HCoV-HKU1 Human Coronavirus Spike RBD Antibody

Catalog #: MAB10904 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10904-100
MAB10904-SP

Detection of HCoV-HKU1 Spike RBD by Western Blot.

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All Spike RBD Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

HCoV-HKU1 Human Coronavirus Spike RBD Antibody Summary

Species Reactivity

HCoV-HKU1

Specificity

Detects Human coronavirus HCoV-HKU1 Spike RBD in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 1045316

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human embryonic kidney cell HEK293-derived HCoV-HKU1Spike RBD protein
Accession # Q5MQD0.1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

Recombinant HCoV-HKU1 S1 RBD

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of HCoV-HKU1 Spike RBD by Western Blot. Western blot shows recombinant SARS-CoV-2 Spike S1 RBD, recombinant HCoV-HKU1 S1 RBD, recombinant HCoV-NL63 S1 RBD, recombinant HCoV-229E S1 RBD, and recombinant HCoV-OC43 S. PVDF membrane was probed with 2 µg/mL of Mouse Anti-HCoV-HKU1 Spike RBD Monoclonal Antibody (Catalog # MAB10904) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for HCoV-HKU1 Spike RBD at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Spike RBD

HCoV-HKU1 was identified in Hong Kong in 2005 as a new human coronavirus (1). Coronaviruses are a family of viruses that are commonly comprised of a large plus-strand RNA genome and four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein(N). There are two well-known human coronavirus families that infect humans: Alpha coronaviruses which includes HCoV-229E and HCoV-NL63; beta coronaviruses that includes HCov-OC43, Severe Acute Respiratory Syndrome (SARS-CoV), Middle East Respirator Syndrome (MERS-CoV), and global pandemic Covid-19 (SARS-CoV2) (2). The HCoV-HKU1 Spike Protein (S Protein) is a glycoprotein that mediates membrane fusion and viral entry. As with most coronaviruses, proteolytic cleavage of the S protein generates two distinct peptides, S1 and S2 subunits. The S1 subunit is focused on attachment of the protein to the host receptor, while the S2 subunit is involved with cell fusion. The receptor binding domain (RBD) of HCoV-HKU1 is located at C‑terminal region of S1 subunit, similar to SARS‑COV, MERS‑COV and SARS‑COV2, but the RBD regions do not share significant amino acid sequence identity (3). HCoV‑HKU1 has been demonstrated to bind specifically to 9‑O‑acetylated sialic acids (9-O-Ac-Sias) attached as terminal residues to glycan chains on glycoproteins and lipids, but additional receptors remain unknown (4). HCoV‑HKU1, along with HCov-OC43, differ from other cornonaviruses in that their virions possess two types of surface projections, both involved in attachment: large "spikes" that are comprised of homotrimers of the S protein, and unique, smaller protrusions, comprised of the homodimeric hemagglutinin‑esterase (HE) (5).

References

Woo, P. et al. (2005) J. Virol.79:884.
Ogimi, C. et al. (2020) J Pediatric Infect Dis Soc doi: 10.1093/jpids/piaa037.
Qian, Z. et al. (2015) J. Virol. 89:8816.
Huang, X. et al. (2015) J Virol 89:7202.
Hulswit, R.J.G. et al. (2019) PNAS 116:2681.