Hoechst Janelia Fluor® 646
Chemical Name: 2-(3-(Azetidin-1-ium-1-ylidene)-7-(azetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)-4-((2-(2-(2-(4-(4-(5-(4-methylpiperazin-1-yl)-1H,3'H-[2,5'-bibenzo[d]imidazol]-2'-yl)phenoxy)butanamido)ethoxy)ethoxy)ethyl)carbamoyl)benzoate
Purity: ≥90%
Biological Activity
Key information: Hoechst Janelia Fluor® 646 is a fluorogenic red-emitting DNA probe. Suitable for fixed and live-cell imaging.Application:confocal microscopy, super resolution microscopy (SRM) (including PALM and PAINT), lattice light sheet microscopy.
Properties and Photophysical Data: Hoechst Janelia Fluor® 646 preferentially stains and binds minor groove of AT-rich regions. The compound can be combined with fluorogenic green-emitting DNA probe Hoechst Janelia Fluor® 526 (Cat. No. 7313) for multiplexing experiments. Hoechst Janelia Fluor® 646 can also be combined with Hoechst Janelia Fluor® 526 to perform dual-color STED using the same depletion laser (λdep = 775 nm). Sub-diffraction spatial resolution STED imaging can be achieved using Hoechst Janelia Fluor® 646 in E. coli cells (~100 nM - 1 μM). Hoechst Janelia Fluor® 646 is a desirable alternative to large oligonucleotide-conjugated antibodies for PAINT experiments, particularly for bacterial studies. Excitation and emission maxima (λ) are 655 nm and 670 nm, respectively; quantum yield = 0.54; extinction coefficient = 152,000 M-1cm-1.
Hoechst Janelia Fluor® 646 is fluorogenic and fluoresces only once bound to DNA, enabling hassle-free no-wash experiments.
To measure the absorbance spectrum of Hoechst Janelia Fluo® 646 we recommend the following solvent: TFE plus 0.1% TFA.
Scientific Data
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Application of Hoechst Janelia Fluor® 646 in U2OS cells. Fixed U2OS cells were stained with Hoechst Janelia Fluor® 646 (1 µM) for 1 hour and imaged without an intermediate washing step on a LSM980 confocal microscope (Zeiss) using the following configuration: excitation: 633 nm / emission: 638–759 nm. Image kindly provided by Prof. Luke Lavis, Howard Hughes Medical Institute, Janelia Research Campus.
Optical Data for Hoechst Janelia Fluor® 646
| Emission Color | Red |
|---|---|
| Brightness | |
| λabs | 655 nm |
| λem | 670 nm |
| Extinction Coefficient (ε) | 152,000 M-1cm-1 |
| Quantum Yield (φ) | 0.54 |
| Closest Laser line | 640 nm |
| Cell Permeable | Yes |
| Application | Super Resolution Microscopy (PALM, PAINT), Confocal Microscopy, Lattice Light Sheet Microscopy |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Additional Information
Background References
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A general method to fine-tune fluorophores for live-cell and in vivo imaging.
Grimm et al.
Nat.Methods, 2017;14:987
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