Human 17 beta -HSD1/HSD17B1 Antibody

Catalog #: AF7178 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7178
AF7178-SP

Detection of Human 17 beta -HSD1/HSD17B1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human 17 beta -HSD1/HSD17B1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human 17 beta -HSD1/HSD17B1 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human 17 beta -HSD1/HSD17B1
Ala2-Gln328
Accession # P14061

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Simple Western

2.5 µg/mL

Human placenta

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human 17 beta -HSD1/HSD17B1 by Western Blot. Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human 17 beta -HSD1/HSD17B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7178) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for 17 beta -HSD1/HSD17B1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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17 beta ‑HSD1/HSD17B1 in Human Alzheimer's Brain. 17 beta -HSD1/HSD17B1 was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Sheep Anti-Human 17 beta -HSD1/HSD17B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7178) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western

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Detection of Human 17 beta ‑HSD1/HSD17B1 by Simple Western^TM. Simple Western lane view shows lysates of human placenta, loaded at 0.2 mg/mL. A specific band was detected for 17 beta ‑HSD1/HSD17B1 at approximately 40 kDa (as indicated) using 2.5 µg/mL of Sheep Anti-Human 17 beta ‑HSD1/HSD17B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7178) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 17 beta-HSD1/HSD17B1

HSD17B1 (Hydroxysteroid 17-beta dehydrogenase type 1; also 17 beta ‑HSD1 and E2DH) is a 33-34 kDa member of the short chain dehydrogenase/reductase (SDR) superfamily of molecules. It is a cytoplasmic breast, placental and gonadal enzyme that both converts DHEA to estrogen A-diol, and inactivates DHT through oxidation and reduction. Human HSD17B1 is 328 amino acids (aa) in length. It contains an NADP binding site (aa 10-38) and a steroid catalytic site (aa 210-221). HSD17B1 functions as a 68 kDa noncovalently-linked homodimer. Over aa 2-328, human HSD17B1 shares 65% aa identity with mouse HSD17B1.

Long Name

17 beta Hydroxysteroid Dehydrogenase 1

Entrez Gene IDs

3292 (Human); 15485 (Mouse); 25322 (Rat)

Alternate Names

17 betaHSD1; 17 beta-HSD1; 17-beta-HSD 1; 17-beta-hydroxysteroid dehydrogenase type 1; 20 alpha-hydroxysteroid dehydrogenase; 20-alpha-HSD; E17KSR; E2DH; EC 1.1.1.62; EDH17B1; EDH17B2; EDH17B2EDHB17; EDHB17; estradiol 17-beta-dehydrogenase 1; estradiol 17-beta-dehydrogenase-1; HSD17; HSD17B1; hydroxysteroid (17-beta) dehydrogenase 1 isoform; hydroxysteroid (17-beta) dehydrogenase 1; MGC138140; Placental 17-beta-hydroxysteroid dehydrogenase; SDR28C1; short chain dehydrogenase/reductase family 28CE, member 1