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HIV-1 Gag p24 Quantikine ELISA Kit

Catalog #: DHP240B
11 Citations 1 Review Datasheet / COA / SDS
Validated for Cell Culture Supernates Only
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DHP240B
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HIV-1 Gag p24 Quantikine ELISA Standard Curve
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Product Details
Citations (11)
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Reviews (1)
Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

HIV-1 Gag p24 Quantikine ELISA Kit Summary

Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL)
Sensitivity
3.35 pg/mL
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates)
Specificity
This assay recognizes HIV-1 Gag p24.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine™ HIV-1 Gag p24 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure HIV-1 Gag p24 in cell culture supernates.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 45.7 135 280 43 132 285
Standard Deviation 1.41 4.86 8.1 1.93 4.61 11.2
CV% 3.1 3.6 2.9 4.5 3.5 3.9

Recovery

The recovery of HIV-1 Gag p24 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 100 93-105

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of HIV-1 Gag p24 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
HIV-1 Gag p24 Quantikine ELISA Linearity Curve

Scientific Data

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HIV-1 Gag p24 Quantikine ELISA Standard Curve

Product Datasheets

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Product Datasheet
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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: HIV-1 Gag p24

The gag gene of human immunodeficiency virus 1 (HIV-1) encodes a precursor protein known as Pr55Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins which are required for virus particle assembly. p24 is a major viral core structural protein. Its measurement is commonly used as an indicator of HIV-1 infection and viral load.

Long Name:
Human Immunodeficiency Virus Type 1 GAG/Group-specific Antigen core protein of 24 kDa
Entrez Gene IDs:
155030 (Viral)
Alternate Names:
CA; Capsid Protein; HIV1 Gag p24; HIV-1 Gag p24; Pr55(Gag)

Citations for HIV-1 Gag p24 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Serological study of CoronaVac vaccine and booster doses in Chile: immunogenicity and persistence of anti-SARS-CoV-2 spike antibodies
    Authors: L Vargas, N Valdivieso, F Tempio, V Simon, D Sauma, L Valenzuela, C Beltrán, L Castillo-D, X Contreras-, ML Acevedo, J Acevedo, RI Gonzalez, F Valiente-E, R Soto-Rifo, M Rosemblatt, M Lopez, F Osorio, MR Bono
    Bmc Medicine, 2022-06-09;20(1):216.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Differential neutralizing antibody responses elicited by CoronaVac and BNT162b2 against SARS-CoV-2 Lambda in Chile
    Authors: ML Acevedo, A Gaete-Arge, L Alonso-Pal, MM de Oca, A Bustamante, A Gaggero, F Paredes, CP Cortes, S Pantano, C Martínez-V, J Angulo, N Le Corre, M Ferrés, MA Navarrete, F Valiente-E, R Soto-Rifo
    Nature Microbiology, 2022-04-01;7(4):524-529.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Epitranscriptomic regulation of HIV-1 full-length RNA packaging
    Authors: C Pereira-Mo, D Toro-Ascuy, C Ananías-Sá, A Gaete-Arge, C Rojas-Fuen, S Riquelme-B, B Rojas-Aray, F García-de-, P Aguilera-C, J Chnaiderma, ML Acevedo, F Valiente-E, R Soto-Rifo
    Nucleic Acids Research, 2022-02-28;0(0):.
    Species: Human
    Sample Types: Whole Cells
  4. Multiple expansions of globally uncommon SARS-CoV-2 lineages in Nigeria
    Authors: EA Ozer, LM Simons, OM Adewumi, AA Fowotade, EC Omoruyi, JA Adeniji, OA Olayinka, TJ Dean, J Zayas, PP Bhimalli, MK Ash, AI Maiga, AM Somboro, M Maiga, A Godzik, JR Schneider, JI Mamede, BO Taiwo, JF Hultquist, R Lorenzo-Re
    Nature Communications, 2022-02-03;13(1):688.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. CCR5 antagonist reduces HIV-induced amyloidogenesis, tau pathology, neurodegeneration, and blood-brain barrier alterations in HIV-infected hu-PBL-NSG mice
    Authors: B Bhargavan, SM Woollard, JE McMillan, GD Kanmogne
    Molecular Neurodegeneration, 2021-11-22;16(1):78.
    Species: Mouse
    Sample Types: Plasma
  6. High prevalence of SARS-CoV-2 B.1.1.7 (UK variant) and the novel B.1.5.2.5 lineage in Oyo State, Nigeria
    Authors: EA Ozer, LM Simons, OM Adewumi, AA Fowotade, EC Omoruyi, JA Adeniji, TJ Dean, J Zayas, PP Bhimalli, MK Ash, A Godzik, JR Schneider, JI Mamede, BO Taiwo, JF Hultquist, R Lorenzo-Re
    medRxiv : the preprint server for health sciences, 2021-07-02;0(0):.
    Species: Human
    Sample Types: Virus
  7. Plasmacytoid dendritic cells have divergent effects on HIV infection of initial target cells and induce a pro-retention phenotype
    Authors: O Tong, G Duette, TR O'Neil, CM Royle, H Rana, B Johnson, N Popovic, S Dervish, MAE Brouwer, H Baharlou, E Patrick, G Ctercteko, S Palmer, E Lee, E Hunter, AN Harman, AL Cunningham, N Nasr
    PloS Pathogens, 2021-04-19;17(4):e1009522.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. A New Generation of Functional Tagged Proteins for HIV Fluorescence Imaging
    Authors: JI Mamede, J Griffin, S Gambut, TJ Hope
    Viruses, 2021-02-28;13(3):.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Insights into neutralizing antibody responses in individuals exposed to SARS-CoV-2 in Chile
    Authors: C Beltrán-Pa, S Riquelme-B, A Oyarzún-Ar, A Gaete-Arge, R González-S, K Cereceda-S, A Aguirre, D Travisany, R Palma-Veja, GP Barriga, A Gaggero, C Martínez-V, NL Corre, M Ferrés, ME Balcells, J Fernandez, E Ramírez, F Villarroel, F Valiente-E, R Soto-Rifo
    Science Advances, 2021-02-12;7(7):.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Epigenetics, N-myrystoyltransferase-1 and casein kinase-2-alpha modulates the increased replication of HIV-1 CRF02_AG, compared to subtype-B viruses
    Authors: B Bhargavan, GD Kanmogne
    Sci Rep, 2019-07-23;9(1):10689.
    Species: Human
    Sample Types: Plasma
  11. Automation of human pluripotent stem cell differentiation toward retinal pigment epithelial cells for large-scale productions
    Authors: F Regent, L Morizur, L Lesueur, W Habeler, A Plancheron, K Ben M'Bare, C Monville
    Sci Rep, 2019-07-23;9(1):10646.
    Species: Human
    Sample Types: Plasma

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HIV-1 Gag p24 Quantikine ELISA Kit
By Anonymous on 12/21/2020
Sample Tested: 293T human embryonic kidney cell line

I used this kit to quantify HIV-1 Gag VLPs in cell culture supernatants. I adsorb the VLPs on polymer membranes and desorb them by vortexing/ centrifugation. I use HIV-1 Gag p24 ELISA kit to determine how polymer surface chemistry can be manipulated to control reversible adsorption and desorption of VLPs.

HIV-1 Gag p24 Quantikine ELISA Kit DHP240B