from Stanford University

Optical Data

HB-2-30 Dye Spectra
λabs 649 nm
λem 665 nm
Application Confocal microscopy

Spectra Viewer

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Product Specifications for HB-2-30

Molecular Weight

1307.59

Formula

C66H86N10O14S2

Storage

Store at -20°C

Purity

≥90% (HPLC)

CAS Number

3031522-14-9

InChI Key

ILCHGSXJNAIGGG-AFAVFPMXSA-N

SMILES

O=C(N1[C@@H](CCC1)C(N[C@H](C(N)=O)CC2=CC=CC=C2)=O)[C@@H](NC([C@H](CC/C=C/C(N(C)C)=O)NC([C@H]3N(CCC3)C(C)=O)=O)=O)CCNC(CCCCC[N+]4=C(/C=C/C=C/C=C5C(C)(C6=CC(S(=O)(O)=O)=CC=C6N\5C)C)C(C)(C)C7=CC(S(=O)([O-])=O)=CC=C47)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solubility Soluble to 13.08mg/ml in DMSO

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Background References

References are publications that support the biological activity of the product.

Product Documents for HB-2-30

Certificate of Analysis

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Product Specific Notices for HB-2-30

For research use only

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